CAJ | 학술논문

目的利用产气荚膜梭菌qPCR法检测A型产气荚膜梭菌(Clostridium perfringens type A)感染小鼠的后肢的气性坏疽动物模型和一例阳性病例,以验证此法在早期诊断优势.方法小鼠随机分成4组,10只/组分别肌肉注射3.5×109、3.5×108和3.5×107cfu/ml浓度的A型产气荚膜梭菌(ATCC13124)菌液0.1 ml,空白对照组肌肉注射生理盐水0.1 ml,建立小鼠感染的动物模型,利用产气荚膜梭菌qPCR方法对动物模型及一例病例进行检测分析.结果不同浓度菌液组(3.5×109、3.5×108、3.5×107cfu/ml实验组)和对照组在肌肉注射72 h内的死亡率为:90%、70%、10%和0;各组平均存活时间(hh:mm)依次为:20∶43±11∶12、37∶24±25∶39、68∶36±10∶45和72∶00±0∶00,各组荧光定量Ct值均值为:21.21±2.69、28.45±2.74、32.49±2.87和0.00±0.00,组间比较差异均有统计学意义(P＜0.05).利用该法检测病者两处分泌物,Ct值为30.67、30.44.结论利用产气荚膜梭菌qPCR法成功检测出未发病的小鼠中的产气荚膜梭菌,并成功的应用于一例病例中.
목적 이용산기협막사균qPCR법검측A형산기협막사균(Clostridium perfringens type A)감염소서적후지적기성배저동물모형화일례양성병례,이험증차법재조기진단우세.방법 소서수궤분성4조,10지/조분별기육주사3.5×109、3.5×108화3.5×107cfu/ml농도적A형산기협막사균(ATCC13124)균액0.1 ml,공백대조조기육주사생리염수0.1 ml,건립소서감염적동물모형,이용산기협막사균qPCR방법 대동물모형급일례병례진행검측분석.결과 불동농도균액조(3.5×109、3.5×108、3.5×107cfu/ml실험조)화대조조재기육주사72 h내적사망솔위:90%、70%、10%화0;각조평균존활시간(hh:mm)의차위:20∶43±11∶12、37∶24±25∶39、68∶36±10∶45화72∶00±0∶00,각조형광정량Ct치균치위:21.21±2.69、28.45±2.74、32.49±2.87화0.00±0.00,조간비교차이균유통계학의의(P＜0.05).이용해법검측병자량처분비물,Ct치위30.67、30.44.결론 이용산기협막사균qPCR법성공검측출미발병적소서중적산기협막사균,병성공적응용우일례병례중.
Objective To detect of clostridium perfringens by qPCR in mouse models and a clinical case in order to offer early diagnosis.Methods 40 Kunming mice were randomly grouped and intramuscular injected clostridium perfringens type A in leg 0.1 ml(3.5 × 109cfu/ml or 3.5 × 108cfu/ml or 3.5× 107cfu/ml,diluted with saline),while control group was injected with 9% sodium chloride 0.1ml.The mouse models and a clinical case were detected by qPCR.Results The death rate of 3.5 × 109,3.5 × 108,3.5 × 107cfu/ml and the blank group were 90%,70%,10% and 0% after intramuscular injection for 72 h spectively.The mean Ct values among these groups were 21.21 ±2.69,28.45 ±2.74,32.49 ±2.87 and 0.00 ± 0.00(P ＜ 0.05).The Ct values of the patient were 30.67 and 30.44.Conclusions Cclostridium perfringens could be successful identified with qPCR in mouse models when the mice still did not show any symptoms.