中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2010年
2期
86-89
,共4页
孙艳纯%郑珊%李凯%陈莲%高解春%肖现民
孫豔純%鄭珊%李凱%陳蓮%高解春%肖現民
손염순%정산%리개%진련%고해춘%초현민
神经母细胞瘤%Wnt1蛋白质%β-catenin%CyclinD1
神經母細胞瘤%Wnt1蛋白質%β-catenin%CyclinD1
신경모세포류%Wnt1단백질%β-catenin%CyclinD1
Neuroblastoma%Wnt1 protein%β-catenin%CyclinD1
目的 观察新生儿肾上腺、>2岁小儿肾上腺和神经母细胞瘤组织中经典Wnt信号通路中Wnt1、β-catenin和CyclinD1的表达,探讨Wnt1信号通路调控异常与神经母细胞瘤的相关性.方法 应用免疫组化方法 检测新生儿肾上腺、>2岁小儿肾上腺及各期、各病理类型、化疗前后神经母细胞瘤组织中Wnt1信号通路中Wnt1、β-catenin和CycinD1的表达.结果术前未化疗的肿瘤Wnt1和β-catenin蛋白表达明显强于新生儿肾上腺组织,后者又明显强于>2岁小儿肾上腺(P=0.014),差别均具有高度的统计学意义;Ⅲ～Ⅳ期肿瘤的Wnt1和β-catenin蛋白表达较Ⅰ～Ⅱ期肿瘤高;Ⅲ～Ⅳ期肿瘤中术前未化疗的Wnt1和β-catenin蛋白表达明显高于术前化疗的肿瘤;组织预后不良型(UH)肿瘤Wnt1和β-catenin蛋白表达较组织预后良好型(FH)肿瘤高,差别具有高度的统计学意义.新生儿肾上腺及>2岁小儿肾上腺组织中CyclinD1染色均为阴性,而肿瘤组织中表达阳性,Ⅰ～Ⅱ期肿瘤与Ⅲ～Ⅳ期术前未化疗的肿瘤CyclinD1蛋白表达无差别;Ⅲ～Ⅳ期术前未化疗的肿瘤CyclinD1蛋白表达明显高于术前化疗的肿瘤,UH肿瘤的CyclinD1蛋白表达高于FH肿瘤.结论 Wnt1信号通路对肾上腺细胞分化成熟有一定的调控作用.Wnt1通路异常可能是参与神经母细胞瘤发生和侵袭的重要机制.
目的 觀察新生兒腎上腺、>2歲小兒腎上腺和神經母細胞瘤組織中經典Wnt信號通路中Wnt1、β-catenin和CyclinD1的錶達,探討Wnt1信號通路調控異常與神經母細胞瘤的相關性.方法 應用免疫組化方法 檢測新生兒腎上腺、>2歲小兒腎上腺及各期、各病理類型、化療前後神經母細胞瘤組織中Wnt1信號通路中Wnt1、β-catenin和CycinD1的錶達.結果術前未化療的腫瘤Wnt1和β-catenin蛋白錶達明顯彊于新生兒腎上腺組織,後者又明顯彊于>2歲小兒腎上腺(P=0.014),差彆均具有高度的統計學意義;Ⅲ～Ⅳ期腫瘤的Wnt1和β-catenin蛋白錶達較Ⅰ～Ⅱ期腫瘤高;Ⅲ～Ⅳ期腫瘤中術前未化療的Wnt1和β-catenin蛋白錶達明顯高于術前化療的腫瘤;組織預後不良型(UH)腫瘤Wnt1和β-catenin蛋白錶達較組織預後良好型(FH)腫瘤高,差彆具有高度的統計學意義.新生兒腎上腺及>2歲小兒腎上腺組織中CyclinD1染色均為陰性,而腫瘤組織中錶達暘性,Ⅰ～Ⅱ期腫瘤與Ⅲ～Ⅳ期術前未化療的腫瘤CyclinD1蛋白錶達無差彆;Ⅲ～Ⅳ期術前未化療的腫瘤CyclinD1蛋白錶達明顯高于術前化療的腫瘤,UH腫瘤的CyclinD1蛋白錶達高于FH腫瘤.結論 Wnt1信號通路對腎上腺細胞分化成熟有一定的調控作用.Wnt1通路異常可能是參與神經母細胞瘤髮生和侵襲的重要機製.
목적 관찰신생인신상선、>2세소인신상선화신경모세포류조직중경전Wnt신호통로중Wnt1、β-catenin화CyclinD1적표체,탐토Wnt1신호통로조공이상여신경모세포류적상관성.방법 응용면역조화방법 검측신생인신상선、>2세소인신상선급각기、각병리류형、화료전후신경모세포류조직중Wnt1신호통로중Wnt1、β-catenin화CycinD1적표체.결과술전미화료적종류Wnt1화β-catenin단백표체명현강우신생인신상선조직,후자우명현강우>2세소인신상선(P=0.014),차별균구유고도적통계학의의;Ⅲ～Ⅳ기종류적Wnt1화β-catenin단백표체교Ⅰ～Ⅱ기종류고;Ⅲ～Ⅳ기종류중술전미화료적Wnt1화β-catenin단백표체명현고우술전화료적종류;조직예후불량형(UH)종류Wnt1화β-catenin단백표체교조직예후량호형(FH)종류고,차별구유고도적통계학의의.신생인신상선급>2세소인신상선조직중CyclinD1염색균위음성,이종류조직중표체양성,Ⅰ～Ⅱ기종류여Ⅲ～Ⅳ기술전미화료적종류CyclinD1단백표체무차별;Ⅲ～Ⅳ기술전미화료적종류CyclinD1단백표체명현고우술전화료적종류,UH종류적CyclinD1단백표체고우FH종류.결론 Wnt1신호통로대신상선세포분화성숙유일정적조공작용.Wnt1통로이상가능시삼여신경모세포류발생화침습적중요궤제.
Objective To investigate the role of Wnt1 signaling pathway in the oncogenic trans-formation and invasion of neuroblastomas. Methods Immunohistochemical staining was performed to examine the expressions of Wnt1 signaling proteins including Wnt1,β-catenin and CyclinD1 in adrenal glands and neuroblastoma tissues. Results In the patients without preoperative chemotherapy,the ex-pressions of Wnt1 and β-catenin in neuroblastoma tissues were significantly higher than those of neo-natal adrenal glands. The expressions of Wnt1 and β-catenin in stage Ill-IV neuroblastoma were signif-icantly higher than those of stage Ⅰ-Ⅱ. For tumors of stage Ⅲ-Ⅳ,Wnt1 and β-catenin expressions were significantly decreased in preoperative chemotherapy subgroup than that of non-preoperative chemotherapy subgroup. Wnt1 and β-catenin of unfavorable histology (UH) neuroblastoma were significantly higher than those of favorable histology (FH). In adrenal glands of the neonates,Wnt1 and β-catenin expressed significantly higher than those of the patients older than 2 years of age. The expression of CyclinD1 was negative in the adrenal glands of neonates and patients older than 2 years of age; but it was positive in neuroblastoma tissues. In the patients without preoperative chemotherapy,no difference of CyclinD1 expression was found between stage Ⅰ-Ⅱ and stage Ⅲ-Ⅳ. For tumors of stage Ⅲ-Ⅳ,the CyclinD1 expression was significantly decreased in preoperative chemotherapy subgroup than that of non-preoperative chemotherapy subgroup. The expression of CyclinD1 of UH neuroblastoma was significantly higher than that of FH neuroblastoma. Conclusions Wnt1 signaling pathway plays a role in regulating adrenal gland maturity. The dysregulated Wnt1 signaling may contribute to the oncogenic transformation and invasion of neuroblastoma.
