CAJ | 학술논문

目的应用简便快速的核酸检测新方法--逆转录环介导等温扩增方法(RT-LAMP)检测麻疹病毒核酸,并与巢式逆转录多聚酶链式反应(nest-RT-PCR)方法进行比较.方法比较RT-LAMP方法与nest-RT-PCR方法对吉林省历年麻疹病毒分离株以及临床疑似麻疹咽试子中麻疹病毒核酸检测的检出率.结果两种方法对23株麻疹分离株麻疹病毒核酸阳性检出率均达到100%.针对18份麻疹病毒分离阴性的临床疑似麻疹咽试子分别利用RT-LAMP和巢式RT-PCR两种方法进行麻疹核酸检测,RT-LAMP方法阳性率为56.52%,巢式RT-PCR方法阳性率为47.83%.结论 RT-LAMP方法比巢式nest-RT-PCR方法更敏感.
목적 응용간편쾌속적핵산검측신방법--역전록배개도등온확증방법(RT-LAMP)검측마진병독핵산,병여소식역전록다취매련식반응(nest-RT-PCR)방법진행비교.방법 비교RT-LAMP방법여nest-RT-PCR방법대길림성력년마진병독분리주이급림상의사마진인시자중마진병독핵산검측적검출솔.결과 량충방법대23주마진분리주마진병독핵산양성검출솔균체도100%.침대18빈마진병독분리음성적림상의사마진인시자분별이용RT-LAMP화소식RT-PCR량충방법진행마진핵산검측,RT-LAMP방법양성솔위56.52%,소식RT-PCR방법양성솔위47.83%.결론 RT-LAMP방법비소식nest-RT-PCR방법경민감.
Objective A new simple RT-LAMP method was applied to detect measles vires nucleic acid and compared with nest-RT-PCR. Methods Compare the detection rate of the RT-LAMP method with that of nest-RT-PCR by detecting measles virus nucleic acid from measles virus and clinical samples. Results The nucleic acid positive rotes of all 23 strains of measles virus are all 100% by the two methods. But to the detection of 18 clinical samples which are negative in measles isolation,the nest-RT-LAMP showed 56.52 % positive rate of nucleic acid of measles virus and nest-RT-PCR showed 47.83 %. Conclusion RT-LAMP is more sensitive than nest-RT-PCR.