中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2011年
7期
712-716
,共5页
史晶%胡春林%高渝峰%廖晓星
史晶%鬍春林%高渝峰%廖曉星
사정%호춘림%고투봉%료효성
百草枯%急性中毒%血小板内皮细胞黏附分子-1%急性肺损伤
百草枯%急性中毒%血小闆內皮細胞黏附分子-1%急性肺損傷
백초고%급성중독%혈소판내피세포점부분자-1%급성폐손상
Paraquat%Acute poisoning%PECAM-1%Acute lung injury
目的 探讨百草枯中毒后肺组织内血小板内皮细胞黏附分子-1(PECAM-1)表达的变化与肺损伤和肺纤维化程度的关系.方法 36只成年新西兰大白兔,依据胃管内灌入百草枯剂量的不同,随机分为8 mg/kg(A组)、16 mg/kg(B组)和32 mg/kg(C组).中毒后观察动物生存状况;7 d后处死动物,取肺组织进行组织学损伤评分、Masson染色鉴定肺纤维化(LF)程度、免疫组化半定量分析PECAM-1的表达,Pearson相关分析法确定PECAM-1表达和ALI、LF程度的关系.结果 每组12只动物,均出现明显的中毒症状,C组存活时间为(6.47±0.99)d,短于B组的(6.09±1.04)d(P=0.031)和A组的(4.77±2.04)d(P=0.007).ALI评分A组为(8.33±1.03),低于B组的(9.83±1.17)(P=0.047)和C组的(11.50±1.38)(P＜0.01),B组与C组相比较,P=0.03.肺纤维化程度A组为(31.09±2.05)%,低于B组的(34.37±1.62)%(P=0.002)和C组的(36.54±0.44)%(P＜0.01),B组和C组之间差异具有统计学意义(P=0.026).A组肺PECAM-1的表达为(20.31±0.70)%,高于B组的(19.34±0.68)%(P=0.16)和C组的(18.37±0.46)%(P＜0.01),B组与C组相比,差异具有统计学意义(P=0.017).Pearson相关分析显示,PECAM-1的表达和ALI评分(Coe=-0.732,P=0.001)、肺纤维化程度(Coe=-0.779,P＜0.001)明显相关.结论 新西兰兔PQ中毒后,肺组织内PECAM-1表达明显下降,成剂量依赖性,与肺损伤程度、肺纤维化程度密切相关;PECAM-1表达的下降在PQ致肺损伤的发生、发展中起重要作用.
目的 探討百草枯中毒後肺組織內血小闆內皮細胞黏附分子-1(PECAM-1)錶達的變化與肺損傷和肺纖維化程度的關繫.方法 36隻成年新西蘭大白兔,依據胃管內灌入百草枯劑量的不同,隨機分為8 mg/kg(A組)、16 mg/kg(B組)和32 mg/kg(C組).中毒後觀察動物生存狀況;7 d後處死動物,取肺組織進行組織學損傷評分、Masson染色鑒定肺纖維化(LF)程度、免疫組化半定量分析PECAM-1的錶達,Pearson相關分析法確定PECAM-1錶達和ALI、LF程度的關繫.結果 每組12隻動物,均齣現明顯的中毒癥狀,C組存活時間為(6.47±0.99)d,短于B組的(6.09±1.04)d(P=0.031)和A組的(4.77±2.04)d(P=0.007).ALI評分A組為(8.33±1.03),低于B組的(9.83±1.17)(P=0.047)和C組的(11.50±1.38)(P＜0.01),B組與C組相比較,P=0.03.肺纖維化程度A組為(31.09±2.05)%,低于B組的(34.37±1.62)%(P=0.002)和C組的(36.54±0.44)%(P＜0.01),B組和C組之間差異具有統計學意義(P=0.026).A組肺PECAM-1的錶達為(20.31±0.70)%,高于B組的(19.34±0.68)%(P=0.16)和C組的(18.37±0.46)%(P＜0.01),B組與C組相比,差異具有統計學意義(P=0.017).Pearson相關分析顯示,PECAM-1的錶達和ALI評分(Coe=-0.732,P=0.001)、肺纖維化程度(Coe=-0.779,P＜0.001)明顯相關.結論 新西蘭兔PQ中毒後,肺組織內PECAM-1錶達明顯下降,成劑量依賴性,與肺損傷程度、肺纖維化程度密切相關;PECAM-1錶達的下降在PQ緻肺損傷的髮生、髮展中起重要作用.
목적 탐토백초고중독후폐조직내혈소판내피세포점부분자-1(PECAM-1)표체적변화여폐손상화폐섬유화정도적관계.방법 36지성년신서란대백토,의거위관내관입백초고제량적불동,수궤분위8 mg/kg(A조)、16 mg/kg(B조)화32 mg/kg(C조).중독후관찰동물생존상황;7 d후처사동물,취폐조직진행조직학손상평분、Masson염색감정폐섬유화(LF)정도、면역조화반정량분석PECAM-1적표체,Pearson상관분석법학정PECAM-1표체화ALI、LF정도적관계.결과 매조12지동물,균출현명현적중독증상,C조존활시간위(6.47±0.99)d,단우B조적(6.09±1.04)d(P=0.031)화A조적(4.77±2.04)d(P=0.007).ALI평분A조위(8.33±1.03),저우B조적(9.83±1.17)(P=0.047)화C조적(11.50±1.38)(P＜0.01),B조여C조상비교,P=0.03.폐섬유화정도A조위(31.09±2.05)%,저우B조적(34.37±1.62)%(P=0.002)화C조적(36.54±0.44)%(P＜0.01),B조화C조지간차이구유통계학의의(P=0.026).A조폐PECAM-1적표체위(20.31±0.70)%,고우B조적(19.34±0.68)%(P=0.16)화C조적(18.37±0.46)%(P＜0.01),B조여C조상비,차이구유통계학의의(P=0.017).Pearson상관분석현시,PECAM-1적표체화ALI평분(Coe=-0.732,P=0.001)、폐섬유화정도(Coe=-0.779,P＜0.001)명현상관.결론 신서란토PQ중독후,폐조직내PECAM-1표체명현하강,성제량의뢰성,여폐손상정도、폐섬유화정도밀절상관;PECAM-1표체적하강재PQ치폐손상적발생、발전중기중요작용.
Objective To explore the relationships between the expression of PECAM-1 and the degree of ALI in Paraquat induced lung injury model of rabbits. Method Thirty six adult New Zealand rabbits were randomly divided into three groups: 8 mg/kg (Group A), 16 mg/kg (Group B) and 32 mg/kg ( Group C) according to the dose of Paraquat which were infusion into stomach. After poisoned, the animals were monitored for seven days, and then sacrificed. The upper lobe of lung were removed for HE,Masson staining and immunohistochemisty. The ALl score, fibrosis of lung and expression of PECAM-1 were semiquantitative analyzed. Results Each group has 12 animals suffered from poisoning. The survival time of animals in Group C was (6. 47 ± 0. 99 ) days, shorter than (6. 09 + 1.04) days ( P = 0. 031 ) in Group B and (4. 77 + 2. 04) days ( P = 0. 0 07) in Group A. The ALI score were ( 8. 33 ± 1.03) points in Group A, superior to (9. 83 ± 1.17) points ( P = 0. 047 ) in Group B and ( 11.50 + 1.38) points ( P ＜ 0. 01 ) in group C, Group B vs Group C, P=-0.03o The fibrosis degree of lung was (31.09 +2.05)% in Group A,not severe as (34. 37 ±1.62)% (P=0. 002) in Group B and (36. 54 ±0. 44)% (P ＜0. 01 ) in Group C, Group B vs Group C, P = 0. 026. The Pearson correlation analysis showed the expression of PECAM-1 was negative correlated to ALI score (Coe = -0. 732, P =0. 001 ) and fibrosis degree of lung (Coe = -0. 779, P ＜ 0. 001 ) . Conclusions The expressions of PECAM-1 were significantly decreased in New Zealand after Paraquat poisoned, which were dose dependent, correlated to ALI scored and fibrosis degree of lung, so it may play an important role in the development of lung injured induced by Paraquat.
