CAJ | 학술논문

目的探讨无创伤双后肢缺血后处理对胰腺移植大鼠血清中TNF-α和NO含量的影响.方法 6只SD大鼠为假手术组(Sham组,n=6);12只糖尿病SD大鼠随机分为缺血再灌注组(I/R组,n=6);无创伤双后肢缺血后处理组(NWLIPO组,n=6).检测各组再灌注前、后血糖;再灌注后2 h血清中TNF-α和NO的含量.结果再灌注后NWLIPO组相对于L/R组血糖低(P＜0.01);再灌注后NWLIPO组较I/R组血清中TNF-α含量低(P＜0.01)、NO含量高(P＜0.01).结论无创伤双后肢缺血后处理可以增加胰腺移植大鼠血清中NO合成,下调TNF-α水平.
목적 탐토무창상쌍후지결혈후처리대이선이식대서혈청중TNF-α화NO함량적영향.방법 6지SD대서위가수술조(Sham조,n=6);12지당뇨병SD대서수궤분위결혈재관주조(I/R조,n=6);무창상쌍후지결혈후처리조(NWLIPO조,n=6).검측각조재관주전、후혈당;재관주후2 h혈청중TNF-α화NO적함량.결과 재관주후NWLIPO조상대우L/R조혈당저(P＜0.01);재관주후NWLIPO조교I/R조혈청중TNF-α함량저(P＜0.01)、NO함량고(P＜0.01).결론 무창상쌍후지결혈후처리가이증가이선이식대서혈청중NO합성,하조TNF-α수평.
Objective To evaluate the effects of non-wounded leg ischemic post-conditioning on the serum TNF-α and NO level of rats undergoing pancreas transplantation. Methods Group Sham consisted of 6 normal SD rats. 12 diabetic SD rats were randomly assigned to 2 groups: I/R group consisted of 6 diabetic rats which received ischemia reperfusion and NWLIPO group consisted of 6 diabetic rats which received ischemic post-conditioning. Blood glucose was measured before and after reperfusion. The level of serum NO and TNF-α was monitored 2 hours after long-time reperfusion. Results The level of blood glucose and TNF-α in NWLIPO group was lower than that in I/R group (P＜0. 01) while the level of NO was higher in NWLIPO group than in I/R group (P＜0.01). Conclusion Non-wounded leg ischemic post-conditioning can increase serum NO synthesis and down-regulate TNF-α.