CAJ | 학술논문

目的观察电穿孔法转染靶向雄激素受体(AR)的小干扰RNA(siRNA)对裸鼠人膀胱癌移植瘤生长的影响.方法建立人膀胱癌细胞124的荷瘤裸鼠模型,于肿瘤直径0.5 cm大小时,瘤体接受AR-siRNA的电转染治疗,转染无效序列siRNA为阴性对照组,瘤体未受电穿击为空白对照组.观察裸鼠肿瘤生长;4周后处死,绘制肿瘤生长曲线;肿瘤组织石蜡包埋,常规切片,TUNEL法检测移植瘤凋亡.结果电转染AR-siRNA后瘤体组织AR基因的表达显著被抑制,也能明显抑制裸鼠移植瘤的生长;TUNEL检测凋亡率为(13.1±6.9)%,显著高于阴性对照组(P＜0.01).结论电穿孔法靶向AR的siRNA可有效阻断种植瘤内AR表达,阻断雄激素受体途经信号传导,进而诱导细胞凋亡,能明显抑制人膀胱癌裸鼠皮下移植瘤的生长.
목적 관찰전천공법전염파향웅격소수체(AR)적소간우RNA(siRNA)대라서인방광암이식류생장적영향.방법 건립인방광암세포124적하류라서모형,우종류직경0.5 cm대소시,류체접수AR-siRNA적전전염치료,전염무효서렬siRNA위음성대조조,류체미수전천격위공백대조조.관찰라서종류생장;4주후처사,회제종류생장곡선;종류조직석사포매,상규절편,TUNEL법검측이식류조망.결과 전전염AR-siRNA후류체조직AR기인적표체현저피억제,야능명현억제라서이식류적생장;TUNEL검측조망솔위(13.1±6.9)%,현저고우음성대조조(P＜0.01).결론 전천공법파향AR적siRNA가유효조단충식류내AR표체,조단웅격소수체도경신호전도,진이유도세포조망,능명현억제인방광암라서피하이식류적생장.
Objective To investigate the antitumor efficacy of small interfering RNA (siRNA)mediated inhibition of androgen receptor (AR) gene expression in T24 bladder tumor xenografts using electroporation. Methods Athymic mouse human bladder cancer transplantation tumor model was established by injecting T24 cells subcutaneously. When tumor diameter was exceeded 0. 5 cm, AR-siRNA was deliered into tumor xenografts by electroporation. The cells with nonspecific siRNA delivery and un-treatment served as control groups. Tumor volumes were measured weekly. The animals were sacrificed after the treatments, and the histological changes of xenografts were observed by Hematoxylin and Eosin ( HE) staining and TUNEL assay. Results The athymic mouse exnograft tumor model was established successfully.After AR-siRNA treatment, tumor growth was inhibited as compared with the controls. The number of TUNEL-positive cells was significantly increased in AR-siRNA group as compared with the nonspecific siRNA group ( P ＜ 0. 01). Conclusion siRNA targeting AR gene could inhibit obviously the growth of athymic mouse human T24 bladder carcinoma transplantation tumor by inducing apoptosis.