CAJ | 학술논문

目的将Arg-Gly-Asp(RGD)肽偶联到壳聚糖(cH)材料表面,并制备成包载质粒DNA的纳米粒子,以未偶连RGD的壳聚糖载质粒DNA作为对照,进行体外内皮细胞转染,观察其是否能提高对内皮细胞的转染效率.方法以1-乙基-3-(3-二甲基氨基丙基)碳化二亚胺盐酸盐(EDC)和N-羟基丁二酰亚胺(NHS)为偶联剂,通过酰胺键将RGD肽偶联到壳聚糖表面,对其进行表征,并以未偶连RGD的壳聚糖作为对照,制备载pEGFP-C1质粒DNA纳米粒子,比较2者对Hy926细胞的转染效率.结果壳聚糖-RGD(CH-RGD)载基因纳米粒子转染Hy926细胞的效率明显高于未偶连RGD的壳聚糖载基因纳米粒子(35.7%vs 14.3%,P<0.001).结论 RGD肽表面修饰壳聚糖载基因纳米粒子可用于体外细胞转染,其对细胞的转染效率明显优于未偶连RGD的壳聚糖.
목적 장Arg-Gly-Asp(RGD)태우련도각취당(cH)재료표면,병제비성포재질립DNA적납미입자,이미우련RGD적각취당재질립DNA작위대조,진행체외내피세포전염,관찰기시부능제고대내피세포적전염효솔.방법 이1-을기-3-(3-이갑기안기병기)탄화이아알염산염(EDC)화N-간기정이선아알(NHS)위우련제,통과선알건장RGD태우련도각취당표면,대기진행표정,병이미우련RGD적각취당작위대조,제비재pEGFP-C1질립DNA납미입자,비교2자대Hy926세포적전염효솔.결과 각취당-RGD(CH-RGD)재기인납미입자전염Hy926세포적효솔명현고우미우련RGD적각취당재기인납미입자(35.7%vs 14.3%,P<0.001).결론 RGD태표면수식각취당재기인납미입자가용우체외세포전염,기대세포적전염효솔명현우우미우련RGD적각취당.
Objective To investigate gene transfection efficiency in cell culture mediated by chitosan Nanoparticles immobilized with Arg-Gly-Asp(arginine-glycine-aspartic acid,RGD)-peptides.Mothods RGD-peptides was immobilized on chitosan by chemical crosslinking. Plasmid pEGFP-Cl was encapsulated into Chitosan-RGD-nanoparticles (Chitosan-RGD-pEGFP-NPs) by complex coacervation method. Chitusan-pEGFP-nanoparticles without RGD were used as the control to evaluate gene tranfection efficiency into Hy926 cells in vitro.Results The transfection efficiency of Chitosan-RGD-pEGFP-NPs is statistically higher than Chitosan-pEGFP-NPs.(35.7% vs 14.3%,P<0.001) Conclusion Chitosan-RGD NPs could act as gene vector to transfect specific gene in vitro and the transfection efficiency is higher than that of non-RGD chitosan nanoparticles.