CAJ | 학술논문

选用硝酸和过氧化氧作为消解剂,采用微波技术消解蛹虫草样品,用电感耦合等离子体发射光谱(ICP-AES)法同时测定其中K、P、Ca、Mg、Cu、Fe、Zn、Mn共8种微量元素的含量.进行微波消解条件的优化选择及分析线性、精密度和回收率等实验,建立同时测定蛹虫草中多种微量元素的微波消解-ICP-AES方法.本方法所测元素的标准曲线均有良好的线性关系,以猪肉粉国家标准物质GBW08522为对照进行验证,其回收率为91%～106%,相对标准偏差(RSD)<3.0%;该法加标回收率为90%～108%,RSD<3.0%,具有较好的准确度和精密度,是一种快速、准确而高效的分析方法.测定结果表明,蛹虫草富含有K、P、Mg、Ca、Zn元素.
선용초산화과양화양작위소해제,채용미파기술소해용충초양품,용전감우합등리자체발사광보(ICP-AES)법동시측정기중K、P、Ca、Mg、Cu、Fe、Zn、Mn공8충미량원소적함량.진행미파소해조건적우화선택급분석선성、정밀도화회수솔등실험,건립동시측정용충초중다충미량원소적미파소해-ICP-AES방법.본방법소측원소적표준곡선균유량호적선성관계,이저육분국가표준물질GBW08522위대조진행험증,기회수솔위91%～106%,상대표준편차(RSD)<3.0%;해법가표회수솔위90%～108%,RSD<3.0%,구유교호적준학도화정밀도,시일충쾌속、준학이고효적분석방법.측정결과표명,용충초부함유K、P、Mg、Ca、Zn원소.
A method based on microwave digestion and inductively coupled plasma-atomic emission spectrometry (ICP-AES) was established for simultaneous determination of eight trace elements (K, P, Ca, Mg, Cu, Fe, Zn, Mn) in Cordyceps militaris. Samples were digested with HNO_3 and H_2O_2 as digestion agent under the optimized condition. The working curves were established based on the principle of low detection limits, high sensitivity and less substrate element disturbance. Finally, the contents of above eight inorganic elements in Cordyceps militaris were simultaneously determined under the proposed condition. Good linearity of the working curves for the eight elements was obtained in this study. The recovery rates obtained by determining reference matter (pork powder) method were between 91%-106%, and the relative standard deviations (RSD) were lower than 3.0%. The recovery rates obtained by standard addition method were between 90% - 108%, and RSD were lower than 3.0% likewise. It suggests that this method was accurate and reliable for simultaneous determining the eight trace elements existed in Cordyceps militaris. In addition, the analysis results showed that Cordyceps militaris was rich in inorganic elements such as K, P, Mg, Ca, Zn, especially K and P. Furthermore, the relationship between the essential inorganic elements and its particularly medical effects were discussed in this paper.