CAJ | 학술논문

目的观察甲氨蝶呤(MTx)对人骨肉瘤MG-63细胞凋亡及Livin和Caspase-3表达的影响.方法体外培养骨肉瘤MG-63细胞株,用0、50、100、200和400μmol/L的MTX作用于骨肉瘤MG-63细胞24、48、72 h后,用噻唑蓝(MTT)比色法检测骨肉瘤MG-63细胞的增殖活性,用流式细胞仪测细胞的凋亡率,用Western blot检测不同浓度MTX作用于骨肉瘤MG-63细胞24 h后各组细胞的Livin和Caspase-3蛋白表达水平.结果随MTX浓度增加骨肉瘤MG-63细胞的增殖活性降低,同时细胞的凋亡率增加(P＜0.05),随MTX浓度增加各组细胞中Livin蛋白的表达降低,Caspase-3蛋白表达增加(P＜0.05).结论 MTX诱导人骨肉瘤MG-63细胞凋亡,其机制可能与下调Livin表达继而上调Caspase-3表达有关.
목적 관찰갑안접령(MTx)대인골육류MG-63세포조망급Livin화Caspase-3표체적영향.방법 체외배양골육류MG-63세포주,용0、50、100、200화400μmol/L적MTX작용우골육류MG-63세포24、48、72 h후,용새서람(MTT)비색법검측골육류MG-63세포적증식활성,용류식세포의측세포적조망솔,용Western blot검측불동농도MTX작용우골육류MG-63세포24 h후각조세포적Livin화Caspase-3단백표체수평.결과 수MTX농도증가골육류MG-63세포적증식활성강저,동시세포적조망솔증가(P＜0.05),수MTX농도증가각조세포중Livin단백적표체강저,Caspase-3단백표체증가(P＜0.05).결론 MTX유도인골육류MG-63세포조망,기궤제가능여하조Livin표체계이상조Caspase-3표체유관.
Objective To observe the effect of Methotrexate (MTX) on apoptosis and expression of Livin and Caspase-3 in human osteosarcoma cell line MG-63. Methods After treatment of MG-63 cells with MTX at different concentrations (0, 50, 100,200,400 μmol/L) for 24, 48 and 72 h, methyl thiazol tetrazolium(MTT) assay was used to observe the growth inhibition of MG-63. The apoptosis was assessed by flow cytometry. The protein expression of Livin and Caspase-3 was detected by Western blotting. Results When MTX was added, growth inhibition and increased apoptosis of MG-63 cells were detected,which was showed in a dose- and time-dependent manner. MTX also down-regulated the level of the protein expression of Livin (P＜0.05), and elevated the protein expression of Caspase-3 (P＜0.05). Conclusion MTX can induce apoptosis of MG-63 cells, by down-regulating Livin expression and subsequently up-regulating Caspase-3 expression.