中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2010年
27期
5119-5122
,共4页
郑遵成%魏开斌%刘峰%刘超%魏树刚%程宗%高瑞%张磊%张昆%匡乃峰%张立清
鄭遵成%魏開斌%劉峰%劉超%魏樹剛%程宗%高瑞%張磊%張昆%劻迺峰%張立清
정준성%위개빈%류봉%류초%위수강%정종%고서%장뢰%장곤%광내봉%장립청
脊髓损伤%功能恢复%神经功能%移植%嗅鞘细胞
脊髓損傷%功能恢複%神經功能%移植%嗅鞘細胞
척수손상%공능회복%신경공능%이식%후초세포
背景:一系列基础研究证实在动物脊髓损伤的模型中,嗅鞘细胞移植能够促进脊髓损伤的再生和恢复脊髓的部分神经功能.部分临床实验证明嗅鞘细胞的移植的确能改善脊髓损伤患者的神经功能,从而改善其生活质量.目的:验证嗅鞘细胞移植对脊髓损伤患者神经功能修复的作用及安全性.方法:取流产胚胎嗅球并消化成为单个嗅鞘细胞,培养纯化2周左右,制成嗅鞘细胞悬液.选择脊髓损伤患者213例,全麻下将制备好的嗅鞘细胞悬液采用区域性多靶点注射方法移植于损伤脊髓的周围.采用ASIA量表对患者移植前1d及移植后3周～2个月进行评分,评价患者脊髓恢复状况.结果与结论:所有患者的脊髓神经功能于术后3周均有不同程度的改变,脊髓功能评分及感觉与运动功能均较移植前明显提高(P<0.001),且随时间延长呈不断改善趋势;最长患者随访5年,未见已恢复的神经功能减退及移植不良反应.证实嗅鞘细胞移植对脊髓损伤患者的神经功能恢复有促进作用,可以部分恢复及改善其部分脊髓神经功能,且治疗方法安全.
揹景:一繫列基礎研究證實在動物脊髓損傷的模型中,嗅鞘細胞移植能夠促進脊髓損傷的再生和恢複脊髓的部分神經功能.部分臨床實驗證明嗅鞘細胞的移植的確能改善脊髓損傷患者的神經功能,從而改善其生活質量.目的:驗證嗅鞘細胞移植對脊髓損傷患者神經功能脩複的作用及安全性.方法:取流產胚胎嗅毬併消化成為單箇嗅鞘細胞,培養純化2週左右,製成嗅鞘細胞懸液.選擇脊髓損傷患者213例,全痳下將製備好的嗅鞘細胞懸液採用區域性多靶點註射方法移植于損傷脊髓的週圍.採用ASIA量錶對患者移植前1d及移植後3週～2箇月進行評分,評價患者脊髓恢複狀況.結果與結論:所有患者的脊髓神經功能于術後3週均有不同程度的改變,脊髓功能評分及感覺與運動功能均較移植前明顯提高(P<0.001),且隨時間延長呈不斷改善趨勢;最長患者隨訪5年,未見已恢複的神經功能減退及移植不良反應.證實嗅鞘細胞移植對脊髓損傷患者的神經功能恢複有促進作用,可以部分恢複及改善其部分脊髓神經功能,且治療方法安全.
배경:일계렬기출연구증실재동물척수손상적모형중,후초세포이식능구촉진척수손상적재생화회복척수적부분신경공능.부분림상실험증명후초세포적이식적학능개선척수손상환자적신경공능,종이개선기생활질량.목적:험증후초세포이식대척수손상환자신경공능수복적작용급안전성.방법:취유산배태후구병소화성위단개후초세포,배양순화2주좌우,제성후초세포현액.선택척수손상환자213례,전마하장제비호적후초세포현액채용구역성다파점주사방법이식우손상척수적주위.채용ASIA량표대환자이식전1d급이식후3주～2개월진행평분,평개환자척수회복상황.결과여결론:소유환자적척수신경공능우술후3주균유불동정도적개변,척수공능평분급감각여운동공능균교이식전명현제고(P<0.001),차수시간연장정불단개선추세;최장환자수방5년,미견이회복적신경공능감퇴급이식불량반응.증실후초세포이식대척수손상환자적신경공능회복유촉진작용,가이부분회복급개선기부분척수신경공능,차치료방법안전.
BACKGROUND: A series of basic researches have confirmed that,the olfactory ensheathing cell transplantation can promote spinal cord regeneration and recover some neurological functions of spinal cord in animal models of spinal cord injury.Some clinical trials also prove that transplantation of olfactory ensheathing cells can indeed improve neurological function in patients with spinal cord injury,and then improve their quality of life.OBJECTIVE: To verify the effectiveness and safety of olfactory ensheathing cell transplantation in repair of neurological function of spinal cord injury patients.METHODS: The aborted embryonic olfactory bulb was collected and digested into single olfactory ensheathing cells.After they were cultured and purified 2 weeks,olfactory ensheathing cell suspension was prepared.A total of 213 cases of spinal cord injury were selected.Under general anesthesia,the prepared olfactory ensheathing cell suspension was injected through several target sites surrounding the injured spinal cord.ASIA scale was used to assay the patients before transplantation,3 weeks to 2 months after transplantation,so as to evaluate spinal cord recovery.RESULTS AND CONCLUSION: The spinal cord nerve function in all patients altered to different degrees at 3 weeks postoperation.Spinal cord function score,the sensory and motor functions were significantly increased compared with preoperation(P < 0.001),and showed a trend of continuous improvement with time; the patients were visited as follow-up for no more than 5 years,and no impairment of the restored nervous function or transplant adverse reactions were observed.It is confirmed that olfactory ensheathing cell transplantation can promote the recovery of nerve function in patients with spinal cord injury,it can restore and improve some spinal cord functions,and the treatment is safe.
