中国兽医学报
中國獸醫學報
중국수의학보
CHINESE JOURNAL OF VETERINARY SCIENCE
2009年
1期
97-105
,共9页
周虚%于淼瑛%刘立文%易康乐%李纯锦%陈璐%孙艳玲
週虛%于淼瑛%劉立文%易康樂%李純錦%陳璐%孫豔玲
주허%우묘영%류립문%역강악%리순금%진로%손염령
LH%LH、FSH受体%mRNA表达%初情期前母猪
LH%LH、FSH受體%mRNA錶達%初情期前母豬
LH%LH、FSH수체%mRNA표체%초정기전모저
dietary energy%LHR and FSHR%mRNA expression,prepubertal gilts
对9头50日龄、体质量为30 kg的初情期前杜×长×大三元杂交母猪进行长期日粮能量差异饲养试验.饲养结束后屠宰,取卵巢液氮保存.半定量RT-PCR检测每头母猪卵巢LH和FSH受体mRNA的含量.结果,高能组卵巢LH和FSH受体mRNA的含量显著高于中能组和低能组(P<0.05);而低能组显著低于中能组和高能组(P<0.05).表明,能量水平高的日粮可以显著地促进初情期前卵巢上LH和FSH受体在mRNA水平的表达,而能量水平不足的日粮则不利于这种表达.本文首次报道了日粮能量水平对母猪卵巢LH和FSH受体mRNA表达的影响.
對9頭50日齡、體質量為30 kg的初情期前杜×長×大三元雜交母豬進行長期日糧能量差異飼養試驗.飼養結束後屠宰,取卵巢液氮保存.半定量RT-PCR檢測每頭母豬卵巢LH和FSH受體mRNA的含量.結果,高能組卵巢LH和FSH受體mRNA的含量顯著高于中能組和低能組(P<0.05);而低能組顯著低于中能組和高能組(P<0.05).錶明,能量水平高的日糧可以顯著地促進初情期前卵巢上LH和FSH受體在mRNA水平的錶達,而能量水平不足的日糧則不利于這種錶達.本文首次報道瞭日糧能量水平對母豬卵巢LH和FSH受體mRNA錶達的影響.
대9두50일령、체질량위30 kg적초정기전두×장×대삼원잡교모저진행장기일량능량차이사양시험.사양결속후도재,취란소액담보존.반정량RT-PCR검측매두모저란소LH화FSH수체mRNA적함량.결과,고능조란소LH화FSH수체mRNA적함량현저고우중능조화저능조(P<0.05);이저능조현저저우중능조화고능조(P<0.05).표명,능량수평고적일량가이현저지촉진초정기전란소상LH화FSH수체재mRNA수평적표체,이능량수평불족적일량칙불리우저충표체.본문수차보도료일량능량수평대모저란소LH화FSH수체mRNA표체적영향.
To explore the effects of long-term treatment of different dietary energy levels on ovarian expression of mRNAs for LHR and FSHR,the present study was performed in nine growth-matched littermate crossbred (Land-race×Large White X Duroc) prepubertal gilts. At approximately 30 kg of body weight and 50 day of age,gilts were housed with individual feeding stalls and placed on a normal level of feeding for 90 days (dl-90) with free ac-cess to water and food throughout the whole research. From d91 ,littermates were divided and randomly assigned to one of the following three treatment lines for 3 weeks till d112:Group H,Group M, and Group L, fed the high energy level diet (n = 3, digestible energy 14.87 MJ/kg), moderate energy level diet (n = 3, digestible energy 12.39 MJ/ kg), and low energy level diet (n = 3, digestible energy 9.98 MJ/kg), respectively. When gilts were slaughtered on d112 after 3 weeks energy treatment, both ovaries of every gilts were collected,snap frozen in liquid nitrogen and re-tained at -80℃ for use to determine and analysis the relative amount of ovarian LHR and FSHR mRNAs using semi-quantitative RT-PCR. Results showed that the effect of dietary energy treatment was notable: the ovarian ex-pression of mRNAs for LHR and FSHR was significantly higher (P<0.05) in gilts treated with high energy diet compared to gilts treated with moderate and low energy diets, while gilts treated with low energy diet had a signifi-cantly lower (P<0.05) ovarian LHR and FSHR expression compared with gilts treated with moderate and high en-ergy diets. These results revealed that ovarian expression of I.HR and FSHR in prepubertal gilts increased as the lev-el of dietary energy intake elevated,i, e. , high energy diet can markedly enhance ovarian expression of mRNAs for LHR and FSHR,whereas energy deficit markedly suppress the expression.