CAJ | 학술논문

目的研究1株多药耐药铜绿假单胞菌的β-内酰胺酶基因、菌膜蛋白OprD2基因、整合子和转座子介导的各种耐药基因、16S rRNA甲基化酶及氨基糖苷类修饰基因以及氯霉素与四环素相关耐药基因的的存在情况.方法以K-B法测量其对抗菌药物的耐药性;以PCR法检测其相关耐药基因;以PCR直接全自动荧光法进行阳性基因测序.结果该株铜绿假单胞菌检出TEM基因OXA-10基因,且伴有OprD2缺失;转座子遗传标记MerA阳性,Ⅰ类整合子遗传标记qacE△1-Sull阳性;耐氨基糖苷类基因aac(3)-Ⅱ、aac(6')-Ⅱ、rmtB阳性,耐氯霉素基因cmlA阳性.结论造成本株铜绿假单胞菌多药耐药的主要原因是该菌株存在多种抗生素相关耐药基因.
목적 연구1주다약내약동록가단포균적β-내선알매기인、균막단백OprD2기인、정합자화전좌자개도적각충내약기인、16S rRNA갑기화매급안기당감류수식기인이급록매소여사배소상관내약기인적적존재정황.방법 이K-B법측량기대항균약물적내약성;이PCR법검측기상관내약기인;이PCR직접전자동형광법진행양성기인측서.결과 해주동록가단포균검출TEM기인OXA-10기인,차반유OprD2결실;전좌자유전표기MerA양성,Ⅰ류정합자유전표기qacE△1-Sull양성;내안기당감류기인aac(3)-Ⅱ、aac(6')-Ⅱ、rmtB양성,내록매소기인cmlA양성.결론 조성본주동록가단포균다약내약적주요원인시해균주존재다충항생소상관내약기인.
Objective To explore the situation of β-lactamase gene, oprD2 gene of outer membrane protein, various drug resistance genes mediated by integron and transposon, 16S rRNA methylose gene and aminoglycoside modifying enzyme gene, as well as chloramphenicol- and tetracycline-related resistance genes in 1 strain of multidrug resistant Pseudomonas aeruginosa. Methods The antibiotics susceptibility was tested by K-B method; the drug resistance related genes were detected by polymerase chain reaction (PCR) ; the positive genes were ampilified and sequenced by PCR fluorescence spectrophotometry. Results The genes of TEM type OXA-10, qacE△1-Sull, aac(3)-Ⅱ, aac(6')-Ⅱ,rmtB and cmlA were found in the Pseudomonas aeruginosa strain, and OprD2 was negative. Conclusion The main reason for the Pseudomonas aeruginosa strain drug resistance is existence of multi-antiobiotic related drug resistance genes in the Pseudomonas aeruginosa strain.