CAJ | 학술논문

目的建立分析中药三七的HPLC-UV-MS方法, 鉴定三七的化学成分.方法色谱柱: Phenomenex Luna C18 柱 (250 mm×4.6 mm ID, 5 μm); 流动相:0.005%甲酸水溶液(A) 和0.005%甲酸乙腈溶液(B), 梯度洗脱;紫外检测波长范围:195-400 nm; 采用正负离子检测模式.结果三七成分获得了良好的分离与检测.通过与对照品的保留时间、正负离子质谱比较, 鉴定了14个成分, 根据正负离子质谱数据和文献, 分析推断了41个成分, 其中, 野三七皂苷-H、野三七皂苷-E、竹节参皂苷-L5、丙二酰基人参皂苷-Rg1、以及三七皂苷-J、-A、-R1、-G、-R2和人参皂苷-Rh3的异构体为首次在三七中发现.结论本方法灵敏度高、分离度好, 适合三七成分的鉴定.本结果有助于进一步对三七进行活性成分研究及质量控制.
목적건립분석중약삼칠적HPLC-UV-MS방법, 감정삼칠적화학성분.방법색보주: Phenomenex Luna C18 주 (250 mm×4.6 mm ID, 5 μm); 류동상:0.005%갑산수용액(A) 화0.005%갑산을정용액(B), 제도세탈;자외검측파장범위:195-400 nm; 채용정부리자검측모식.결과삼칠성분획득료량호적분리여검측.통과여대조품적보류시간、정부리자질보비교, 감정료14개성분, 근거정부리자질보수거화문헌, 분석추단료41개성분, 기중, 야삼칠조감-H、야삼칠조감-E、죽절삼조감-L5、병이선기인삼조감-Rg1、이급삼칠조감-J、-A、-R1、-G、-R2화인삼조감-Rh3적이구체위수차재삼칠중발현.결론본방법령민도고、분리도호, 괄합삼칠성분적감정.본결과유조우진일보대삼칠진행활성성분연구급질량공제.
Aim To develop a HPLC-UV-MS method for identifying the constituents in the Chinese drug Notoginseng (the root of Panax .6 mm ID, 5 18 column (250 mm×4notoginseng). Methods A Phenomenex Luna Cμm) was utilized. Water containing 0.005% formic acid (A) and acetonitrile containing 0.005% formic acid (B) were used as gradient eluents. UV spectra were recorded in range 195-400 nm. Both positive and negative ion ESI modes were used. Results The constituents in Notoginseng were well separated and detected. Fourteen compounds were identified by comparing their retention time and ESI-MS data with those obtained from the reference compounds. Forty-one compounds were deduced by data analysis of MS and literature; among them, yesanchinosides-H and -E, chikusetsusaponin-L5, malonyl-ginsenoside-Rg1, the isomers of notoginsenosides-J, -A, -R1, -G, -R2, and ginsenoside-Rh3 were discovered in Notoginseng for the first time. Conclusion This method gives high sensitivity and good separation, and is suitable for identifying the constituents in Notoginseng. This result is helpful for further phytochemical research on Notoginseng. Based on this result, further quality control can be studied.