CAJ | 학술논문

从黄瓜(Cucumis sativus L.)叶片中分离出只含有一种亚基(27 kD)的LHC-Ⅱ复合物.采用batch方法获得了其二维晶体,大小为0.7 μm×1.0μm,衍射能力达30 A.负染样品的二维投影结果表明,该晶体为p3对称性,晶胞参数为15.4 nm×15.4 nm,不同于以往报道的菠菜(Spinacia oleracea L.)或豌豆(Pisum satium L.)LHC-Ⅱ晶体,为另外一种晶型.采用tomography技术,收集了0°～-55°系列倾斜照片,进行三维重构.LHC-Ⅱ复合物是由6个单体组成的六元环,相邻2个单体分别从膜的两侧插膜,方向相反,在膜区靠疏水-疏水相互作用成二聚体,3个相同的二聚体相互连接成六元环.
종황과(Cucumis sativus L.)협편중분리출지함유일충아기(27 kD)적LHC-Ⅱ복합물.채용batch방법획득료기이유정체,대소위0.7 μm×1.0μm,연사능력체30 A.부염양품적이유투영결과표명,해정체위p3대칭성,정포삼수위15.4 nm×15.4 nm,불동우이왕보도적파채(Spinacia oleracea L.)혹완두(Pisum satium L.)LHC-Ⅱ정체,위령외일충정형.채용tomography기술,수집료0°～-55°계렬경사조편,진행삼유중구.LHC-Ⅱ복합물시유6개단체조성적륙원배,상린2개단체분별종막적량측삽막,방향상반,재막구고소수-소수상호작용성이취체,3개상동적이취체상호련접성륙원배.
Cucumber (Cucumis sativus L.) LHC- Ⅱ complex, which consists of only one subunit (27 kD),was isolated and purified. 2-D crystallization was performed by batch method. The crystal is 0.7 μm × 1.0μm, and diffracts to 30 A. The projection map of the negatively stained two-dimensional crystal of LHC-Ⅱcomplex shows that the crystal has p3 symmetry, lattice constant 15.4 nm × 15.4 nm, which is different fromthe LHC- Ⅱ of spinach (Spinacia oleracea L.) and pea (Pisum satium L.). A continuous tomographic tilt se-ties, containing 12 projections from the two-dimensional crystal was subjected to 3-D reconstruction. The 3-Dmodel represents that LHC-Ⅱ complex consists of 6 monomers. These trimer and dimer interactions build upthe six-member ting.