国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2010年
1期
13-16,20,后插2
,共6页
徐新宇%翟文倩%王国林%田心
徐新宇%翟文倩%王國林%田心
서신우%적문천%왕국림%전심
丙泊酚%大鼠%空间关联记忆能力%Morris水迷宫%空间记忆能力
丙泊酚%大鼠%空間關聯記憶能力%Morris水迷宮%空間記憶能力
병박분%대서%공간관련기억능력%Morris수미궁%공간기억능력
Propofol%Rats%Spatial associative memory capacity%Morris water maze%Spatial memory capacity
目的 研究注射临床低剂量和高剂量的丙泊酚对大鼠空间关联记忆能力的影响.方法 将30只大鼠随机分成3组:对照组、临床低剂量0.1 mg/(kg·min)组和临床高剂量0.5 mg/(kg·min)组.在注射丙泊酚1 d后进行水迷宫实验,包括4 d的定位航行和1 d的空间探索实验.在定位航行实验中分别对3组大鼠进行Ⅰ、Ⅱ和Ⅲ象限入水的训练,并记录3组大鼠的逃避潜伏期、游泳路程和游泳速度.在空间探索实验巾将大鼠从未训练的Ⅳ象限入水,分别记录平台象限游泳时间、第一环点数以及总得分.结果 在第1,2天的定位航行实验中,低剂量组与对照组大鼠的逃避潜伏期没有统计学差异(P>0.05);高剂量组逃避潜伏期比对照组显著延长(P<0.05).第3,4天的定位航行实验中,3组大鼠之间均未见差异(P>0.05).在空间探索实验中,从未训练的Ⅳ象限入水时,对照组、低剂量组和高剂量组大鼠的总得分分别为(2 509.36±190.72)、(2 378.55±210.69)和(1 954.85±174.20).低剂量组和对照组没有显著性差异(P>0.05);高剂量组与对照组存在显著性差异(P<0.05),即高剂量组比对照组总得分低.结论 注射低剂量的丙泊酚不影响大鼠的空间关联记忆和空间记忆能力;高剂量丙泊酚在注射后前2 d内降低大鼠的空间记忆能力,但2 d后对大鼠的空间记忆能力没有影响,说明高剂量的丙泊酚降低了大鼠的空间关联记忆能力.
目的 研究註射臨床低劑量和高劑量的丙泊酚對大鼠空間關聯記憶能力的影響.方法 將30隻大鼠隨機分成3組:對照組、臨床低劑量0.1 mg/(kg·min)組和臨床高劑量0.5 mg/(kg·min)組.在註射丙泊酚1 d後進行水迷宮實驗,包括4 d的定位航行和1 d的空間探索實驗.在定位航行實驗中分彆對3組大鼠進行Ⅰ、Ⅱ和Ⅲ象限入水的訓練,併記錄3組大鼠的逃避潛伏期、遊泳路程和遊泳速度.在空間探索實驗巾將大鼠從未訓練的Ⅳ象限入水,分彆記錄平檯象限遊泳時間、第一環點數以及總得分.結果 在第1,2天的定位航行實驗中,低劑量組與對照組大鼠的逃避潛伏期沒有統計學差異(P>0.05);高劑量組逃避潛伏期比對照組顯著延長(P<0.05).第3,4天的定位航行實驗中,3組大鼠之間均未見差異(P>0.05).在空間探索實驗中,從未訓練的Ⅳ象限入水時,對照組、低劑量組和高劑量組大鼠的總得分分彆為(2 509.36±190.72)、(2 378.55±210.69)和(1 954.85±174.20).低劑量組和對照組沒有顯著性差異(P>0.05);高劑量組與對照組存在顯著性差異(P<0.05),即高劑量組比對照組總得分低.結論 註射低劑量的丙泊酚不影響大鼠的空間關聯記憶和空間記憶能力;高劑量丙泊酚在註射後前2 d內降低大鼠的空間記憶能力,但2 d後對大鼠的空間記憶能力沒有影響,說明高劑量的丙泊酚降低瞭大鼠的空間關聯記憶能力.
목적 연구주사림상저제량화고제량적병박분대대서공간관련기억능력적영향.방법 장30지대서수궤분성3조:대조조、림상저제량0.1 mg/(kg·min)조화림상고제량0.5 mg/(kg·min)조.재주사병박분1 d후진행수미궁실험,포괄4 d적정위항행화1 d적공간탐색실험.재정위항행실험중분별대3조대서진행Ⅰ、Ⅱ화Ⅲ상한입수적훈련,병기록3조대서적도피잠복기、유영로정화유영속도.재공간탐색실험건장대서종미훈련적Ⅳ상한입수,분별기록평태상한유영시간、제일배점수이급총득분.결과 재제1,2천적정위항행실험중,저제량조여대조조대서적도피잠복기몰유통계학차이(P>0.05);고제량조도피잠복기비대조조현저연장(P<0.05).제3,4천적정위항행실험중,3조대서지간균미견차이(P>0.05).재공간탐색실험중,종미훈련적Ⅳ상한입수시,대조조、저제량조화고제량조대서적총득분분별위(2 509.36±190.72)、(2 378.55±210.69)화(1 954.85±174.20).저제량조화대조조몰유현저성차이(P>0.05);고제량조여대조조존재현저성차이(P<0.05),즉고제량조비대조조총득분저.결론 주사저제량적병박분불영향대서적공간관련기억화공간기억능력;고제량병박분재주사후전2 d내강저대서적공간기억능력,단2 d후대대서적공간기억능력몰유영향,설명고제량적병박분강저료대서적공간관련기억능력.
Objective To study the clinical effects of low-dose and high-dose injection of propefol on spatial associative memory capacity in rats. Methods 30 rats were randomly divided into 3 groups: control, clin-ical low-dose propofol (0.1 mg/kg·min) group and clinical high-dose propofol (0.5 mg/kg·min) group. The experi-ments including 4-days place swimming and 1-day space exploration began from the day after injection. In place swimming group, rats were trained in morris water maze from Ⅰ,Ⅱ,Ⅲ quadrant. Latency period, swimming length and swimming velocity were recorded. In space exploration group, untrained rats were put into the maze from the Ⅳ quadrant and swimming time, first circling point value and total scores were recorded. Results At the first two days of place swimming test there were no differences between the control group and the low-dose group in la-tency, swimming length and swimming velocity(P<0.05); while high-dose group's latency period and swimming length were obviously longer than that of control(P<0.05). At the second two days there were no differences among the three groups(P<0.05). In the space exploration test, total scores of control, low-dose group and high-dose group were: 2 509.36±190.72, 2 378.55±210.69 and 1 954.85±174.20, respectively. There was no difference be-tween low-dose group and the control group(P<0.05). Total scores of the high-dose group were much lower than that of control. Conclusion The clinical low-dose propofol injection has no effects on the spatial associative memory capacity and spatial memory capacity of rats. The clinical high-dose propofol injection affects the rats' spatial memory capacity during the first two days but has no effects after two days, while the clinical high-dose propofol injection reduces the rats' spatial associative memory capacity.
