CAJ | 학술논문

氨基硅烷化超顺磁纳米颗粒的制备及其在小鼠体内的分布
안기규완화초순자납미과립적제비급기재소서체내적분포
Preparation of Amido Silanization Modified SPION and Its in Vivo Behavior on KM Mice

万方数据

中国医学影像学杂志中國醫學影像學雜誌 중국의학영상학잡지
CHINESE JOURNAL OF MEDICAL IMAGING
2010年 2期 101-105 ,共5页

贺娟%邓均%郑峻松%黄辉%方立超%李艳%程平%蒋丽莉%张楠%李兆刚%彭思洋

하연%산균%정준송%황휘%방립초%리염%정평%장려리%장남%리조강%팽사양

硅烷类%金属钠米粒子,超顺磁%组织分布硅烷類%金屬鈉米粒子,超順磁%組織分佈
규완류%금속납미입자,초순자%조직분포
silanes%metal nanoparticles,superparamagnetic%tissue distribution

目的:制备氨基硅烷化的超顺磁纳米颗粒(superparamagnetic iron oxide nanoparticles,SPION),并检测其物理性质以及在小鼠体内的自然分布,从而探讨该系统在小鼠体内分布的规律.材料和方法:共沉淀一步法制备葡聚糖外包的四氧化三铁纳米颗粒,然后在通氮、60℃、强力机械搅拌的条件下加入AEAPS,得到氨基硅烷化的SPION.采用透射电镜测量其大小及分布,用气相色谱法检测AEAPS与葡聚糖-SPION的结合率,用原子吸收分光光度计研究其在小鼠体内的分布情况.结果:所得样品核心为四氧化三铁晶体,核心粒径不超过25nm,氨基硅烷化超顺磁性纳米颗粒直径不超过30nm,AEAPS与葡聚糖-SPION的结合率为91.15%,自然状态下经尾静脉给药后氨基硅烷化超顺磁性纳米颗粒主要被肝脏和脾脏摄取,但不被肾脏摄取.结论:制备的样品具有粒径小,分散性好,超顺磁性等优点,同时可以经血液循环系统到达肝脏、脾脏、心脏、肺.
목적:제비안기규완화적초순자납미과립(superparamagnetic iron oxide nanoparticles,SPION),병검측기물이성질이급재소서체내적자연분포,종이탐토해계통재소서체내분포적규률.재료화방법:공침정일보법제비포취당외포적사양화삼철납미과립,연후재통담、60℃、강력궤계교반적조건하가입AEAPS,득도안기규완화적SPION.채용투사전경측량기대소급분포,용기상색보법검측AEAPS여포취당-SPION적결합솔,용원자흡수분광광도계연구기재소서체내적분포정황.결과:소득양품핵심위사양화삼철정체,핵심립경불초과25nm,안기규완화초순자성납미과립직경불초과30nm,AEAPS여포취당-SPION적결합솔위91.15%,자연상태하경미정맥급약후안기규완화초순자성납미과립주요피간장화비장섭취,단불피신장섭취.결론:제비적양품구유립경소,분산성호,초순자성등우점,동시가이경혈액순배계통도체간장、비장、심장、폐.
Purpose To explore the method of preparation of Amido Silanization modified SPION ( superparamagnetic iron oxide nanoparticles) and to examine its physical properties and its in vivo behavior on KM mice. Materials and Methods Dextran-modified SPION was obtained by "one step" co-precipitation method. Then, AEAPS was added to the resultant Dextran-SPION under the condition of N_2 saturation and high-speed mechanical stirring at 60℃. The size of the nanoparticles was measured by transmission electron microscope; The binding ratio of AEAPS was measured by gas chromatography; The in vivo distribution was measured by atomic absorption spectrophotometer. Results The AEAPS/Dextran-modified SPION nanoparticles had core-shell diameter less than 30nm with magnetic core of less than 25nm in diameter; The binding ratio of AEAPS was 91.15%; Under natural conditions most of the AEAPS/Dextran-modified SPION was taken up by liver and spleen after the administration via tail vain, but was not taken up by kidney. Conclusion The AEAPS/Dextran-modified SPION possess characteristics of small size, good dispersion superparamagnetic response, as well as by the blood circulatory system to reach the liver, spleen, heart, lungs.