国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2012年
6期
850-852
,共3页
茶碱缓释片%茶碱%反相高效液相色谱法%含量测定
茶堿緩釋片%茶堿%反相高效液相色譜法%含量測定
다감완석편%다감%반상고효액상색보법%함량측정
Theophylline sustained-release tablets%Theophylline%RP HPLC%Determination of the contents
目的 建立茶碱缓释片中茶碱的含量测定方法.方法 采用反相高效液相色谱法,Hypersil C18柱(5μm,150 mm×4.6 mm),以甲醇-水(30∶70)为流动相,流速为1.0 ml/min;检测波长203 nm；柱温为室温,进样量为20 μl.结果 茶碱在4～36 μg/ml浓度范围内线性关系良好,r=0.999 9；平均回收率为99.2％,方法精密度(RSD)为0.29％(n=5).结论 反相高效液相色谱法可用于茶碱缓释片中茶碱的含量测定,以控制茶碱缓释片的质量.
目的 建立茶堿緩釋片中茶堿的含量測定方法.方法 採用反相高效液相色譜法,Hypersil C18柱(5μm,150 mm×4.6 mm),以甲醇-水(30∶70)為流動相,流速為1.0 ml/min;檢測波長203 nm；柱溫為室溫,進樣量為20 μl.結果 茶堿在4～36 μg/ml濃度範圍內線性關繫良好,r=0.999 9；平均迴收率為99.2％,方法精密度(RSD)為0.29％(n=5).結論 反相高效液相色譜法可用于茶堿緩釋片中茶堿的含量測定,以控製茶堿緩釋片的質量.
목적 건립다감완석편중다감적함량측정방법.방법 채용반상고효액상색보법,Hypersil C18주(5μm,150 mm×4.6 mm),이갑순-수(30∶70)위류동상,류속위1.0 ml/min;검측파장203 nm；주온위실온,진양량위20 μl.결과 다감재4～36 μg/ml농도범위내선성관계량호,r=0.999 9；평균회수솔위99.2％,방법정밀도(RSD)위0.29％(n=5).결론 반상고효액상색보법가용우다감완석편중다감적함량측정,이공제다감완석편적질량.
Objective To establish a reversed-phased high performance liquid chromatography (RP-HPLC) method for the determination of Theophylline sustained release tablets.Methods By using RP-HPLC,a Hypersil C18 column was used (5 μ m,150 mm× 4.6 mm),with the mobile phase of methanol-water(30∶70),the flowing rate was 1.0 ml/min,and the detection wavelength was 203 nm in the room temperature.Results Theophylline was linear over the range of 4 ～ 36 μ g/ml(r=0.999 9,n=5).The average recovery was 99.2％ (RSD=0.29％, n=5).Conclusion It's a feasible method for the determination of theophylline so as to control the quality of theophylline sustained release tablets.
