CAJ | 학술논문

以30份2007年辽宁省的野生大豆种质资源为材料,利用40对SSR引物进行遗传多样性分析.结果表明:18对SSR引物扩增出129个等位变异,平均每个位点等位变异7.22个,Shannon-Weaver指数变化范围为1.1753～2.1234,平均为1.7285.中部平原半湿润区内的种质数、平均等位变异数和遗传多样性指数最高,其次为东部山地湿润区,西部丘陵半干旱区内分布种质数最少,其平均等位变异数和遗传多样性指数均最低.中部平原半湿润区和东部山地湿润区之间的遗传相似性最高(0.6496),遗传距离最近(0.4314),而西北部平原低丘半湿润区和西部丘陵半干旱区之间的遗传相似性最低(0.4326),遗传距离最远(0.8379).聚类结果看到SSR分子标记的结果与品种的地理来源没有明显的相关性.
이30빈2007년요녕성적야생대두충질자원위재료,이용40대SSR인물진행유전다양성분석.결과표명:18대SSR인물확증출129개등위변이,평균매개위점등위변이7.22개,Shannon-Weaver지수변화범위위1.1753～2.1234,평균위1.7285.중부평원반습윤구내적충질수、평균등위변이수화유전다양성지수최고,기차위동부산지습윤구,서부구릉반간한구내분포충질수최소,기평균등위변이수화유전다양성지수균최저.중부평원반습윤구화동부산지습윤구지간적유전상사성최고(0.6496),유전거리최근(0.4314),이서북부평원저구반습윤구화서부구릉반간한구지간적유전상사성최저(0.4326),유전거리최원(0.8379).취류결과간도SSR분자표기적결과여품충적지리래원몰유명현적상관성.
In order to evaluate the genetic diversity of the wild soybean(Glycine soja) in the different region of Liaoning Province,China,a total of 30 wild soybean accessions from 5 ecological regions(semi-humid zone of plain in the centre,semi-humid zone of low hill in the south,semi-humid zone of plain and low hills in the northwest,semi-arid zone of hill in the west ,and humid zone of mountainous lands in the east,abbreviated as regionⅠ,Ⅱ, Ⅲ, Ⅳ andⅤ,respectively )of Liaoning province in 2007 were genotyped using 40 pair of SSR primers.129 alleles were detected by 18 pair of SSR markers,with an average of 7 2 alleles for each locus.Shannon-Weaver diversity indexes varied from 1.1753 to 2.1234 with averaged 1.7285.The region Ⅰ had the most germplasmas,the alleles and the highest genetic diversity,followed by the region Ⅴ and the region Ⅳ had the least and the lowest.The value of genetic similarity was highest(0.6496)while the value of genetic distance was the lowest(0 4314)between the region Ⅰ and Ⅴ.The value of genetic similarity was the lowest(0.4326)while the value of genetic distance was highest(0.8379)between the region Ⅲ and Ⅳ.There was no relativity between the results by the SSR markers and geographic origin by cluster analysis.