河北北方学院学报：自然科学版
河北北方學院學報：自然科學版
하북북방학원학보：자연과학판
Journa of Hebei North University:Natural Science Edition
2011年
6期
59-61,69
,共4页
食管腺癌%癌前病变%DNA含量%倍体变化
食管腺癌%癌前病變%DNA含量%倍體變化
식관선암%암전병변%DNA함량%배체변화
esophageal adenocareinoma%precancerous lesions%DNA content%ploidy changes
目的探讨慢性炎症-癌前病变-食管腺癌不同阶段的食管黏膜细胞DNA含量及倍体变化，从细胞代谢水平揭示食管癌的发展过程．方法用流式细胞术对15例检查未见器质性病变者的正常组织，27例胃食管反流病患者（GERD）、25例Barrett食管患者和31例食管腺癌患者的病变组织细胞DNA含量进行了检测，并对四组的DNA倍体类型、DNA指数（DI）、G2／G1、增殖活性（SPF）和细胞增殖活性指数（PI）做了对比分析．结果食管腺癌22例DNA含量全部为非整倍体变化（DNA指数DI〉1．1），Barrett食管25例中仅6例为非整倍体DNA含量，胃食管反流病和健康者无1例为非整倍体DNA含量；食管腺癌组G2／G1、SPF、PI较正常组织和胃食管反流病组均有明显升高（P〈0．05），Barrett食管组介于胃食管反流病与食管腺癌之间，并且G2／G1、SPF和PI指标差异均有统计学意义（P〈0．05）．流式细胞术诊断阳性率差异显著．结论食管癌的发展是一个渐进的过程，各阶段之间密切相关．
目的探討慢性炎癥-癌前病變-食管腺癌不同階段的食管黏膜細胞DNA含量及倍體變化，從細胞代謝水平揭示食管癌的髮展過程．方法用流式細胞術對15例檢查未見器質性病變者的正常組織，27例胃食管反流病患者（GERD）、25例Barrett食管患者和31例食管腺癌患者的病變組織細胞DNA含量進行瞭檢測，併對四組的DNA倍體類型、DNA指數（DI）、G2／G1、增殖活性（SPF）和細胞增殖活性指數（PI）做瞭對比分析．結果食管腺癌22例DNA含量全部為非整倍體變化（DNA指數DI〉1．1），Barrett食管25例中僅6例為非整倍體DNA含量，胃食管反流病和健康者無1例為非整倍體DNA含量；食管腺癌組G2／G1、SPF、PI較正常組織和胃食管反流病組均有明顯升高（P〈0．05），Barrett食管組介于胃食管反流病與食管腺癌之間，併且G2／G1、SPF和PI指標差異均有統計學意義（P〈0．05）．流式細胞術診斷暘性率差異顯著．結論食管癌的髮展是一箇漸進的過程，各階段之間密切相關．
목적탐토만성염증-암전병변-식관선암불동계단적식관점막세포DNA함량급배체변화，종세포대사수평게시식관암적발전과정．방법용류식세포술대15례검사미견기질성병변자적정상조직，27례위식관반류병환자（GERD）、25례Barrett식관환자화31례식관선암환자적병변조직세포DNA함량진행료검측，병대사조적DNA배체류형、DNA지수（DI）、G2／G1、증식활성（SPF）화세포증식활성지수（PI）주료대비분석．결과식관선암22례DNA함량전부위비정배체변화（DNA지수DI〉1．1），Barrett식관25례중부6례위비정배체DNA함량，위식관반류병화건강자무1례위비정배체DNA함량；식관선암조G2／G1、SPF、PI교정상조직화위식관반류병조균유명현승고（P〈0．05），Barrett식관조개우위식관반류병여식관선암지간，병차G2／G1、SPF화PI지표차이균유통계학의의（P〈0．05）．류식세포술진단양성솔차이현저．결론식관암적발전시일개점진적과정，각계단지간밀절상관．
Objective To investigate the esophageal nuclear DNA content and ploidy changes of chronic inflammation-precancerous lesion-esophageal adenocarcinoma at different stages,and reveal that the development of esophageal cancer is a progressive process from view of cell metabolism. Methods For 15 patients without evidence of organic lesions in normal tissue,27 cases with gastroesophageal reflux（GERD）, 25 cases with Barrett and 31 cases with esophageal adenocarcinoma, flow cytometry was used to test cell DNA content, and for DNA ploidy, DNA index（DI）, G2/G1 , proliferative activity（SPF） and proliferation index（PI）of four groups,comparative analysis was done. Results For 22 cases with esophageal adenocarcinoma, all aneuploid changes happened on DNA content （DNA index DI〉1. 1）. But for Barrett esophagus, only six of 25 cases had aneuploid DNA content. In the end, for gastroesophageal reflux disease and healthy objects,no aneuploid DNA content happened. Compared with that of normal tissue and gastroesophageal reflux disease groups, index of G2/G1, SPF, PI for esophageal adenocarcinoma group were significantly higher（ P 〈0.05） ;while the index of Barrett esophagus group were between that of gastroesophageal reflux disease esophageal adenocarcinoma groups,G2/G1 ,SPF and PI indices were significant different, P 〈0. 05; flow cytometry diagnostic produced significant differences. Conclusion Esophageal cancer development is a progressive process,and there is a strong correlation between the various stages.
