CAJ | 학술논문

目的探讨采用脂肪瓣延迟术等缺血缺氧的方法,促使脂肪来源的兔间充质干细胞(ASCs)增殖的可能性.方法以兔为动物模型,在一侧腹股沟处形成脂肪瓣.21 d后取出瓣内脂肪组织及对侧正常脂肪组织,消化过筛离心,收集离心后沉淀物中的细胞,用流式细胞仪检测细胞的表面标记,计算细胞CD29、CD44、CD14和CD45的表达率并进行组间比较.结果脂肪瓣延迟术后,瓣内细胞CD29和CD44的表达率增加,分别为74.06%和90.74%,未经处理的对侧脂肪组织分别为62.88%和77.54%,差异有统计学意义(P<0.05);瓣内细胞CD14和CD45的表达率减少,分别为57.66%和4.84%,未经处理的对侧脂肪组织分别为72.10%和75.82%,差异有统计学意义(P<0.05或P<0.01).结论脂肪瓣延迟术等缺血缺氧的方法,可促进ASCs的增殖,是用瓣内组织移植后取得更好效果的机制之一;对脂肪组织先进行缺血预处理再移植,可能为一个临床脂肪移植的新方法.
목적 탐토채용지방판연지술등결혈결양적방법,촉사지방래원적토간충질간세포(ASCs)증식적가능성.방법 이토위동물모형,재일측복고구처형성지방판.21 d후취출판내지방조직급대측정상지방조직,소화과사리심,수집리심후침정물중적세포,용류식세포의검측세포적표면표기,계산세포CD29、CD44、CD14화CD45적표체솔병진행조간비교.결과 지방판연지술후,판내세포CD29화CD44적표체솔증가,분별위74.06%화90.74%,미경처리적대측지방조직분별위62.88%화77.54%,차이유통계학의의(P<0.05);판내세포CD14화CD45적표체솔감소,분별위57.66%화4.84%,미경처리적대측지방조직분별위72.10%화75.82%,차이유통계학의의(P<0.05혹P<0.01).결론 지방판연지술등결혈결양적방법,가촉진ASCs적증식,시용판내조직이식후취득경호효과적궤제지일;대지방조직선진행결혈예처리재이식,가능위일개림상지방이식적신방법.
Objective To investigate the possibility to enhance the proliferation of adipose-derived stem cells (ASCs) in a delayed fat flap in rabbits. Methods A delayed fat flap was formed in one side of inguinal region of a rabbit. 21 days after operation, the fat tissues at the delayed flaps and at the unoperated side were harvested and digested with 0.25% collageusse and sieved. The cell suspensions were centrifuged. The cells were obtained from tissue precipitate after centrifugation. The expression rates of the surface marker (CD29 CD44,CD14 and CD45) were measured by FCM and compared between the experimental and control groups. Results Expression rates of CD29 and CD44 were higher in the delayed fat flap (74.06% and 90.74%) than in the eontralateral fat tissue (62.88% and 77.54%, P < 0.05), while those of CD14 and CD45 were lower in the delayed fat flap (57.66% and 4.84%) than in the eontralateral fat tissue (72.10% and 75.82%, P < 0.05 and P < 0.01). Conclusions Tissue hypoxic isehemia such as fat tissue in a delayed fat flap can promote proliferation of ASCs. It indicates that tissue in the delayed flap may be transplanted with better survival rate. The ischemia pretreatement of fat tissue may become a new method for fat transplantion.