CAJ | 학술논문

目的探讨脱细胞基质( DCM)人工血管用于小口径血管移植的可行性.方法 40条雄性杂种犬随机分为DCM、膨体聚四氟乙烯(ePTFE)人工血管及自体颈外静脉3组行右颈总动脉置换术,彩超监测移植物通畅率.术后4、8周活体取材,标本行苏木素-伊红(HE)、免疫组织化学染色及扫描电镜检查.结果 3组移植物1周通畅率(75.0％、64.3％、100.0％)差异无统计学意义(P＞0.05)；自体颈外静脉组4、8周通畅率( 100.0％、88.9％)优于DCM组(56.3％、26.7％)及ePTFE组(57.1％、23.1％,P＜0.05),后两组差异无统计学意义(P＞0.05).DCM人工血管4、8周血栓形成面积小于ePTFE人工血管,吻合口内膜内皮化程度高于后者.结论小口径DCM人工血管在抑制血栓形成及加快内皮化方面优于ePTFE人工血管.
목적 탐토탈세포기질( DCM)인공혈관용우소구경혈관이식적가행성.방법 40조웅성잡충견수궤분위DCM、팽체취사불을희(ePTFE)인공혈관급자체경외정맥3조행우경총동맥치환술,채초감측이식물통창솔.술후4、8주활체취재,표본행소목소-이홍(HE)、면역조직화학염색급소묘전경검사.결과 3조이식물1주통창솔(75.0％、64.3％、100.0％)차이무통계학의의(P＞0.05)；자체경외정맥조4、8주통창솔( 100.0％、88.9％)우우DCM조(56.3％、26.7％)급ePTFE조(57.1％、23.1％,P＜0.05),후량조차이무통계학의의(P＞0.05).DCM인공혈관4、8주혈전형성면적소우ePTFE인공혈관,문합구내막내피화정도고우후자.결론 소구경DCM인공혈관재억제혈전형성급가쾌내피화방면우우ePTFE인공혈관.
Objective To explore the feasibility of smal-caliber decellularized matrix (DCM) vascular grafts in vascular transplantation.Methods 40 male dogs were randomly divided into three groups,and underwent right common carotid artery interposition with DCM (group Ⅰ,n =16),expanded polytetrafluoroethylene (ePTFE) (group Ⅱ,n =14 ) vascular grafts or autologous external jugular vein (group Ⅲ,n =10).Postoperative Doppler Ultrasound were done to detect the patency.Grafts were explanted at 4 and 8 weeks after implantation.The retrieved specimens were evaluated by morphology,hematoxylin and eosin (HE) stain,immunohistochemistry stain,and scanning electron microscopy.Results The patency rates at 1 week among the three groups (75.0％,64.3％,100.0％ respectively) were of no difference of significance (P ＞0.05).4 and 8 week patency rates of group Ⅲ ( 100.0％,88.9％ )are both higher than group Ⅰ (56.3％,26.7％ ) and group Ⅱ (57.1％,23.1％,P ＜0.05 ),however,no differences of significance were gained between the latter two (P ＞ 0.05).The thrombus formation area of DCM grafts was smaller than that of ePTFE grafts.DCM grafts achieved better endothelialization in the stomas neointima than ePTFE grafts within 8 weeks.Conclusion Compared with ePTFE grafts,DCM grafts show superiority in reducing thrombogeneity,and improving endothelialization.