中国医药
中國醫藥
중국의약
CHINA MEDICINE
2012年
6期
754-756
,共3页
子宫颈鳞癌%抑癌基因%人乳头瘤病毒
子宮頸鱗癌%抑癌基因%人乳頭瘤病毒
자궁경린암%억암기인%인유두류병독
Cervical squamous cell carcinoma%Phosphataseand tensin homology deletedon chromosome ten%Human papilloma virus
目的 探讨抑癌基因人第10号染色体缺失的磷酸酶及张力蛋白同源的基因(PTEN)、人乳头瘤病毒(HPV)与新疆维吾尔族妇女子宫颈病变的相关关系.方法 选取维吾尔族妇女正常或炎症的子宫颈组织标本30例为对照组,宫颈病理标本按病变程度及病理学诊断分为宫颈上皮内瘤样病变(CIN)Ⅰ组、CINⅡ～Ⅲ组各30例、宫颈鳞癌组织30例子宫颈鳞癌组采用免疫组化SP法检测PTEN蛋白表达,采用聚合酶链反应(PCR)检测HPV阳性率.结果 PTEN的蛋白表达率在正常或炎症的子宫颈组织、CIN Ⅰ、CINⅡ～Ⅲ、宫颈鳞癌组织中分别为83.3％ 、73.3％、56.7％、23.3％,子宫颈鳞癌组阳性表达率明显减少,与前3组比较差异有统计学意义(P＜0.05).4组HPV阳性率分别为43.3％、56.7％、76.7％、93.3％,HPV阳性率和子宫颈病变程度呈正相关(P＜0.05).结论 新疆维吾尔族妇女子宫颈病变组织中PTEN蛋白水平表达减少,与其宫颈病变呈负相关关系；是子宫颈组织恶变的信号.HPV感染与子宫颈病变的严重程度呈正相关,HPV感染在子宫颈病变的发展过程中起到了极大的促进作用,是子宫颈癌致病因素之一.
目的 探討抑癌基因人第10號染色體缺失的燐痠酶及張力蛋白同源的基因(PTEN)、人乳頭瘤病毒(HPV)與新疆維吾爾族婦女子宮頸病變的相關關繫.方法 選取維吾爾族婦女正常或炎癥的子宮頸組織標本30例為對照組,宮頸病理標本按病變程度及病理學診斷分為宮頸上皮內瘤樣病變(CIN)Ⅰ組、CINⅡ～Ⅲ組各30例、宮頸鱗癌組織30例子宮頸鱗癌組採用免疫組化SP法檢測PTEN蛋白錶達,採用聚閤酶鏈反應(PCR)檢測HPV暘性率.結果 PTEN的蛋白錶達率在正常或炎癥的子宮頸組織、CIN Ⅰ、CINⅡ～Ⅲ、宮頸鱗癌組織中分彆為83.3％ 、73.3％、56.7％、23.3％,子宮頸鱗癌組暘性錶達率明顯減少,與前3組比較差異有統計學意義(P＜0.05).4組HPV暘性率分彆為43.3％、56.7％、76.7％、93.3％,HPV暘性率和子宮頸病變程度呈正相關(P＜0.05).結論 新疆維吾爾族婦女子宮頸病變組織中PTEN蛋白水平錶達減少,與其宮頸病變呈負相關關繫；是子宮頸組織噁變的信號.HPV感染與子宮頸病變的嚴重程度呈正相關,HPV感染在子宮頸病變的髮展過程中起到瞭極大的促進作用,是子宮頸癌緻病因素之一.
목적 탐토억암기인인제10호염색체결실적린산매급장력단백동원적기인(PTEN)、인유두류병독(HPV)여신강유오이족부녀자궁경병변적상관관계.방법 선취유오이족부녀정상혹염증적자궁경조직표본30례위대조조,궁경병리표본안병변정도급병이학진단분위궁경상피내류양병변(CIN)Ⅰ조、CINⅡ～Ⅲ조각30례、궁경린암조직30례자궁경린암조채용면역조화SP법검측PTEN단백표체,채용취합매련반응(PCR)검측HPV양성솔.결과 PTEN적단백표체솔재정상혹염증적자궁경조직、CIN Ⅰ、CINⅡ～Ⅲ、궁경린암조직중분별위83.3％ 、73.3％、56.7％、23.3％,자궁경린암조양성표체솔명현감소,여전3조비교차이유통계학의의(P＜0.05).4조HPV양성솔분별위43.3％、56.7％、76.7％、93.3％,HPV양성솔화자궁경병변정도정정상관(P＜0.05).결론 신강유오이족부녀자궁경병변조직중PTEN단백수평표체감소,여기궁경병변정부상관관계；시자궁경조직악변적신호.HPV감염여자궁경병변적엄중정도정정상관,HPV감염재자궁경병변적발전과정중기도료겁대적촉진작용,시자궁경암치병인소지일.
Objective To investigate the correlation of phosphataseand tensin homdogy deletedon chromosome ten(PTEN) expression and human papilloma virus (HPV) infection in the cervical lesions of Uygur women.Methods Thirty cases of tissues for each of normal or inflammatory cervical lesion (control),cervical intraepithelial neoplasia(CIN) Ⅰ,CIN Ⅱ and Ⅲ,cervical squamous carcinoma were collected from Uygur women for immunohistochemical detection of PTEN expression.HPV infection was confirmed hy polymerase chain reaction amplification.Results PTEN was expressed in 83.3％,73.3％,56.7％ and 23.3％ of the normal or inflammatory cervical tissues,CIN Ⅰ s,CIN Ⅱ/Ⅲ s and cervical squamous cell carcinoma tissues,respectively.In cervical squamous carcinoma tissue,expression of PTEN was much lower than that of others lesion groups.HPV was positive in 43.3％,56.7％,76.7％ and 93.3％ of the normal or inflammatory cervical tissues,CIN Ⅰ s,CIN Ⅱ / Ⅲ s and cervical squamous cell carcinoma tissues,respectively.HPV infection was positively correlated with the severity of cervical lesions (P ＜ 0.05 ).Conclusion PTEN protein is down-regulated in the cervical lesions of Uygur women and is negatively coreelated with the severity of cervical lesions.HPV infection is negatively correlated with,as one of the promoting factors,the development of cervical transformation.
