中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
7期
1321-1323
,共3页
吕会增%李玺%陈图峰%叶小勇%周如健
呂會增%李璽%陳圖峰%葉小勇%週如健
려회증%리새%진도봉%협소용%주여건
全反式维甲酸%人结肠癌细胞亚株%细胞周期%凋亡
全反式維甲痠%人結腸癌細胞亞株%細胞週期%凋亡
전반식유갑산%인결장암세포아주%세포주기%조망
All-trans retinoic acid%Human colorectal cancer cell subline%Cell cycle%Apoptosis
目的 观察全反式维甲酸(ATRA)对人结肠癌细胞亚株SW480/M5增殖和凋亡的影响.方法 噻唑蓝(MTT)比色法检测1、2、4、8μmol/L ATRA作用24、48、72 h对SW480/M5细胞的抑制率.流式细胞仪检测8 μmol/L ATRA作用24、48、72 h及2、4μmol/L ATRA作用48 h的肿瘤细胞周期和凋亡率.结果 ATRA抑制SW480/M5细胞增殖作用呈一定时效和量效依赖性;8μmol/LATRA作用72 h明显抑制肿瘤细胞增殖,抑制率接近30%.2、4μmol/L ATRA作用SW480/M5细胞48 h或8μmol/L作用24 h,肿瘤细胞G1期比例开始降低,S期比例增加(P<0.05).8umol/L ATRA作用48 h后,S期比例进一步增高,G2/M期细胞比例不增加;作用72 h后,G1期细胞比例进一步下降,伴G2/M期细胞比例增加(P<0.05).8μmol/L ATRA作用24 h无明显诱导SW480/M5细胞凋亡作用,作用48 h或72 h可诱导肿瘤细胞凋亡,但凋亡率差异无统计学意义(P>0.05).结论 8μmoL/LATRA作用48 h或72 h通过阻滞SW480/M5细胞在S期或(和G2/M)期,并诱导肿瘤细胞凋亡,可明显抑制肿瘤细胞增殖.
目的 觀察全反式維甲痠(ATRA)對人結腸癌細胞亞株SW480/M5增殖和凋亡的影響.方法 噻唑藍(MTT)比色法檢測1、2、4、8μmol/L ATRA作用24、48、72 h對SW480/M5細胞的抑製率.流式細胞儀檢測8 μmol/L ATRA作用24、48、72 h及2、4μmol/L ATRA作用48 h的腫瘤細胞週期和凋亡率.結果 ATRA抑製SW480/M5細胞增殖作用呈一定時效和量效依賴性;8μmol/LATRA作用72 h明顯抑製腫瘤細胞增殖,抑製率接近30%.2、4μmol/L ATRA作用SW480/M5細胞48 h或8μmol/L作用24 h,腫瘤細胞G1期比例開始降低,S期比例增加(P<0.05).8umol/L ATRA作用48 h後,S期比例進一步增高,G2/M期細胞比例不增加;作用72 h後,G1期細胞比例進一步下降,伴G2/M期細胞比例增加(P<0.05).8μmol/L ATRA作用24 h無明顯誘導SW480/M5細胞凋亡作用,作用48 h或72 h可誘導腫瘤細胞凋亡,但凋亡率差異無統計學意義(P>0.05).結論 8μmoL/LATRA作用48 h或72 h通過阻滯SW480/M5細胞在S期或(和G2/M)期,併誘導腫瘤細胞凋亡,可明顯抑製腫瘤細胞增殖.
목적 관찰전반식유갑산(ATRA)대인결장암세포아주SW480/M5증식화조망적영향.방법 새서람(MTT)비색법검측1、2、4、8μmol/L ATRA작용24、48、72 h대SW480/M5세포적억제솔.류식세포의검측8 μmol/L ATRA작용24、48、72 h급2、4μmol/L ATRA작용48 h적종류세포주기화조망솔.결과 ATRA억제SW480/M5세포증식작용정일정시효화량효의뢰성;8μmol/LATRA작용72 h명현억제종류세포증식,억제솔접근30%.2、4μmol/L ATRA작용SW480/M5세포48 h혹8μmol/L작용24 h,종류세포G1기비례개시강저,S기비례증가(P<0.05).8umol/L ATRA작용48 h후,S기비례진일보증고,G2/M기세포비례불증가;작용72 h후,G1기세포비례진일보하강,반G2/M기세포비례증가(P<0.05).8μmol/L ATRA작용24 h무명현유도SW480/M5세포조망작용,작용48 h혹72 h가유도종류세포조망,단조망솔차이무통계학의의(P>0.05).결론 8μmoL/LATRA작용48 h혹72 h통과조체SW480/M5세포재S기혹(화G2/M)기,병유도종류세포조망,가명현억제종류세포증식.
Objective To investigate roles of all-trans retinoic acid (ATRA) in human colorectal cancer cell subline SW480/M5.Methods The effect of ATRA at 1,2,4,8 μmol/L for 24,48,72 h inhibiting cell growth by % in SW480/M5 cells was determined by methyl thiazol tetrazolium (MTT) assay.The cell cycle and apoptosis induced by ATRA at 8 μmol/L for 24,48,72 h respectively or at 2,4μmol/L for 48 h was assessed by flow cytometry.Results In SW480/M5 cells,ATRA was a dose-and time-dependent drug,which had significant growth inhibitory activity nearly at 30% against SW480/M5cells after a 72 h continuous treatmentat 8 μmol/L.With ATRA at 2 or 4 μmol/L for 48 h or 8 μmol/L for 24 h in SW480/M5 cells,the percentage of cells in G1 phase was reduced and increased was that in Sphase ( P <0.05).With ATRA at 8 μmol/L in SW480/M5 cells for 48 h,the percentage of cells in G1 phase was reduced,in addtion that in S-phase was increased significantly and that in G2/M-phase was not increased than the wild cancer cells (P<0.05 ) ; With ATRA at 8 μmol/L in SW480/M5 cells for 72h,the percentages of reduced cells in G1-phase were more than that for 48h and the percentages of increased cells in G2/M-phase were more than that of the wild cancer cells ( P < 0.05 ) ; With ATRA at 8 μmol/L for 24 h in SW480/M5 cells,the cells were not induced to apoptosis obviously.The apoptosis rate of SW480/M5 cells induced significantly by ATRA at 8μmol/L for 48h were just same as that for 72 h (P>0.05 ).Conclusion ATRA at 8μmol/L for 48h or 72h can significantly inhibit SW480/M5 cells growth by blocking the cells into S-phase or (and into G2/M-phase) and inducing the cells apoptosis.