CAJ | 학술논문

目的探讨盐酸小檗碱( berberine，BBR)对白念珠菌的体外抗真菌活性。方法采用美国临床实验室标准化委员会(现称美国临床实验室标准化研究所，CLSI)制订的M27 -A方案中的酵母菌微量稀释法，测定BBR、氟康唑、BBR联合氟康唑对30株临床分离白念珠菌的抗菌活性。结果盐酸小檗碱对白念珠菌MIC值的几何均数(G)为248.18 mg/L。与320、160、80 mg/L BBR联合后，氟康唑MIC值的几何均数从2.64 mg/L降为0.03、0.35、1.35 mg/L。两药对26.7％的白念菌株有协同抗菌作用，其中包括3株耐氟康唑菌，对56.7％的菌株表现为相加作用，对16.7％的菌株为无关作用，未观察到拮抗作用。结论 BBR在体外对白念珠菌具有抗真菌作用并能增加其对氟康唑的敏感性。
목적 탐토염산소벽감( berberine，BBR)대백념주균적체외항진균활성。방법 채용미국림상실험실표준화위원회(현칭미국림상실험실표준화연구소，CLSI)제정적M27 -A방안중적효모균미량희석법，측정BBR、불강서、BBR연합불강서대30주림상분리백념주균적항균활성。결과 염산소벽감대백념주균MIC치적궤하균수(G)위248.18 mg/L。여320、160、80 mg/L BBR연합후，불강서MIC치적궤하균수종2.64 mg/L강위0.03、0.35、1.35 mg/L。량약대26.7％적백념균주유협동항균작용，기중포괄3주내불강서균，대56.7％적균주표현위상가작용，대16.7％적균주위무관작용，미관찰도길항작용。결론 BBR재체외대백념주균구유항진균작용병능증가기대불강서적민감성。
Objective To test the antifungal activity of berberine (BBR) against Candida albicans in vitro. Methods According to the National Committee for Clinical Laboratory Standards (NCCLS) M27-A protocol, broth microdilution test was performed to assess the susceptibility of 30 clinical isolates of Candida albicans to BBR and fluconazole alone or in combination. Results The geometric mean minimum inhibitory concentration (MIC) was 248.18 mg/L and 2.64 mg/L for BBR and fluconazole alone, respectively. When combined with BBR of 320, 160 and 80 mg/L, the geometric mean MIC decreased to 0.03, 0.35 and 1.35 mg/L respectively for fluconazole. Fluconazole and BBR showed synergistic effects in 26.7％ of these isolates including 3 fluconazoleresistant strains, additive effects in 56.7％, indifferent effects in 16.7％, antagonistic effects in none. Conclu sion In vitro, BBR has antifungal activity against Candida albicans and can enhance the activity of fluconazole against Candida albicans.