中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2008年
3期
158-162
,共5页
肝炎,乙型,慢件%干扰素Ⅱ型%白细胞介素4%白细胞介素17
肝炎,乙型,慢件%榦擾素Ⅱ型%白細胞介素4%白細胞介素17
간염,을형,만건%간우소Ⅱ형%백세포개소4%백세포개소17
Hepatitis B,chronic%Interferon type Ⅱ%Interleukin-4%Interleukin-17
目的 了解慢性HBV感染中辅助性T淋巴细胞(Th)1、Th2、Thl7产生的主要细胞因子的表达,以反映这3种细胞的功能状态.方法 ELISA测定34例慢性乙型肝炎、31例肝硬化、26例原发性肝癌、22例慢性重型乙型肝炎患者及15例健康对照者m清中的1FN-γ、IL-4和IL-17水平,对数据进行t检验和Pearson相关分析.结果 IFN-γ/IL-4在慢性乙型肝炎患者(1.08±0.66)比健康对照者(2.60±0.60)降低(P＜0.01),在慢性重型乙型肝炎患者(4.81±0.87)比健康对照者升高(P＜0.01);IFN-γ/IL-17在慢性乙型肝炎(1.13±0.85,P＜0.01)、肝硬化(1.69±0.92,P=0.010)、肝癌(1.76±0.84,P=0.011)患者比健康对照者(2.66±0.70)降低,在慢性重型乙型肝炎患者(3.68±0.42)比健康对照者升高(P=0.004);IL-4/IL-17在肝硬化(0.72±0.38,P=0.026)、肝癌(0.63±0.19,P＜0.01)患者比健康对照者(1.04±0.23)降低.结论 慢性乙型肝炎患者IL-4的表达占优势;肝硬化、肝癌患者IL-17的表达占优势;慢性重型乙型肝炎患者IFN-γ的表达占优势.
目的 瞭解慢性HBV感染中輔助性T淋巴細胞(Th)1、Th2、Thl7產生的主要細胞因子的錶達,以反映這3種細胞的功能狀態.方法 ELISA測定34例慢性乙型肝炎、31例肝硬化、26例原髮性肝癌、22例慢性重型乙型肝炎患者及15例健康對照者m清中的1FN-γ、IL-4和IL-17水平,對數據進行t檢驗和Pearson相關分析.結果 IFN-γ/IL-4在慢性乙型肝炎患者(1.08±0.66)比健康對照者(2.60±0.60)降低(P＜0.01),在慢性重型乙型肝炎患者(4.81±0.87)比健康對照者升高(P＜0.01);IFN-γ/IL-17在慢性乙型肝炎(1.13±0.85,P＜0.01)、肝硬化(1.69±0.92,P=0.010)、肝癌(1.76±0.84,P=0.011)患者比健康對照者(2.66±0.70)降低,在慢性重型乙型肝炎患者(3.68±0.42)比健康對照者升高(P=0.004);IL-4/IL-17在肝硬化(0.72±0.38,P=0.026)、肝癌(0.63±0.19,P＜0.01)患者比健康對照者(1.04±0.23)降低.結論 慢性乙型肝炎患者IL-4的錶達佔優勢;肝硬化、肝癌患者IL-17的錶達佔優勢;慢性重型乙型肝炎患者IFN-γ的錶達佔優勢.
목적 료해만성HBV감염중보조성T림파세포(Th)1、Th2、Thl7산생적주요세포인자적표체,이반영저3충세포적공능상태.방법 ELISA측정34례만성을형간염、31례간경화、26례원발성간암、22례만성중형을형간염환자급15례건강대조자m청중적1FN-γ、IL-4화IL-17수평,대수거진행t검험화Pearson상관분석.결과 IFN-γ/IL-4재만성을형간염환자(1.08±0.66)비건강대조자(2.60±0.60)강저(P＜0.01),재만성중형을형간염환자(4.81±0.87)비건강대조자승고(P＜0.01);IFN-γ/IL-17재만성을형간염(1.13±0.85,P＜0.01)、간경화(1.69±0.92,P=0.010)、간암(1.76±0.84,P=0.011)환자비건강대조자(2.66±0.70)강저,재만성중형을형간염환자(3.68±0.42)비건강대조자승고(P=0.004);IL-4/IL-17재간경화(0.72±0.38,P=0.026)、간암(0.63±0.19,P＜0.01)환자비건강대조자(1.04±0.23)강저.결론 만성을형간염환자IL-4적표체점우세;간경화、간암환자IL-17적표체점우세;만성중형을형간염환자IFN-γ적표체점우세.
Objective To explore the expression of interferon(IFN)-γ,interleukin(IL)-4 and IL-17 in the patients with hepatitis B virus(HBV)infection,which can reflect the function of helDer T lymphocyte(Th)1,Th2 and Th17 cells.Methods There were 34 chronic hepatitis B(CHB)Datients,31 liver cirrhosis(LC)patients,26 primary hepatocellular carcinoma(PHC)patients,22 chronic severe hepatitis patients and 15 healthy controls involved in our research.The levels of IFN-γ,IL-4 and IL-17 in serum of the patients with chronic HBV infection and healthy controls were determined by enzyme linked immunosorbent assays(ELISA). And the dala were analysed via t-test and Pearson correlation analysis.Results IFN-γ/IL-4 ratio of the CHB group(1.08±0.66,P＜0.01)was lower than that of healthy controls(2.60±0.60),and the ratio in the chronic severe hepatitis B group(4.81±0.87,P＜0.01)was higher than that in healthy control group.IFN-γIL-17 ratio in the CHB(1.13±0.85,P＜0.01),the LC(1.69±0.92,P=0.010)and the PHC group(1.76±0.84,P=0.011)were all lower than that in healthy conlrols group(2.66±0.70),and the ratio in the chronic severe hepatitis B group(3.68±0.42)was higher than that in healthy controls group (P=0.004).IL-4/IL-17 ratio in the LC(0.72±0.38,P=0.026)and the PHC group(0.63±0.19,P＜0.01)were both lower than that in heahhy controls(1.04±0.23).Conclusiolls The dominant expression of IL-4,IL-7 and IFN-γ is respectively found in the CHB patients,LC and PHC patients,and chronic severe hepatilis B patients.
