CAJ | 학술논문

目的观察甲基强的松龙与地塞米松对C6胶质瘤大鼠放射性脑损伤后脑水肿程度的影响以及对血液中性粒细胞CDl8 mRNA表达水平的改变.方法雄性Wistar大鼠100只,体质量250～300 g,随机分成5组,每组20只,分别为接受照射同时30 ms/kg体重甲基强的松龙或10 ms/ks体重甲基强的松龙或5 ms/kg体重地塞米松治疗组(A、B、C组),接受照射但未接受激素治疗的实验对照组(D),未接受照射和激素治疗的正常对照组(E).各组均颅内种植肿瘤,ABCD组于种植15 d后予60Co照射,同时自照射前3 d开始静脉注射激素或生理盐水,直至照射后6 d,照射后第7天取大鼠脑组织测含水量,颈总动脉取血,离心取中性粒细胞层,逆转录.聚合酶链反应(RlT-PCR)分析测定血液中性粒细胞CDl8 mRNA表达水平.结果脑组织含水量与血液中性粒细胞CDl8 mRNA表达水平:D组、E组分别与ABC组比较差异均有统计学意义(P<0.05);D E组两组间比较,差异有统计学意义(P<0.05);c组分别与A B组比较,差异均有统计学意义(P<0.05);A、B两组间比较,差异无统计学意义(P>0.05).结论甲基强的松龙与地塞米松均可通过抑制CD18表达的方式减轻放射性脑水肿,但甲基强的松龙效果较好.
목적 관찰갑기강적송룡여지새미송대C6효질류대서방사성뇌손상후뇌수종정도적영향이급대혈액중성립세포CDl8 mRNA표체수평적개변.방법 웅성Wistar대서100지,체질량250～300 g,수궤분성5조,매조20지,분별위접수조사동시30 ms/kg체중갑기강적송룡혹10 ms/ks체중갑기강적송룡혹5 ms/kg체중지새미송치료조(A、B、C조),접수조사단미접수격소치료적실험대조조(D),미접수조사화격소치료적정상대조조(E).각조균로내충식종류,ABCD조우충식15 d후여60Co조사,동시자조사전3 d개시정맥주사격소혹생리염수,직지조사후6 d,조사후제7천취대서뇌조직측함수량,경총동맥취혈,리심취중성립세포층,역전록.취합매련반응(RlT-PCR)분석측정혈액중성립세포CDl8 mRNA표체수평.결과 뇌조직함수량여혈액중성립세포CDl8 mRNA표체수평:D조、E조분별여ABC조비교차이균유통계학의의(P<0.05);D E조량조간비교,차이유통계학의의(P<0.05);c조분별여A B조비교,차이균유통계학의의(P<0.05);A、B량조간비교,차이무통계학의의(P>0.05).결론 갑기강적송룡여지새미송균가통과억제CD18표체적방식감경방사성뇌수종,단갑기강적송룡효과교호.
Objective To study the effect of methylprednisolone or Dexamethasone on radioactive cerebral edema and the expression of CD18 in the rat neutrophilic granulocytes. Methods One hundred male Wistar rats were divided into 5 groups at random ( n =20 in each group). Group A was irradiated and treated with 30 mg/kg methylprednisolone ,group B was irradiated and treated with 10 mg/kg methylprednisolone,group C was irradiated and treated with 5 mg/kg Dexamethasone, group D was irradiated and treated without glueoeortieoid, and group E served as normal controls. Glioma cells were implanted into brains of rats in groups A, B, C, D and E. The rats in groups A, B, C and D were subjected to60 Co radiation 15 days after tumor implantation. All groups were administered with glucocorticoids or normal saline at 3rd day before and at 14th day after radiation. The water content of the brain tissue in rots was measured at 7th day after radiation. The expression of CD18 in the neutrophils of rats was detected. Results There was significant difference in the water content of the brain tissues and the level of CD18 mRNA expression between groups D,E and groups A,B,C (P <0.05) ,group D and group E (P <0.05) ,group C and group A ( P < 0.05 ), but there was no significant difference between groups A and B ( P > 0.05 ). Conclusion Both methylprednisolone and Dexamethasone can protect the radiation-induced injury and brain edema changes by inhibiting the expression of CDI8 in rats, and the methylprednisolone is superior to Dexamethasone.