CAJ | 학술논문

研究3种典型铁/硫代谢菌-Acidithiobacillus ferrooxidans,Leptospirillum ferriphilum及Acidithiobacillus thiooxidans混合浸出黄铜矿过程中铁/硫氧化活性、群落结构(PCR-RFLP)的变化,以及不同浓度的元素硫对其影响.结果发现,加入3.193 g/L元素硫能促进细菌的表观硫氧化活性,改变浸矿体系的群落结构,并进一步影响钝化层的形成、金属离子的溶出,其浸出率(71％)较未添加硫的(67％)有一定程度的提高.而过量的元素硫会抑制铜的浸出(浸出率44％).
연구3충전형철/류대사균-Acidithiobacillus ferrooxidans,Leptospirillum ferriphilum급Acidithiobacillus thiooxidans혼합침출황동광과정중철/류양화활성、군락결구(PCR-RFLP)적변화,이급불동농도적원소류대기영향.결과발현,가입3.193 g/L원소류능촉진세균적표관류양화활성,개변침광체계적군락결구,병진일보영향둔화층적형성、금속리자적용출,기침출솔(71％)교미첨가류적(67％)유일정정도적제고.이과량적원소류회억제동적침출(침출솔44％).
To estimate the relationships among bioleaching performance,additional elemental sulfur (S0),microbial population dynamics and its energy metabolism,bioleaching of chalcopyrite by three typical sulfur- and/or iron-oxidizing bacteria,Acidithiobacillus ferrooxidans,Leptospirillum ferriphilum and Acidithiobacillus thiooxidans with different levels of sulfur were studied in batch shake flask cultures incubated at 30 ℃.Copper dissolution capability (71％) was increased with the addition of 3.193 g/L S0,compared to that (67％) without S0.However,lower copper extraction was obtained in bioleaching with excessive sulfur.Microbial population dynamics during chalcopyrite bioleaching process was monitored by using PCR-restrietion fragment length polymorphism (PCR-RFLP).Additional S0 accelerated the growth of sulfur-oxidizing bacteria,inhibited the iron-oxidizing metabolism and led to the decrease of iron-oxidizing microorganisms,finally affected iron concentration,redox potential and bioleaching performance.It is suggested that mixed iron and sulfur-oxidizing microorganisms with further optimized additional S0 concentration could improve copper recovery from chalcopyrite.