中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2012年
11期
1121-1124
,共4页
周振虎%闫瑞红%邢怀广%张伟
週振虎%閆瑞紅%邢懷廣%張偉
주진호%염서홍%형부엄%장위
分化型甲状腺癌%血管内皮生长因子%内皮抑素
分化型甲狀腺癌%血管內皮生長因子%內皮抑素
분화형갑상선암%혈관내피생장인자%내피억소
Differentiated thyroid cancer%Vascular endothelial growth factor%Endostatin
目的 探讨血管内皮生长因子(VEGF)及内皮抑素在分化型甲状腺癌手术前后及复发时的表达.方法 分化型甲状腺癌术后肺转移患者7例(肺转移组),局部复发患者21例(局部复发组),选择甲状腺组织结构及功能正常者30例为对照组,分别采用酶联免疫吸附法检测血清VEGF及内皮抑素,化学发光法检测血清甲状腺球蛋白的浓度,应用SPSS 13.0统计学软件包分析,计量资料数据比较应用t检验,VEGF、内皮抑素与甲状腺球蛋白浓度的相关性采用Pearson等级相关分析.结果 肺转移组手术前VEGF、内皮抑素分别为(412.6±57.3)、(34.3±7.6) ng/L,术后3周分别为(210.3 ±30.4)、(25.2 ±6.2)ng/L,复发时分别为(619.5±126.4)、(38.6±8.7) ng/L；局部复发组手术前VEGF、内皮抑素分别为(399.4 ±56.3)、(35.2 ±6.8)ng/L,术后3周分别为(209.3 ±36.7)、(25.7 ±4.7) ng/L,复发时分别为(406.5±59.2)、(31.2±7.6) ng/L,组内比较术后3周显著低于手术前和复发时(P均＜0.05),组间比较复发时差异均有统计学意义(P均＜0.05).甲状腺癌术后复发患者血清甲状腺球蛋白与VEGF及内皮抑素表达成正相关(r值分别为0.752、0.349,P均＜0.01).结论 VEGF、内皮抑素在分化型甲状腺癌术前及术后复发时高表达,可作为评估分化型甲状腺癌生物学行为的一项重要指标.
目的 探討血管內皮生長因子(VEGF)及內皮抑素在分化型甲狀腺癌手術前後及複髮時的錶達.方法 分化型甲狀腺癌術後肺轉移患者7例(肺轉移組),跼部複髮患者21例(跼部複髮組),選擇甲狀腺組織結構及功能正常者30例為對照組,分彆採用酶聯免疫吸附法檢測血清VEGF及內皮抑素,化學髮光法檢測血清甲狀腺毬蛋白的濃度,應用SPSS 13.0統計學軟件包分析,計量資料數據比較應用t檢驗,VEGF、內皮抑素與甲狀腺毬蛋白濃度的相關性採用Pearson等級相關分析.結果 肺轉移組手術前VEGF、內皮抑素分彆為(412.6±57.3)、(34.3±7.6) ng/L,術後3週分彆為(210.3 ±30.4)、(25.2 ±6.2)ng/L,複髮時分彆為(619.5±126.4)、(38.6±8.7) ng/L；跼部複髮組手術前VEGF、內皮抑素分彆為(399.4 ±56.3)、(35.2 ±6.8)ng/L,術後3週分彆為(209.3 ±36.7)、(25.7 ±4.7) ng/L,複髮時分彆為(406.5±59.2)、(31.2±7.6) ng/L,組內比較術後3週顯著低于手術前和複髮時(P均＜0.05),組間比較複髮時差異均有統計學意義(P均＜0.05).甲狀腺癌術後複髮患者血清甲狀腺毬蛋白與VEGF及內皮抑素錶達成正相關(r值分彆為0.752、0.349,P均＜0.01).結論 VEGF、內皮抑素在分化型甲狀腺癌術前及術後複髮時高錶達,可作為評估分化型甲狀腺癌生物學行為的一項重要指標.
목적 탐토혈관내피생장인자(VEGF)급내피억소재분화형갑상선암수술전후급복발시적표체.방법 분화형갑상선암술후폐전이환자7례(폐전이조),국부복발환자21례(국부복발조),선택갑상선조직결구급공능정상자30례위대조조,분별채용매련면역흡부법검측혈청VEGF급내피억소,화학발광법검측혈청갑상선구단백적농도,응용SPSS 13.0통계학연건포분석,계량자료수거비교응용t검험,VEGF、내피억소여갑상선구단백농도적상관성채용Pearson등급상관분석.결과 폐전이조수술전VEGF、내피억소분별위(412.6±57.3)、(34.3±7.6) ng/L,술후3주분별위(210.3 ±30.4)、(25.2 ±6.2)ng/L,복발시분별위(619.5±126.4)、(38.6±8.7) ng/L；국부복발조수술전VEGF、내피억소분별위(399.4 ±56.3)、(35.2 ±6.8)ng/L,술후3주분별위(209.3 ±36.7)、(25.7 ±4.7) ng/L,복발시분별위(406.5±59.2)、(31.2±7.6) ng/L,조내비교술후3주현저저우수술전화복발시(P균＜0.05),조간비교복발시차이균유통계학의의(P균＜0.05).갑상선암술후복발환자혈청갑상선구단백여VEGF급내피억소표체성정상관(r치분별위0.752、0.349,P균＜0.01).결론 VEGF、내피억소재분화형갑상선암술전급술후복발시고표체,가작위평고분화형갑상선암생물학행위적일항중요지표.
Objective To evaluate the expression of vascular endothelial growth factor (VEGF)and endostatin (ES) in the serum of patients with differentiated thyroid cancer at the time of before and after operation and postoperative recurrence. Methods The serum samples were obtained from 7 patients with postoperative lung metastasis and 21 patients with local recurrence after operation.The serum samples from 30 normal subjects were obtained as control.The levels of serum VEGF and serum endostatin were analyzed by enzyme-linked immunosorbent assay (ELISA).The levels of serum thyroglobulin were analyzed by Chemiluminescence method.Statistical analysis was performed with t-test using SPSS 13.0 software.Pearson correlation analysis was performed for the correlation between serum VEGF and serum ES and thyroglobulin.Results The levels of serum VEGF and serum ES in patients with lung metastasis 3 weeks after operation were significantly lower than those before operation and at the time of recurrence [ VEGF:( 210.3 ± 30.4) ng/L vs (412.6 ±57.3) ng/L and(619.5 ± 126.4) ng/L,P ＜0.05 ;ES:(25.2 ±6.2)ng/L vs (34.3 ±7.6)ng/L and (38.6 ± 8.7) ng/L,P ＜ 0.05 ].And the levels of serum VEGF and serum ES in patients with local regional recurrence 3 weeks after operation were significantly lower than those before operation and at the time of recurrence [ VEGF:(209.3 ±36.7) ng/L vs (399.4 ±56.3) ng/L and (406.5 ±59.2) ng/L,P ＜0.05;ES:(25.7 ± 4.7 ) ng/L vs ( 35.2 ± 6.8 ) ng/L and ( 31.2 ± 7.6 ) ng/L,P ＜ 0.05 ].The expression of serum VEGF and serum ES in patients with pulmonary metastasis were significantly higher than those in patients with local regional postoperative recurrence ( P ＜ 0.05 ).There was a linear positive correlation between serum VEGF,serum ES and thyroglobulin levels in the patient with recurrent differentiated thyroid cancer( r =0.752,0.349,P ＜0.01 ).Conclusion The serum VEGF and serum ES level were significantly elevated in patients with differentiated thyroid cancer before operation and recurrence.The serum VEGF and serum ES were importantindicators to reveal the biological behaviors of differentiated thyroid cancer.
