CAJ | 학술논문

目的建立声诱发短潜伏期负电位(acoustically evoked short latency negative response,ASNR)豚鼠模型,即重度感音性聋但球囊功能正常的豚鼠,用短声刺激诱发ASNR,并通过冷热试验及前庭诱发肌源性电位(vestibular evoked myogenic potential,VEMP)来验证豚鼠的前庭功能.方法将32只健康豚鼠按随机数字表法分为两组,对照组16只(32耳)、药物致聋组16只(32耳).致聋组经给药(硫酸卡那霉素+利尿酸)致聋,7～10 d后对所有动物进行听觉前庭功能检查,包括听性脑干反应(ABR)、VEMP及冷热试验.致聋组豚鼠根据ASNR的引出情况分为ASNR引出组及ASNR未引出组.结果致聋组有11只动物(22耳)完成测试,其中8耳引出ASNR(36.4%),阈值为120～130 dB SPL,平均(124.4±5.0)dB SPL,潜伏期l.75～2.60 ms,平均(2.15±0.27)ms,阈值平均潜伏期(2.34±0.18)ms.ASNR引出组8耳皆引出VEMP,其阈值及正负峰潜伏期与对照组比较,差异均无统计学意义(P值均＞0.05);ASNR未引出组中有4耳引出VEMP,其阈值及正负峰潜伏期与对照组比较差异亦无统计学意义(P值均＞0.05).ASNR引出组和未引出组VEMP引出率比较,差异具有统计学意义(P=0.002).致聋组VEMP、ASNR的引出情况与冷热试验的眼震结果之间均无相关关系(P值均＞0.05).结论 ASNR与反映球囊功能的VEMP具有一致性,而与代表半规管功能的冷热试验眼震结果无关,ASNR与VESP可能同起源于球囊.
목적 건립성유발단잠복기부전위(acoustically evoked short latency negative response,ASNR)돈서모형,즉중도감음성롱단구낭공능정상적돈서,용단성자격유발ASNR,병통과랭열시험급전정유발기원성전위(vestibular evoked myogenic potential,VEMP)래험증돈서적전정공능.방법 장32지건강돈서안수궤수자표법분위량조,대조조16지(32이)、약물치롱조16지(32이).치롱조경급약(류산잡나매소+이뇨산)치롱,7～10 d후대소유동물진행은각전정공능검사,포괄은성뇌간반응(ABR)、VEMP급랭열시험.치롱조돈서근거ASNR적인출정황분위ASNR인출조급ASNR미인출조.결과 치롱조유11지동물(22이)완성측시,기중8이인출ASNR(36.4%),역치위120～130 dB SPL,평균(124.4±5.0)dB SPL,잠복기l.75～2.60 ms,평균(2.15±0.27)ms,역치평균잠복기(2.34±0.18)ms.ASNR인출조8이개인출VEMP,기역치급정부봉잠복기여대조조비교,차이균무통계학의의(P치균＞0.05);ASNR미인출조중유4이인출VEMP,기역치급정부봉잠복기여대조조비교차이역무통계학의의(P치균＞0.05).ASNR인출조화미인출조VEMP인출솔비교,차이구유통계학의의(P=0.002).치롱조VEMP、ASNR적인출정황여랭열시험적안진결과지간균무상관관계(P치균＞0.05).결론 ASNR여반영구낭공능적VEMP구유일치성,이여대표반규관공능적랭열시험안진결과무관,ASNR여VESP가능동기원우구낭.
Objective To establish a model of acoustically evoked short latency negative response (ASNR) in guinea pigs, a model of profound hearing loss with normal saccular functions, and verify the correlation between ASNR and vestibular evoked myogenic potential (VEMP). Methods Thirty-two healthy guinea pigs were employed in the experiment, which were randomly divided into control group ( 16subjects) and deafened group (16 subjects). Each animal experienced auditory and vestibular tests including auditory brainstem response ( ABR), VEMP and caloric test. A quick treatment was employed for deafened group consisting of a subcutaneous injection of kanamycin at a dose of 400 mg/kg followed by a jugular vein injection of ethacrynic acid at a dose of 40 mg/kg one hour later. The animals were received ABR, VEMP and caloric test 7 - 10 days following the drug administration. The deafened group was further divided into ASNR group and non-ASNR group, based on the presence of ASNR. Results In deafened group, five subjects died postoperatively, 11 subjects (22 ears) provided full data, ASNR was elicited in eight ears (36.4%), the threshold was 120- 130 dB SPL with mean of (124.4 ±4.96) dB SPL. Its latency range was 1.75 - 2. 60 ms with mean of ( 2. 15 ± 0. 27 ) ms. The mean latency of threshold was (2. 34 ±0. 18) ms. All eight ASNR ears presented with VEMP. The VEMP threshold, positive and negative potential latencies proved no statistical difference (P ＞ 0. 05 ) between ASNR group and control group.Significant difference was detected between the VEMP presence of ASNR group and non-ASNR group ( P =0. 002). There was no statistically significant correlation between VEMP and caloric test neither between ASNR and caloric test in deafened group. Conclusions This study evoked ASNR in an ototoxicity guinea pig model which has profound hearing loss with normal saccular functions. The presence of ASNR correlated with VEMP, however, not correlated with caloric test, suggesting that ASNR and VEMP are both originated from the saccule.