中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2011年
2期
125-128
,共4页
基因%血管紧张素Ⅱ%肌,平滑,血管
基因%血管緊張素Ⅱ%肌,平滑,血管
기인%혈관긴장소Ⅱ%기,평활,혈관
Gene%AngiotensinⅡ%Muscle,smooth,vascular
目的 通过血管紧张素转换酶2(ACE2)基因转染大鼠血管平滑肌细胞(VSMC),观察其对血管紧张素Ⅱ(AngⅡ)促VSMC增殖的影响.方法 将大鼠VSMC分为正常细胞组、AngⅡ组、空载体+AngⅡ组及pm-ACE2+AngⅡ组,通过CCK8细胞计数试剂盒和流式细胞仪检测各组细胞周期,观察ACE2基因转染对AngⅡ促VSMC增殖效应的影响.结果 与正常细胞组相比,AngⅡ组细胞计数明显增高,pm-ACE2+AngⅡ组与AngⅡ组相比显著降低(0.535±0.004比0.866±0.026,P＜0.05);同时,AngⅡ组在G0/G1期细胞的百分率明显降低(58.80%±2.00%,P＜0.05),S期的则显著增高(35.90%±1.00%,P＜0.05),与AngⅡ组相比,pmACE2+AngⅡ组在G0/G1期的百分率明显升高(63.90%±1.40%,P＜0.05),S期则显著降低(27.80%±0.46%,P＜0.05).结论 ACE2基因转染能抑制AngⅡ的促VSMC异常增殖效应.
目的 通過血管緊張素轉換酶2(ACE2)基因轉染大鼠血管平滑肌細胞(VSMC),觀察其對血管緊張素Ⅱ(AngⅡ)促VSMC增殖的影響.方法 將大鼠VSMC分為正常細胞組、AngⅡ組、空載體+AngⅡ組及pm-ACE2+AngⅡ組,通過CCK8細胞計數試劑盒和流式細胞儀檢測各組細胞週期,觀察ACE2基因轉染對AngⅡ促VSMC增殖效應的影響.結果 與正常細胞組相比,AngⅡ組細胞計數明顯增高,pm-ACE2+AngⅡ組與AngⅡ組相比顯著降低(0.535±0.004比0.866±0.026,P＜0.05);同時,AngⅡ組在G0/G1期細胞的百分率明顯降低(58.80%±2.00%,P＜0.05),S期的則顯著增高(35.90%±1.00%,P＜0.05),與AngⅡ組相比,pmACE2+AngⅡ組在G0/G1期的百分率明顯升高(63.90%±1.40%,P＜0.05),S期則顯著降低(27.80%±0.46%,P＜0.05).結論 ACE2基因轉染能抑製AngⅡ的促VSMC異常增殖效應.
목적 통과혈관긴장소전환매2(ACE2)기인전염대서혈관평활기세포(VSMC),관찰기대혈관긴장소Ⅱ(AngⅡ)촉VSMC증식적영향.방법 장대서VSMC분위정상세포조、AngⅡ조、공재체+AngⅡ조급pm-ACE2+AngⅡ조,통과CCK8세포계수시제합화류식세포의검측각조세포주기,관찰ACE2기인전염대AngⅡ촉VSMC증식효응적영향.결과 여정상세포조상비,AngⅡ조세포계수명현증고,pm-ACE2+AngⅡ조여AngⅡ조상비현저강저(0.535±0.004비0.866±0.026,P＜0.05);동시,AngⅡ조재G0/G1기세포적백분솔명현강저(58.80%±2.00%,P＜0.05),S기적칙현저증고(35.90%±1.00%,P＜0.05),여AngⅡ조상비,pmACE2+AngⅡ조재G0/G1기적백분솔명현승고(63.90%±1.40%,P＜0.05),S기칙현저강저(27.80%±0.46%,P＜0.05).결론 ACE2기인전염능억제AngⅡ적촉VSMC이상증식효응.
Objective To investigate the effect of Ang Ⅱ on the proliferation of vascular smooth muscle cell (VSMC) in rats after the transfection of ACE2 gene. Methods pm-ACE2 was transfected into the cultured VSMC by Lipofectamine 2000. The normal cell group, Ang Ⅱ group and pcDNA3. 1/Hygro( + )transfected + Ang Ⅱ group were taken as controls respectively. After the transfection of ACE2 gene, the cell proliferative effect of Ang Ⅱ on VSMC was investigated by cell counting kit-8 (CCK8) and cell cycle detection by fluorescence activated cell sorter (FACS). Results The (optical density) OD value of Ang Ⅱgroup was obviously higher than that of other groups. And it was obviously lower in the pm-ACE2 + Ang Ⅱgroup than the Ang Ⅱ group (0. 535 ± 0. 004 vs 0.866 ± 0.026, P ＜ 0. 05 ). Compared with other groups, the G0/G1 stage percentage of VSMC was obviously lower in the Ang Ⅱ group(58. 80% ±2. 00%, P ＜0. 05)while the percentage of S stage was obviously higher ( 35.90% ± 1.00%, P ＜ 0.05 ). Compared with the Ang Ⅱ group , the G0/G1 stage percentage of VSMC was obviously higher( 63. 90% ± 1.40%, P ＜ 0. 05 ) in the pm-ACE2 + Ang Ⅱ group while the percentage of S stage was obviously lower( 27.80% ± 0. 46%, P ＜0. 05 ). Conclusion The over-expression of ACE2 gene can inhibit the proliferation of Ang Ⅱ-induced VSMC.
