中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2002年
3期
433-438
,共6页
李皓%尹鸿操%张华%曹心宜%王宗立%佘铭鹏
李皓%尹鴻操%張華%曹心宜%王宗立%佘銘鵬
리호%윤홍조%장화%조심의%왕종립%사명붕
血管紧张素Ⅱ NF-κB 内皮细胞 PDGF-B
血管緊張素Ⅱ NF-κB 內皮細胞 PDGF-B
혈관긴장소Ⅱ NF-κB 내피세포 PDGF-B
angiotensin Ⅱ * nuclear factor-kappa B * endothelial cell * PDGF-B
目的 研究血管紧张素Ⅱ对ECV304细胞中转录因子NF-κB的作用和对PDGF-B 基因表达的影响.方法 采用电泳迁移率移动分析法(EMSA)和免疫组化方法,包括共聚焦显微镜及金颗粒标记免疫电镜技术；荧光素酶报告基因与变异型激酶质粒共转染的方法及Northern 印迹法研究血管紧张素Ⅱ激活NF-κB的信号传递路径和检测了血管紧张素Ⅱ刺激前后PDGF-B mRNA的表达水平.结果 血管紧张素Ⅱ刺激后,在ECV304细胞内有NF-κB的激活及核易位过程,应用免疫荧光共聚焦显微镜,免疫电镜及Northern 印迹等方法均可观察到PDGF-B或其基因表达增高.变异型激酶质粒IKKα-KM,IKKβ-KM 及 NIK-KM 可抑制经AngⅡ刺激的转染细胞内与NF-κB启动相连的荧光素酶的表达.结论 AngⅡ可激活胞浆内NF-κB并出现核易位,激酶NIK、IKKα和IKKβ参与了此信号传递路径.血管紧张素Ⅱ刺激后PDGF-B链mRNA水平增高.
目的 研究血管緊張素Ⅱ對ECV304細胞中轉錄因子NF-κB的作用和對PDGF-B 基因錶達的影響.方法 採用電泳遷移率移動分析法(EMSA)和免疫組化方法,包括共聚焦顯微鏡及金顆粒標記免疫電鏡技術；熒光素酶報告基因與變異型激酶質粒共轉染的方法及Northern 印跡法研究血管緊張素Ⅱ激活NF-κB的信號傳遞路徑和檢測瞭血管緊張素Ⅱ刺激前後PDGF-B mRNA的錶達水平.結果 血管緊張素Ⅱ刺激後,在ECV304細胞內有NF-κB的激活及覈易位過程,應用免疫熒光共聚焦顯微鏡,免疫電鏡及Northern 印跡等方法均可觀察到PDGF-B或其基因錶達增高.變異型激酶質粒IKKα-KM,IKKβ-KM 及 NIK-KM 可抑製經AngⅡ刺激的轉染細胞內與NF-κB啟動相連的熒光素酶的錶達.結論 AngⅡ可激活胞漿內NF-κB併齣現覈易位,激酶NIK、IKKα和IKKβ參與瞭此信號傳遞路徑.血管緊張素Ⅱ刺激後PDGF-B鏈mRNA水平增高.
목적 연구혈관긴장소Ⅱ대ECV304세포중전록인자NF-κB적작용화대PDGF-B 기인표체적영향.방법 채용전영천이솔이동분석법(EMSA)화면역조화방법,포괄공취초현미경급금과립표기면역전경기술；형광소매보고기인여변이형격매질립공전염적방법급Northern 인적법연구혈관긴장소Ⅱ격활NF-κB적신호전체로경화검측료혈관긴장소Ⅱ자격전후PDGF-B mRNA적표체수평.결과 혈관긴장소Ⅱ자격후,재ECV304세포내유NF-κB적격활급핵역위과정,응용면역형광공취초현미경,면역전경급Northern 인적등방법균가관찰도PDGF-B혹기기인표체증고.변이형격매질립IKKα-KM,IKKβ-KM 급 NIK-KM 가억제경AngⅡ자격적전염세포내여NF-κB계동상련적형광소매적표체.결론 AngⅡ가격활포장내NF-κB병출현핵역위,격매NIK、IKKα화IKKβ삼여료차신호전체로경.혈관긴장소Ⅱ자격후PDGF-B련mRNA수평증고.
Objective To examine the effect of angiotensin Ⅱ (Ang Ⅱ) on nuclear factor-kappa B (NF-κB) activation in human endothelial cell line ECV304 and the molecular mechanism by which Ang Ⅱ activates NF-κB. Methods ECV304 cells were transiently cotransfected with an NF-κB/ uciferase reporter gene and inactive NF-κB-inducing kinase (NIK), IκB kinase α (IKKα), IκB kinase β (IKKβ) mutants or vectors, respectively. The effect on NF-κB was detected by using an electrophoretic mobility shift assay (EMSA) and overexpression of the mutants enabled blocking of reporter gene activation induced by Ang Ⅱ. With immunofluorescence and immuno-electronic microscope techniques, including confocal microscopy and gold particle labeled electronic microscopy, definite cytoplasmic-to-nuclear translocations of NF-κB activation were detected using subunits p50 and p65 induced by Ang Ⅱ. Results The translocation of p50 in nuclei was highly remarkable 2 hours after Ang Ⅱ stimulation, and the activity was somewhat reduced 6 hours after stimulation to the 18th hour. Northern blot also showed PDGF-B mRNA increased by stimulation of Ang Ⅱ for 18 hours. Conclusion Ang Ⅱ is effective in stimulating NF-κB activation through a pathway dependent on NIK, IKKα and IKKβ, and induces PDGF-B transcription in the endothelial cell line, ECV304.
