CAJ | 학술논문

目的评价异氟醚麻醉对老龄大鼠海马突触体蛋白质组的影响.方法雌性SD大鼠27只,22月龄,体重480～550 g,采用随机数字表法,将其随机分为2组:对照组(C组,n=6)和异氟醚麻醉组(Ⅰ组,n=21).C组吸入含80％氧气的空氧混合气体2h,Ⅰ组吸入3％异氟醚麻醉诱导后,经口明视气管插管,吸入2％异氟醚+80％氧气维持麻醉2h.麻醉结束后24h时,进行Y型迷宫实验测试大鼠认知功能,记录训练次数.以训练次数＞ 75次为判断认知功能低下的标准,将Ⅰ组大鼠分为2组:认知功能低下组(IA组)和认知功能未低下组(IB组).Y型迷宫实验结束后,处死大鼠,取双侧海马组织,提取突触体,进行双向凝胶电泳和质谱分析.结果 Ⅰ组有6只大鼠发生认知功能低下,有13只大鼠未发生认知功能低下.与C组和IB组比较,IA组训练次数增多(P＜0.05)；C组和IB组间比较训练次数差异无统计学意义(P＞0.05).IB组和IA组差异表达的蛋白质有21个,其中11个蛋白质表达上调,10个蛋白质表达下调.C组和IA组差异表达的蛋白质有19个,其中12个蛋白质表达上调,7个蛋白质表达下调.经质谱分析鉴定出31个蛋白质.结论异氟醚麻醉导致老龄大鼠认知功能低下可能与突触部位能量代谢相关蛋白、突触部位的细胞骨架结构及调节蛋白的改变有关.
목적 평개이불미마취대노령대서해마돌촉체단백질조적영향.방법 자성SD대서27지,22월령,체중480～550 g,채용수궤수자표법,장기수궤분위2조:대조조(C조,n=6)화이불미마취조(Ⅰ조,n=21).C조흡입함80％양기적공양혼합기체2h,Ⅰ조흡입3％이불미마취유도후,경구명시기관삽관,흡입2％이불미+80％양기유지마취2h.마취결속후24h시,진행Y형미궁실험측시대서인지공능,기록훈련차수.이훈련차수＞ 75차위판단인지공능저하적표준,장Ⅰ조대서분위2조:인지공능저하조(IA조)화인지공능미저하조(IB조).Y형미궁실험결속후,처사대서,취쌍측해마조직,제취돌촉체,진행쌍향응효전영화질보분석.결과 Ⅰ조유6지대서발생인지공능저하,유13지대서미발생인지공능저하.여C조화IB조비교,IA조훈련차수증다(P＜0.05)；C조화IB조간비교훈련차수차이무통계학의의(P＞0.05).IB조화IA조차이표체적단백질유21개,기중11개단백질표체상조,10개단백질표체하조.C조화IA조차이표체적단백질유19개,기중12개단백질표체상조,7개단백질표체하조.경질보분석감정출31개단백질.결론 이불미마취도치노령대서인지공능저하가능여돌촉부위능량대사상관단백、돌촉부위적세포골가결구급조절단백적개변유관.
Objective To investigate the effects of isoflurane anesthesia on hippocampus synaptosomes proteome in aged rats.Methods Twenty-seven 22- month-old SD rats weighing 480-550 g were randomly divided into 2 groups: control group (group C,n =6) and isoflurane group (group Ⅰ,n =21 ).In group C inhaled mixed gas containing 80％ oxygen for 2 h.In group Ⅰ the animals were endotracheal intubated after induction by 3％ isoflurane and inhaled 2％ isoflurane and 80％ oxygen for 2 h.Cognition function was evaluated by Y-maze at 24 h after anesthesia and the total training times were recorded.The total training times ＞ 75 was defined as cognitive dysfuction.In group Ⅰ the animals were divided into cognitive dysfuction group (group ⅠA) and non-cognitive dysfuction group (group IB) according to the results of Y-maze test.The animals were sacrificed and their hippocampi were removed and synaptosomes were extracted for two-dimensional gel electrophoresis.The different protein spots were analyzed by mass chromatographic analysis.Results Six rats had cognitive dysfuction (group IA) and another thirteen rats had no cognitive dysfuction (group IB).The total training times were significantly higher in group IA than in groups C and IB( P ＜ 0.05).There was no significant difference in the total training times between groups C and IB (P ＞ 0.05).There were 21 (11/10) different protein spots between groups IB and IA,and 19 (12/7) different protein spots between groups C and IA.Thirty-one protein spots were identified by means of MALDI-TOF-MS.Conclusion The cognitive dysfuction after isoflurane anesthesia in aged rats may be related to the changes of energy metabolism protein,cytoskeletal structure and regulatory protein in synapse of hippocampus.