CAJ | 학술논문

应用线性扫描伏安法讨论了牛血清白蛋白(BSA)等电点pI 4.8两侧,即:pH 6.2KH_2PO_4-NaOH缓冲液及pH 4.1 HAc-NaAc缓冲液中,槲皮素(QUE)、QUE-BSA体系的电化学行为,研究了不同pH条件下QUE与BSA的相互作用.在NaOH活化玻碳电极上,QUE均产生一灵敏氧化峰,峰电流较裸玻碳电极增加显著,加入BSA后峰电流降低,峰电位移动不明显,且 QUE、QUE-BSA体系的电极反应均为扩散控制的不可逆过程.计算了两种介质条件下,QUE与BSA的结合数均为2,结合常数分别为2.82×10~10L/mol、7.94X10~7L/mol,结果表明介质条件对相互作用有很大影响.
응용선성소묘복안법토론료우혈청백단백(BSA)등전점pI 4.8량측,즉:pH 6.2KH_2PO_4-NaOH완충액급pH 4.1 HAc-NaAc완충액중,곡피소(QUE)、QUE-BSA체계적전화학행위,연구료불동pH조건하QUE여BSA적상호작용.재NaOH활화파탄전겁상,QUE균산생일령민양화봉,봉전류교라파탄전겁증가현저,가입BSA후봉전류강저,봉전위이동불명현,차 QUE、QUE-BSA체계적전겁반응균위확산공제적불가역과정.계산료량충개질조건하,QUE여BSA적결합수균위2,결합상수분별위2.82×10~10L/mol、7.94X10~7L/mol,결과표명개질조건대상호작용유흔대영향.
The interaction between quercetin (QUE) and bovine serum albumin (BSA) was investigated by linear sweep voltammery in different buffer solutions of different pH. On the glass carbon electrode activated by NaOH solution, QUE yielded an irreversible oxidation peak both in pH 6.2 KH_2PO_4-NaOH and in pH 4.1 HAc-NaAc buffer solutions whose pH values were on both sides of BSA's isoelectrie point 4.8. The peak current increased markedly comparing with bare electrode. With the addition of BSA into the QUE solution, the peak current of QUE reduced without any significant shift of the peak potential.The electrode processes of QUE and QUE-BSA system were both controlled by diffusion. The binding ratio was calculated as 2, and the binding constant were 2. 82 X 10~10 L/mol and 7. 94 X 10~7 L/tool,respectively. The result showed that the interaction was influenced by the condition of the medium.