CAJ | 학술논문

目的分析血管内皮生长因子(VEGF)及其受体KDR、Flt1在正常核型急性髓系白血病(NC-AML)中的表达和意义.方法用RT-PCR方法检测VEGF及其受体KDR、Flt1 mRNA表达,荧光定量PCR分析细胞中VEGF及其受体表达丰度.结果 45例NC-AML患者骨髓单核细胞(BMMNC)中,表达VEGF、KDR及Flt1 mRNA阳性的例数分别为42例(93.3%)、34例(75.6%)和37例(82.2%),而10名健康对照者经RT-PCR未检测到VEGF及其受体表达.筛选符合要求的标本进行荧光定量PCR检测,发现化疗前后细胞VEGF的表达存在显著性差异(P<0.05),化疗后获完全缓解的患者细胞VEGF表达水平仍较10名健康对照者升高(P<0.05),但其受体表达量与对照组比较未见显著性差异.结论 NC-AML白血病细胞不同程度表达VEGF及其受体,其中VEGF表达水平明显升高,通过对细胞VEGF表达水平的检测,对评估NC-AML白血病的进展、预测患者预后以及对微量残留白血病细胞的监测具有重要意义.
목적 분석혈관내피생장인자(VEGF)급기수체KDR、Flt1재정상핵형급성수계백혈병(NC-AML)중적표체화의의.방법 용RT-PCR방법검측VEGF급기수체KDR、Flt1 mRNA표체,형광정량PCR분석세포중VEGF급기수체표체봉도.결과 45례NC-AML환자골수단핵세포(BMMNC)중,표체VEGF、KDR급Flt1 mRNA양성적례수분별위42례(93.3%)、34례(75.6%)화37례(82.2%),이10명건강대조자경RT-PCR미검측도VEGF급기수체표체.사선부합요구적표본진행형광정량PCR검측,발현화료전후세포VEGF적표체존재현저성차이(P<0.05),화료후획완전완해적환자세포VEGF표체수평잉교10명건강대조자승고(P<0.05),단기수체표체량여대조조비교미견현저성차이.결론 NC-AML백혈병세포불동정도표체VEGF급기수체,기중VEGF표체수평명현승고,통과대세포VEGF표체수평적검측,대평고NC-AML백혈병적진전、예측환자예후이급대미량잔류백혈병세포적감측구유중요의의.
Objective To evaluate the expression and role of vascular endothelial growth factor (VEGF) and its receptors ( KDR and Flt1) in acute myeloid leukemia with normal cytogenetics (NC- AML). Methods The expression and abundance of VEGF and its receptors mRNA were assayed by RT- PCR and fluorescent quantitative RT-PCR (FQ-RT-PCR). Results Among 45 NC-AML patients, the positive rate of VEGF,KDR and FLt1 mRNA in the bone marrow mononuclear cells (BMMNC) were 93.3% ,75.6% and 82.2% ,respectively. However,the expression of VEGF,KDR and FLt1 mRNA in 10 health people was negative. Real time PCR confirmed the significant variation (P<0. 05) of the VEGF expression if the patient received a chemo therapy. In patients with complete remission(CR), the VEGF expression of VEGF was lower than pre-treatment but still remained relatively higher compared with 10 health people (P<0. 05). However, there was no significant difference for VEGF receptor expression, its receptors had no such significant difference. Conclusion VEGF and its receptors (KDR and Flt1) mRNAs were expressed distinctively in NC-AML ,while the VEGF shows higher levels. Thus ,the assay of VEGF expression levels is of great significance to evaluate the progression, the indication of prognosis and the monitor of micro residual disease.