CAJ | 학술논문

为了解pgx4基因在尖孢镰刀菌古巴专化型侵染香蕉过程中的作用,及其与尖孢镰刀菌古巴专化型生理小种1号和生理小种4号之间的致病力差异的关系,以尖孢镰刀菌古巴专化型1号生理小种和4号生理小种的基因组DNA和cDNA为材料,采用PCR和RT-PCR方法扩增了2个生理小种的pgx4基因,并对扩增产物进行克隆测序后再用生物信息学软件对序列进行比对分析.结果表明:2个生理小种pgx4基因的开放阅读框均为1 395 bp,编码465个氨基酸,且前17个氨基酸均构成该基因的信号肽.通过DNAStar 7.1比对发现,尖孢镰刀菌古巴专化型2个生理小种pgx4基因的核苷酸序列存在25个碱基的差异,而其编码的465个氨基酸中,也有3个氨基酸不同,分析这些核苷酸序列和氨基酸序列的差异可能与尖孢镰刀菌古巴专化型1号生理小种和4号生理小种在侵染蕉类不同栽培种时的识别专性寄主机制有一定关系.
위료해pgx4기인재첨포렴도균고파전화형침염향초과정중적작용,급기여첨포렴도균고파전화형생리소충1호화생리소충4호지간적치병력차이적관계,이첨포렴도균고파전화형1호생리소충화4호생리소충적기인조DNA화cDNA위재료,채용PCR화RT-PCR방법확증료2개생리소충적pgx4기인,병대확증산물진행극륭측서후재용생물신식학연건대서렬진행비대분석.결과표명:2개생리소충pgx4기인적개방열독광균위1 395 bp,편마465개안기산,차전17개안기산균구성해기인적신호태.통과DNAStar 7.1비대발현,첨포렴도균고파전화형2개생리소충pgx4기인적핵감산서렬존재25개감기적차이,이기편마적465개안기산중,야유3개안기산불동,분석저사핵감산서렬화안기산서렬적차이가능여첨포렴도균고파전화형1호생리소충화4호생리소충재침염초류불동재배충시적식별전성기주궤제유일정관계.
In order to understand the role of pgx4 gene in the invasion process of Fusarium oxysporum f. sp. cubense (Foc) which causes Panama disease in banana, and the relation of pgxA with the pathogenic difference between race one and race four of F. oxysporum f. sp. cubense infecting the same banana variety. Races one and four of F. oxysporum f. sp. cubense were used as materials to extract genomic DNA and cDNA from which pathogenicity -related genes pgx4 of F. oxysporum f. sp. cubense were amplified by using Polymerase Chain Reaction and Reverse Transcription Polymerase Chain Reaction. The amplified products were cloned and sequenced, and sequence alignments were done by using the biological softwares. The results revealed that the open reading frames of pgxA genes from both races one and four were 1 395 bp and encoded 465 amino acids and that the first 17th amino acids consisted of signal peptides of these genes. Alignment with DNAStar7.1 found that These 2 races had 25 different bases in nucleotides of the gene pgx4 between each other, and also contained 3 different amino acids among the amino acids encoded in these genes. The sequences alignment result and the amino acid sequence showed that the difference in nucleotide and amino acid sequences between races one and four might had some relationship with host specific recognition of races one and four in attacking different banana varieties.