CAJ | 학술논문

目的探讨SLP-2蛋白在食管鳞状细胞癌组织中的表达及其与食管鳞状细胞癌临床病理特征的关系.方法收集18例新鲜配对的食管鳞状细胞癌及正常食管上皮组织,应用Western blot法检测SLP-2蛋白的表达水平.将220例配对的食管鳞状细胞癌组织和正常食管上皮组织构建组织芯片,采用免疫组化SP法检测SLP-2蛋白的表达情况,并分析SLP-2蛋白的表达与食管鳞状细胞癌临床病理特征之间的关系.结果 Western blot法检测结果显示,SLP-2蛋白在18例新鲜食管鳞状细胞癌组织中的高表达率为72.2%.免疫组化染色结果显示,SLP-2蛋白在220例食管鳞状细胞癌组织中的高表达率为54.1%,明显高于其在正常食管上皮组织中的高表达率(3.6%,P＜0.001).SLP-2蛋白的高表达与食管鳞状细胞癌的浸润深度相关(P=0.033),而与其他临床病理特征均无关(均P＞0.05).结论 SLP-2可能作为一个新的肿瘤相关基因在食管鳞状细胞癌的发生发展过程中发挥着重要作用;SLP-2蛋白的高表达与食管鳞状细胞癌的侵袭转移密切相关.
목적 탐토SLP-2단백재식관린상세포암조직중적표체급기여식관린상세포암림상병리특정적관계.방법 수집18례신선배대적식관린상세포암급정상식관상피조직,응용Western blot법검측SLP-2단백적표체수평.장220례배대적식관린상세포암조직화정상식관상피조직구건조직심편,채용면역조화SP법검측SLP-2단백적표체정황,병분석SLP-2단백적표체여식관린상세포암림상병리특정지간적관계.결과 Western blot법검측결과현시,SLP-2단백재18례신선식관린상세포암조직중적고표체솔위72.2%.면역조화염색결과현시,SLP-2단백재220례식관린상세포암조직중적고표체솔위54.1%,명현고우기재정상식관상피조직중적고표체솔(3.6%,P＜0.001).SLP-2단백적고표체여식관린상세포암적침윤심도상관(P=0.033),이여기타림상병리특정균무관(균P＞0.05).결론 SLP-2가능작위일개신적종류상관기인재식관린상세포암적발생발전과정중발휘착중요작용;SLP-2단백적고표체여식관린상세포암적침습전이밀절상관.
Objective To study the expression of stomatin-like protein-2 (SLP-2) in esophageal squamous cell carcinoma (ESCC), and analyze the correlation between SLP-2 expression and clinicopathological features. Methods The expression of SLP-2 protein in ESCC tissues ( 18 and 220 cases respectively) was detected by Western blot and IHC. The association between SLP-2 expression and clinicopathological features was analyzed. Results Compared with normal epithelium, 13 cases of ESCC tissues showed a higher expression of SLP-2 on the protein level (72.2%, 13/18). IHC analysis on tissue microarray revealed that the expression rate of SLP-2 protein in ESCC was 54. 1% and in normal esophageal mucosa was 3.6%, showing a significant difference ( P ＜0.001 ). SLP-2 high-level expression correlates with the extent of ESCC invasion ( P = 0. 033 ), but not with other clinicopathologic characteristics ( P ＞0.05). Conclusion SLP-2 as a novel cancer-related gene may play an important role in tumorigenesis of ESCC. The overexpression of SLP-2 may be closely associated with the invasion of esophageal cancer.