CAJ | 학술논문

目的探讨脑胶质瘤组织中生存素和磷酸化信号传导与转录激活因子3(p-STAT3)表达与相互关系及其在脑胶质瘤发生发展过程中的作用.方法应用免疫组织化学即用型二步法检测62例脑胶质瘤和10例正常脑组织中生存素和p-STAT3的表达情况.结果生存素和p-STAT3在正常脑组织中不表达;在脑胶质瘤中,生存素和P-STAT3高表达,阳性率分别为56.45%、61.29%,Kernohan分级Ⅲ+Ⅳ级与Ⅰ+Ⅱ级生存素和p-STAT3阳性表达差异有统计学意义(P<0.05);在35例生存素阳性表达病例中,p-STAT3表达阳性25例(71.43%),生存素蛋白与p-STAT3蛋白的表达呈正相关(r=0.38,P=0.003).结论生存素基因可能是通过p-STAT3蛋白的激活在脑胶质瘤组织中发挥抗凋亡作用,促进脑胶质瘤发生及发展.
목적 탐토뇌효질류조직중생존소화린산화신호전도여전록격활인자3(p-STAT3)표체여상호관계급기재뇌효질류발생발전과정중적작용.방법 응용면역조직화학즉용형이보법검측62례뇌효질류화10례정상뇌조직중생존소화p-STAT3적표체정황.결과 생존소화p-STAT3재정상뇌조직중불표체;재뇌효질류중,생존소화P-STAT3고표체,양성솔분별위56.45%、61.29%,Kernohan분급Ⅲ+Ⅳ급여Ⅰ+Ⅱ급생존소화p-STAT3양성표체차이유통계학의의(P<0.05);재35례생존소양성표체병례중,p-STAT3표체양성25례(71.43%),생존소단백여p-STAT3단백적표체정정상관(r=0.38,P=0.003).결론 생존소기인가능시통과p-STAT3단백적격활재뇌효질류조직중발휘항조망작용,촉진뇌효질류발생급발전.
Objective To investigate the expressions of survivin and p-STAT3 in the tumor tissue of brain glioma, their corrlations and the role in the development and progression of brain glioma. Methods Simple two-step immunohistochemical staining was used for the measurement of the expressions of the survivin and p-STAT3 be-tween 62 cases of brain glioma and 10 normals. Results No expressions of the survivin and p-STAT3 were noted in the normal brain tissue, while high expressions of them were done in brain glioma, with the positive rate of 56.45 and 61.29% respectively. There was a markedly significant difference in the expressions of the survivin and p-STAT3 between Kernohan's Grade 3-4 and 1-2, P<0. 05. Of 35 survivin-positive cases, 25(71.43%) were p-STAT3-positive, and the expression of the survivin protein was directly related to that of p-STAT3 (r =0. 38, P =0.003). Conclusion Survivin gene may play the anti-apoptotic function in the turmor tissue of brain glioma through activating the p-STAT3 protein, faciliating the development and progression of the glioma.