中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
6期
691-693
,共3页
梅玫%任玉%周旋%祁艳斌%姚智
梅玫%任玉%週鏇%祁豔斌%姚智
매매%임옥%주선%기염빈%요지
聚酰胺%5-氟尿嘧啶%小RNA-21%乳腺癌
聚酰胺%5-氟尿嘧啶%小RNA-21%乳腺癌
취선알%5-불뇨밀정%소RNA-21%유선암
Polyamide%5-Fu%miR-21 inhibitor%Breast carcinoma
目的 观察聚酰胺-氨(PAMAM)纳米微粒介导5-氟尿嘧啶(5-Fu)联合小RNA-21抑制乳腺癌细胞MCF-7生长的效果.方法 透析法制备5-Fu/PAMAM,孵育法同载miR-21抑制剂;透射电镜观察形貌;紫外分光光度计检测载药率和包封率;流式细胞仪检测转染效率及细胞凋亡;噻唑蓝(MTT)比色法检测细胞增殖;Transwell检测细胞侵袭能力变化.结果 5-Fu/PAMAM形貌规整,包封率为(66.21±4.11)%,载药率为(31.77±0.73)%,转染效率为(60.54±6.97)%;联合治疗组肿瘤细胞生长缓慢,在观察截止期时生存率(55.85±3.71)%;联合治疗组细胞凋亡率(18.32±2.42)%,与对照组比较明显增多(F=58.326,P<0.01);联合治疗组视野内细胞数目仅为(18.96±3.14)个,侵袭能力显著降低(F=16.409,P<0.01).结论 PAMAM可以实现5-Fu和miR-21的同载,并有效抑制乳腺癌细胞的体外生长.
目的 觀察聚酰胺-氨(PAMAM)納米微粒介導5-氟尿嘧啶(5-Fu)聯閤小RNA-21抑製乳腺癌細胞MCF-7生長的效果.方法 透析法製備5-Fu/PAMAM,孵育法同載miR-21抑製劑;透射電鏡觀察形貌;紫外分光光度計檢測載藥率和包封率;流式細胞儀檢測轉染效率及細胞凋亡;噻唑藍(MTT)比色法檢測細胞增殖;Transwell檢測細胞侵襲能力變化.結果 5-Fu/PAMAM形貌規整,包封率為(66.21±4.11)%,載藥率為(31.77±0.73)%,轉染效率為(60.54±6.97)%;聯閤治療組腫瘤細胞生長緩慢,在觀察截止期時生存率(55.85±3.71)%;聯閤治療組細胞凋亡率(18.32±2.42)%,與對照組比較明顯增多(F=58.326,P<0.01);聯閤治療組視野內細胞數目僅為(18.96±3.14)箇,侵襲能力顯著降低(F=16.409,P<0.01).結論 PAMAM可以實現5-Fu和miR-21的同載,併有效抑製乳腺癌細胞的體外生長.
목적 관찰취선알-안(PAMAM)납미미립개도5-불뇨밀정(5-Fu)연합소RNA-21억제유선암세포MCF-7생장적효과.방법 투석법제비5-Fu/PAMAM,부육법동재miR-21억제제;투사전경관찰형모;자외분광광도계검측재약솔화포봉솔;류식세포의검측전염효솔급세포조망;새서람(MTT)비색법검측세포증식;Transwell검측세포침습능력변화.결과 5-Fu/PAMAM형모규정,포봉솔위(66.21±4.11)%,재약솔위(31.77±0.73)%,전염효솔위(60.54±6.97)%;연합치료조종류세포생장완만,재관찰절지기시생존솔(55.85±3.71)%;연합치료조세포조망솔(18.32±2.42)%,여대조조비교명현증다(F=58.326,P<0.01);연합치료조시야내세포수목부위(18.96±3.14)개,침습능력현저강저(F=16.409,P<0.01).결론 PAMAM가이실현5-Fu화miR-21적동재,병유효억제유선암세포적체외생장.
Objective To study the effect of PAMAM-mediated 5-fluorouracil combined with miR-21 inhibitor gene therapy to suppress MCF-7 human breast cancer cell growth in vitro. Methods 5-Fu/PAMAM complex was prepared by dialysis method and then incubated with miR-21 inhibitor at room temperature. Transmission electronic microscopy (TEM) was performed to observe the morphology of the nanoparticles. The drug loading efficiency and encapsulation efficiency was determined by ultraviolet spectroscopy (UV). The transfection of PAMAM dendrimer was detected by flow cytometry. MTT assay was carried out to determine MCF-7 cell growth survival rate. Cell apoptosis was analyzed by flow-cytometry. Transwell assay was performed to detect invasion ability after MCF-7 cells treated with 5-Fu chemotherapy combined with miR-21 inhibitor gene therapy. Results The morphology of the complex was sphere observed under TEM. Encapsulation efficiency and loading efficiency of drug were (66. 21±4. 11)% and (31.77±0. 73)% , respectively. Flow cytometry revealed that 5-Fu/PAMAM transfection efficiency was (60.54 ±6. 97)%. 5-Fu combined with miR-21 inhibitor treatment significantly suppressed cell growth, and the survival rate was only (55. 85±3. 71)% on the 6th day of the observation period. The apoptosis rate in combined treatment group was (18. 32±2.42)% , dramatically higher than in control group (F=58. 326,P<0. 01). In combined treatment group, the number of invasion cells was only 18. 96 ±3. 14, suggesting the greatly decreased invasion ability of MCF-7 cells (F=16. 409,P < 0. 01). Conclusion PAMAM could effectively deliver miR-21 inhibitor and 5-Fu simultaneously, and combined therapy can suppress growth of MCF-7 cells effectively in vitro.