CAJ | 학술논문

为探讨氧化型低密度脂蛋白致动脉粥样硬化的作用是否与基质金属蛋白酶表达活性改变有关，应用Northernblot、Dotblot、Westernblot和明胶酶图分析方法观察氧化型低密度脂蛋白对体外培养的大鼠血管平滑肌细胞表达基质金属蛋白酶-2和9的影响。结果显示，10mg/L氧化型低密度脂蛋白作用于血管平滑肌细胞24h，可明显增强基质金属蛋白酶-2和9mRNA表达、蛋白分泌和酶的活性，高浓度的氧化型低密度脂蛋白对基质金属蛋白酶-2和9表达的影响不同，氧化型低密度脂蛋白浓度为50mg/L时，基质金属蛋白酶-9表达活性仍然高于对照细胞，但与10mg/L氧化型低密度脂蛋白的作用强度无显著差别；相同条件下，基质金属蛋白酶-2的基因表达和蛋白分泌明显降低。以上结果提示，氧化型低密度脂蛋白可诱导血管平滑肌细胞基质金属蛋白酶-2和9的表达，并可促进细胞外基质降解。
위탐토양화형저밀도지단백치동맥죽양경화적작용시부여기질금속단백매표체활성개변유관，응용Northernblot、Dotblot、Westernblot화명효매도분석방법관찰양화형저밀도지단백대체외배양적대서혈관평활기세포표체기질금속단백매-2화9적영향。결과현시，10mg/L양화형저밀도지단백작용우혈관평활기세포24h，가명현증강기질금속단백매-2화9mRNA표체、단백분비화매적활성，고농도적양화형저밀도지단백대기질금속단백매-2화9표체적영향불동，양화형저밀도지단백농도위50mg/L시，기질금속단백매-9표체활성잉연고우대조세포，단여10mg/L양화형저밀도지단백적작용강도무현저차별；상동조건하，기질금속단백매-2적기인표체화단백분비명현강저。이상결과제시，양화형저밀도지단백가유도혈관평활기세포기질금속단백매-2화9적표체，병가촉진세포외기질강해。
Aim To examine the effects of oxidized low density lipoprotein(ox-LDL) on expressions of matrix metalloproteinase-2 (MMP-2) and MMP-9 in cultured rat vascular smooth muscle cells (VSMCs), and explore the relation between the migration and proliferation of VSMCs and the expression activity of these two genes. Methods Northern blot, Dot blot, Western blot and SDS-PAGE containing gelatin were used to detect MMP-2 and MMP-9 mRNA expression, protein synthesis and enzyme activities. Results The mRNA expressions, protein levels and enzyme activities of MMP-2 and MMP-9 significantly increased after VSMCs were stimulated by 10 mg/L ox-LDL for 24 h. When the concentration of ox-LDL were enhanced to 50 mg/L, different effects were exerted on MMP-2 and MMP-9. The levels of MMP-9 were still higher than control group and as the same as the group of 10 mg/L ox-LDL. However, the expression of MMP-2 decreased markedly at the same condition. Conclusion Ox-LDL could increase the expression activities of MMP-2 and MMP-9 and the degradation of ECM.