CAJ | 학술논문

目的检测胆管癌和癌旁0.5cm胆管组织及手术切缘的正常胆管组织增殖细胞核抗原(PCNA)蛋白及mRNA的表达,探讨其在胆管癌发生发展中的作用.方法应用免疫组织化学过氧化物酶标记链霉卵白素法(SP)检测42例胆管癌组织及20例正常组织中PCNA蛋白的表达,同时应用逆转录-聚合酶链反应(RT-PCR)技术检测42例术中所取的新鲜的胆管癌、17例同个体癌旁胆管黏膜和20例正常胆管组织中的PCNA mRNA表达,并与临床资料进行相关分析.结果正常胆管组织、癌旁组织、胆管癌组织中PCNA mRNA相对表达量分别0.5605±0.0331,0.5692±0.0408,0.6303±0.0773,PCNA mRNA在3组之间表达呈上升趋势(P<0.05),PCNA蛋白表达趋势同其相对应的基因表达趋势一致,即PCNA mRNA在胆管癌组织中高表达(P<0.05).结论 PC-NA基因转录和蛋白可能参与了胆管癌的发生发展过程.
목적 검측담관암화암방0.5cm담관조직급수술절연적정상담관조직증식세포핵항원(PCNA)단백급mRNA적표체,탐토기재담관암발생발전중적작용.방법 응용면역조직화학과양화물매표기련매란백소법(SP)검측42례담관암조직급20례정상조직중PCNA단백적표체,동시응용역전록-취합매련반응(RT-PCR)기술검측42례술중소취적신선적담관암、17례동개체암방담관점막화20례정상담관조직중적PCNA mRNA표체,병여림상자료진행상관분석.결과 정상담관조직、암방조직、담관암조직중PCNA mRNA상대표체량분별0.5605±0.0331,0.5692±0.0408,0.6303±0.0773,PCNA mRNA재3조지간표체정상승추세(P<0.05),PCNA단백표체추세동기상대응적기인표체추세일치,즉PCNA mRNA재담관암조직중고표체(P<0.05).결론 PC-NA기인전록화단백가능삼여료담관암적발생발전과정.
Objective To detect the expression of proliferating cell nuclear antigen (PCNA) protein and gene in eholangionearcinoma and to elucidate its role. Methods The express of PCNA protein was detected by Immunohistochemical technique (SP) in 42 cases of eholangionearcinoma and 20 cases normal bile duct,and the express of PCNA gene was detected in 42 cases of eholangionearcinoma, adjacent to eholangionearcinoma and normal bile duct by reverse transcription-polymerase chain reaction (RT-PCR). The correlation was analysed statistically between the express of PCNA protein and mRNA and clinicopatho-logical parameters in eholangionearcinoma. Results The express of PCNA mRNA in normal bile duct, adjacent to eholangionearcinoma,eholangionearcinoma respectively was 0. 5605 ±0. 0331,0. 5692 ± 0.0408, 0. 6303 ±0.0773,the express of PCNA mRNA was higher in eholangionearcinoma than the others (P< 0.05). the protein expression of PCNA was higher in eholangionearcinoma than normal bile duct tissue (P<0.05). Conclusion PCNA gene transcription and protein expression is mostly like involved in the proliferation and malignant transformation of bile epithelial and then malignant progression of bile duct carcinomas. The detection of PCNA expression may elucidate the carcinogenesis of bile duct.