CAJ | 학술논문

目的:研究睾丸Sertoli细胞感染后,TLR发挥免疫调节功能的情况.方法:正常大鼠Sertoli细胞表达Toll样受体的情况以及用溶脲脲原体(Ureaplasma Urealyticum,UU)体外感染Sertoli细胞后,分别在12、24、36小时时间段比较感染组与对照组之间Toll样受体表达变化情况.结果:正常大鼠Sertoli细胞表达TLR2-8,低表达TLR9和10,未检出TLR1和5;与对照组相比,体外感染处理后TLR2、6表达增加.结论:Toll样受体的激活参与Sertoli细胞对炎症的免疫调节,两者具有一定的联系.
목적:연구고환Sertoli세포감염후,TLR발휘면역조절공능적정황.방법:정상대서Sertoli세포표체Toll양수체적정황이급용용뇨뇨원체(Ureaplasma Urealyticum,UU)체외감염Sertoli세포후,분별재12、24、36소시시간단비교감염조여대조조지간Toll양수체표체변화정황.결과:정상대서Sertoli세포표체TLR2-8,저표체TLR9화10,미검출TLR1화5;여대조조상비,체외감염처리후TLR2、6표체증가.결론:Toll양수체적격활삼여Sertoli세포대염증적면역조절,량자구유일정적련계.
Objective:To investigate the relationship between Toll-like receptor and the immune regulation about inflammation by Sertoli cell in vitro.Methods:Here we examined the expression and potential functions of TLR family in rat Sertoli cells.Using our well-characterized urealyticum(UU) induced model we tested the expression changes of TLR2 and TLR6 at 12~(th),24~(th),36~(th) hours after UU infection in vitro.Results:We demonstrated that TLR2-8 are highly expressed;TLR9 and TLR10 are expressed at relatively low level;the expression of TLR1 and TLR5 are not detected in normal rat Sertoli cells.Comparing with control group,Sertoli cells express more TLR2 and TLR6 after infected by UU.Conclusion:There is some relationship between the activation of TLRs and the immune regulation about inflammation by Sertoli cell.