CAJ | 학술논문

目的建立大鼠高选择性神经损伤联合皮肤缺损模型,研究单纯运动神经或感觉神经损伤对大鼠创面愈合的影响. 方法雄性SD大鼠90只按随机数字表法分为三组,每组30只.包括A组:后根感觉神经切断+后肢皮肤缺损组；B组:前根运动神经切断+后肢皮肤缺损组；C组:假神经切断+后肢皮肤缺损组.伤后2,7,14,21 d测定各组大鼠创面愈合率；伤后1,3,7,14 d取创面皮肤组织采用RT -PCR检测降钙素基因相关肽(CGRP) mRNA的表达情况；3,7,14,21 d取创面皮肤组织免疫组织化学染色检测Bcl -2蛋白的表达水平. 结果伤后2dA、C组创面愈合率高于B组(P＜0.05),A、C组比较差异无统计学意义(P＞0.05)；7,14dC组创面愈合率高于A、B组(P＜0.05),A、B组比较差异无统计学意义(P＞0.05); 21 dB、C组创面愈合率高于A组(P＜0.05),B、C组比较差异无统计学意义(P＞0.05).伤后各组均可见CGRP mRNA的表达,并逐渐增强,伤后1,3dB、C组表达均较A组高(P＜0.05),B、C组比较差异无统计学意义(P＞0.05)；伤后7dC组＞B组＞A组(P＜0.05)；14 d B组＞A组＞C组(P＜0.05).免疫组化染色显示,伤后3d各组Bcl -2蛋白表达差异无统计学意义,伤后7dC组表达较A、B组强(P＜0.05),A、B组比较差异无统计学意义(P＞0.05)；伤后14dC组＞B组＞A组(P＜0.05);21 dB组表达高于A、C组(P＜0.05),A、C组比较差异无统计学意义(P＞0.05). 结论在创面愈合过程中,感觉神经纤维对创面愈合的影响较运动神经纤维显著,神经损伤(尤其是感觉神经)不利于创面愈合,完整的神经支配是创面正常愈合的必需条件.
목적 건립대서고선택성신경손상연합피부결손모형,연구단순운동신경혹감각신경손상대대서창면유합적영향. 방법 웅성SD대서90지안수궤수자표법분위삼조,매조30지.포괄A조:후근감각신경절단+후지피부결손조；B조:전근운동신경절단+후지피부결손조；C조:가신경절단+후지피부결손조.상후2,7,14,21 d측정각조대서창면유합솔；상후1,3,7,14 d취창면피부조직채용RT -PCR검측강개소기인상관태(CGRP) mRNA적표체정황；3,7,14,21 d취창면피부조직면역조직화학염색검측Bcl -2단백적표체수평. 결과 상후2dA、C조창면유합솔고우B조(P＜0.05),A、C조비교차이무통계학의의(P＞0.05)；7,14dC조창면유합솔고우A、B조(P＜0.05),A、B조비교차이무통계학의의(P＞0.05); 21 dB、C조창면유합솔고우A조(P＜0.05),B、C조비교차이무통계학의의(P＞0.05).상후각조균가견CGRP mRNA적표체,병축점증강,상후1,3dB、C조표체균교A조고(P＜0.05),B、C조비교차이무통계학의의(P＞0.05)；상후7dC조＞B조＞A조(P＜0.05)；14 d B조＞A조＞C조(P＜0.05).면역조화염색현시,상후3d각조Bcl -2단백표체차이무통계학의의,상후7dC조표체교A、B조강(P＜0.05),A、B조비교차이무통계학의의(P＞0.05)；상후14dC조＞B조＞A조(P＜0.05);21 dB조표체고우A、C조(P＜0.05),A、C조비교차이무통계학의의(P＞0.05). 결론 재창면유합과정중,감각신경섬유대창면유합적영향교운동신경섬유현저,신경손상(우기시감각신경)불리우창면유합,완정적신경지배시창면정상유합적필수조건.
Objective To establish a rat model of pure sensory nerve injury or pure motor nerve injury combined with skin defect to examine the effect of highly selective nerve injury on skin wound healing. Methods A total of 90 male Sprague-Dawley rats were randomly and equally divided into three different groups including Group A (posterior rhizotomy + cutaneous excision wounding group),Group B (anterior rhizotomy + cutaneous excision wounding group) and Group C ( sham operation + cutaneous excision wounding group).The wound healing rate was detected at days 2,7,14 and 21 after injury.The CGRP mRNA expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) at days 1,3,7 and 14 after injury.The Bcl-2 protein expression was determined by immunohistochemistry at days 3,7,14 and 21 after injury. Results The wound healing rate in the Groups A and C was significantly higher than that in the Group B at day 2 ( P ＜ 0.05 ),with no statistical difference between Group A and Group C (P ＞ 0.05 ).The wound healing rate in the Group C was higher than that in the Groups A and B at days 7 and 14 (P ＜0.05),with no statistical difference between Group A and Group B (P ＞0.05).The wound healing rate in the Groups B and C was higher than that in the Group A at day 21 ( P ＜ O.05 ),with no statistical difference between Group B and Group C ( P ＞ 0.05 ).The CGRP mRNA was expressed in three groups and the expression was up-regulated after wound.The CGRP mRNA expression in the Groups B and C was significantly higher than that in the Group A at days 1,3 and 7,with no statistical difference between Group B and Group C ( P ＞ 0.05 ).The CGRP mRNA expression was distributed as Group C ＞ Group B ＞ Group A at day 7 ( P ＜ 0.05 ) and Group B ＞ Group A ＞ Group C at day 14 (P ＜ 0.05 ).Immunohistochemistry indicated that there was no statistical difference in Bcl-2 protein expression at day 3 after injury in all groups; that the Bcl-2 protien expression in the Group C was significantly higher than that in the Groups A and B at day 7 ( P ＜ 0.05 ),with no statistical difference between Group A and Group B ( P ＞ 0.05 ) ; that the Bcl-2 protien expression was distributed as Group C ＞ Group B ＞ Group A at day 14 ; that the Bclo2 protien expression in the Group B was higher than that in the Groups A and C at day 21 (P ＜0.05),with no statistical difference between Group A and Group C (P ＞ 0.05). Conclusions In wound healing process,the sensory nerve plays a more important role than the motor nerve.Denervation of the nerves ( especially the sensory nerves) is not conducive to wound healing,and complete innervation is necessary for normal wound healing.