CAJ | 학술논문

目的分析肝细胞生长因子(HGF)和c-Met与胃癌生物学行为和预后的关系。方法采用HGF、C-Met、Lyve-1和CD34对58例胃癌组织作免疫组织化学染色并计数，结合胃癌的临床病理资料作相关统计学分析。结果胃癌组织中HGF和c-Met表达明显上调，其微血管密度(26±10)和微淋巴管密度(24±9)均高于胃溃疡组织[(20±4)、(12±4)]，差异均有统计学意义(P＜0.01)；有淋巴转移的胃癌组织中HGF和c-Met的阳性表达率分别为86％和80％，高于无淋巴转移的胃癌组织(57％、36％)，差异均有统计学意义(P＜0.05)；有远处转移的胃癌组织中HGF和c-Met阳性表达率分别为100％和94％，高于无远处转移的胃癌组织(71％、60％)，差异均有统计学意义(P＜0.05)；HGF和c-Met表达与肿瘤是否有淋巴转移、远处转移和微血管密度、微淋巴管密度之间有明显的正相关关系(P＜0.01)；HGF、c-Met阳性和阴性组的生存率之间比较差异有统计学意义(P=0.09、P＜0.01)；肿瘤侵袭深度、有无淋巴转移、有无远处转移、胃癌的HGF、c-Met表达和微血管密度、微淋巴管密度均是影响胃癌预后的独立因素(P＜0.05)。结论 HGF和c-Met与胃癌的发生发展和预后密切相关，可以作为反映胃癌生物学行为和判断预后的有效指标。
목적 분석간세포생장인자(HGF)화c-Met여위암생물학행위화예후적관계。방법 채용HGF、C-Met、Lyve-1화CD34대58례위암조직작면역조직화학염색병계수，결합위암적림상병리자료작상관통계학분석。결과위암조직중HGF화c-Met표체명현상조，기미혈관밀도(26±10)화미림파관밀도(24±9)균고우위궤양조직[(20±4)、(12±4)]，차이균유통계학의의(P＜0.01)；유림파전이적위암조직중HGF화c-Met적양성표체솔분별위86％화80％，고우무림파전이적위암조직(57％、36％)，차이균유통계학의의(P＜0.05)；유원처전이적위암조직중HGF화c-Met양성표체솔분별위100％화94％，고우무원처전이적위암조직(71％、60％)，차이균유통계학의의(P＜0.05)；HGF화c-Met표체여종류시부유림파전이、원처전이화미혈관밀도、미림파관밀도지간유명현적정상관관계(P＜0.01)；HGF、c-Met양성화음성조적생존솔지간비교차이유통계학의의(P=0.09、P＜0.01)；종류침습심도、유무림파전이、유무원처전이、위암적HGF、c-Met표체화미혈관밀도、미림파관밀도균시영향위암예후적독립인소(P＜0.05)。결론 HGF화c-Met여위암적발생발전화예후밀절상관，가이작위반영위암생물학행위화판단예후적유효지표。
Objective To investigate the relationship between HGF/c-Met and biological behavior and prognosis of gastric carcinoma. Methods The expression of HGF.c-Met.microvessel density and microlymphatic densitv in 58 cases of gastric carcinoma and 27 cases of gastric ulcer were evaluated by immunohistochemistry，using monoclonal antibodies for HGF，c-Met，CD34 and Lyve-1 respectively.Results HGF and c-Met protein were overexpressed in gastric carcinoma，and were significantly higher than those in the mucosa of gastric ulcer(P＜0.01).The microvessel density and microlymphatic density of gastric carcinoma were significantlv different between those with and those without lymphatic metastasis and distant metastasis(all P＜0.05)：The positive expression of HGF and c-Met in those with lymph node metastasis(86％，80％)was significantly higher than those without lymph node metastasis(57％，36％)，P＜0.05.That with distant metastasis(100％.94％)was higher than that without(71％，60％)，P＜0.01.The 5 year survival rate in patients with positive HGF and c-Met expression was much lower than that with negative expression(P=0.019，P＜0.01)，Multivariate analysis indicated HGF and c-Met expression，depth of invasion.lymph node metastasis.distant metastasis，microvessel density and microlymphatic density were all independent prognostic factors of gastric carcinoma. Conclusions Expression of HGF and c-Met closely correlated with carcinogenesis，development and prognosis of gastric carcinoma.