CAJ | 학술논문

目的研究异基因干细胞移植免疫抑制剂的剂量与多瘤病毒载量的关系,预防移植后出血性膀胱炎的发生.方法收集122例干细胞移植患者的血液和同期尿液及1例移植后连续8个月外周血和同期尿液,提取尿液DNA,合成多瘤病毒BKV基因引物探针,荧光定量PCR法检测病毒基因载量和荧光偏振免疫法测定血液环胞素A浓度,动态观察分析二者之间的关系.结果当血液环胞素A浓度高于86～105 ng/ml,尿液BKV-DNA病毒检测阳性,尿液BKV-DNA病毒载量随着血液环胞素A浓度而改变,二者呈线性关系.结论当机体处于免疫抑制状态时BKV病毒-DNA的载量与使用免疫抑制剂的剂量有关,增加了移植后诱发出血性膀胱炎的危险性.
목적 연구이기인간세포이식면역억제제적제량여다류병독재량적관계,예방이식후출혈성방광염적발생.방법 수집122례간세포이식환자적혈액화동기뇨액급1례이식후련속8개월외주혈화동기뇨액,제취뇨액DNA,합성다류병독BKV기인인물탐침,형광정량PCR법검측병독기인재량화형광편진면역법측정혈액배포소A농도,동태관찰분석이자지간적관계.결과당혈액배포소A농도고우86～105 ng/ml,뇨액BKV-DNA병독검측양성,뇨액BKV-DNA병독재량수착혈액배포소A농도이개변,이자정선성관계.결론 당궤체처우면역억제상태시BKV병독-DNA적재량여사용면역억제제적제량유관,증가료이식후유발출혈성방광염적위험성.
Objective To explore the correlation between the polyomavirus DNA load and the dose of immunosuppressant in patients with allogene bone marrow transplantation (allo-BMT) for preventing the development of post-transplantational hemorrhagic cystitis. Methods Serial blood and urine samples from 122 cases of alIo-BMT recipients were obtained and DNA was extracted from urine samples. Polyomavirus DNA-specific probe was synthesized and Fluorescence quantitative polymerase chain reaction was used for detecting the polyomavirus DNA loads and Fluorescence polarization immunoassay (FPIA) was performed for determining the dose of immunosuppressant Cyclosporin A (CsA) in blood. Results The altered polyomavirus DNA load in urine was followed by concentration of CsA in blood. When the concentration of CsA in blood was higher than 86～105 ng/ml, the positive rate of polyomavirus DNA load was significantly increased and both presented the linable correlation. Conclusion In immunosuppression condition, polyomavirus DNA load correlated to the dose of immunosuppressant, which increased the risk of post-transplantational hemorrhagic cystitis.