国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2011年
10期
731-735,封3
,共6页
肖平%陈建荣%蔡映云%陈金亮%杨其昌%陶国华%陆晨希%陶一江
肖平%陳建榮%蔡映雲%陳金亮%楊其昌%陶國華%陸晨希%陶一江
초평%진건영%채영운%진금량%양기창%도국화%륙신희%도일강
p53蛋白%呼出气冷凝液%非小细胞肺癌
p53蛋白%呼齣氣冷凝液%非小細胞肺癌
p53단백%호출기냉응액%비소세포폐암
p53 protein%Exhaled breath condensate%Non-small cell lung cancer
目的 研究非小细胞肺癌(NSCLC)患者呼出气冷凝液(EBC)中p53蛋白检测的临床意义.方法 收集98例NSCLC患者的EBC和血浆,应用双抗体夹心ABC-ELISA法检测EBC和血浆中p53蛋白表达水平,并与98名健康对照者测定值比较.用免疫组化法检测98例NSCLC患者手术切除标本癌组织中p53蛋白表达.比较肺癌组中不同分型、分期、病理类型、肿瘤大小以及有无淋巴结转移、吸烟史者EBC和血浆中p53蛋白水平和癌组织p53蛋白阳性表达率.应用 ROC曲线分析肺癌组血浆及EBC的p53蛋白诊断肺癌的特异性及敏感性.结果 ①肺癌组EBC中p53蛋白测定值高于健康对照组[(233.99±7.91)ng/L vs(130.26±4.73)ng/L,P＜0.01];肺癌组血浆p53蛋白测定值高于健康对照组[(292.58±8.79)ng/L vs(141.66±3.33)ng/L,P＜0.01].②肺癌组中央型患者EBC中p53蛋白测定值高于周围型患者[(248.22±8.58)ng/L vs(215.78±6.61)ng/L,P＜0.05].③免疫组化阳性组EBC中p53蛋白测定值高于阴性组「(249.77±8.07)ng/L vs(216.86±7.44)ng/L,P＜0.05].④肺癌组中吸烟者血浆p53蛋白测定值高于无吸烟者[(310.18±9.04)ng/L vs(254.55±6.91)ng/L,P＜0.01].⑤癌组织p53蛋白阳性表达率为47.96%(47/98).⑥血浆p53蛋白对于肺癌的诊断的敏感性为95.90%.特异性为90.04%;EBC p53蛋白埘于肺癌的诊断的敏感性为92.90%,特异性为79.59%;肺癌患者p53蛋白诊断最佳参考临界值血浆为175.68 ng/L,EBC为166.26 ng/L.结论 NSCLC患者中EBC p53蛋白的检测有助于肺癌的诊断.
目的 研究非小細胞肺癌(NSCLC)患者呼齣氣冷凝液(EBC)中p53蛋白檢測的臨床意義.方法 收集98例NSCLC患者的EBC和血漿,應用雙抗體夾心ABC-ELISA法檢測EBC和血漿中p53蛋白錶達水平,併與98名健康對照者測定值比較.用免疫組化法檢測98例NSCLC患者手術切除標本癌組織中p53蛋白錶達.比較肺癌組中不同分型、分期、病理類型、腫瘤大小以及有無淋巴結轉移、吸煙史者EBC和血漿中p53蛋白水平和癌組織p53蛋白暘性錶達率.應用 ROC麯線分析肺癌組血漿及EBC的p53蛋白診斷肺癌的特異性及敏感性.結果 ①肺癌組EBC中p53蛋白測定值高于健康對照組[(233.99±7.91)ng/L vs(130.26±4.73)ng/L,P＜0.01];肺癌組血漿p53蛋白測定值高于健康對照組[(292.58±8.79)ng/L vs(141.66±3.33)ng/L,P＜0.01].②肺癌組中央型患者EBC中p53蛋白測定值高于週圍型患者[(248.22±8.58)ng/L vs(215.78±6.61)ng/L,P＜0.05].③免疫組化暘性組EBC中p53蛋白測定值高于陰性組「(249.77±8.07)ng/L vs(216.86±7.44)ng/L,P＜0.05].④肺癌組中吸煙者血漿p53蛋白測定值高于無吸煙者[(310.18±9.04)ng/L vs(254.55±6.91)ng/L,P＜0.01].⑤癌組織p53蛋白暘性錶達率為47.96%(47/98).⑥血漿p53蛋白對于肺癌的診斷的敏感性為95.90%.特異性為90.04%;EBC p53蛋白塒于肺癌的診斷的敏感性為92.90%,特異性為79.59%;肺癌患者p53蛋白診斷最佳參攷臨界值血漿為175.68 ng/L,EBC為166.26 ng/L.結論 NSCLC患者中EBC p53蛋白的檢測有助于肺癌的診斷.
목적 연구비소세포폐암(NSCLC)환자호출기냉응액(EBC)중p53단백검측적림상의의.방법 수집98례NSCLC환자적EBC화혈장,응용쌍항체협심ABC-ELISA법검측EBC화혈장중p53단백표체수평,병여98명건강대조자측정치비교.용면역조화법검측98례NSCLC환자수술절제표본암조직중p53단백표체.비교폐암조중불동분형、분기、병리류형、종류대소이급유무림파결전이、흡연사자EBC화혈장중p53단백수평화암조직p53단백양성표체솔.응용 ROC곡선분석폐암조혈장급EBC적p53단백진단폐암적특이성급민감성.결과 ①폐암조EBC중p53단백측정치고우건강대조조[(233.99±7.91)ng/L vs(130.26±4.73)ng/L,P＜0.01];폐암조혈장p53단백측정치고우건강대조조[(292.58±8.79)ng/L vs(141.66±3.33)ng/L,P＜0.01].②폐암조중앙형환자EBC중p53단백측정치고우주위형환자[(248.22±8.58)ng/L vs(215.78±6.61)ng/L,P＜0.05].③면역조화양성조EBC중p53단백측정치고우음성조「(249.77±8.07)ng/L vs(216.86±7.44)ng/L,P＜0.05].④폐암조중흡연자혈장p53단백측정치고우무흡연자[(310.18±9.04)ng/L vs(254.55±6.91)ng/L,P＜0.01].⑤암조직p53단백양성표체솔위47.96%(47/98).⑥혈장p53단백대우폐암적진단적민감성위95.90%.특이성위90.04%;EBC p53단백시우폐암적진단적민감성위92.90%,특이성위79.59%;폐암환자p53단백진단최가삼고림계치혈장위175.68 ng/L,EBC위166.26 ng/L.결론 NSCLC환자중EBC p53단백적검측유조우폐암적진단.
Objective To study the clinical significance of the detection of p53 protein in exhaled breath condensate (EBC) of patients with non-small cell lung cancer (NSCLC). Methods EBC and plasma of 98 patients with NSCLC were collected,p53 protein expression in EBC and plasma was detected by enzyme-linked immunosorbent assay,and the data were compared with those of 98 healthy controls. p53 protein expression in cancer tissue of 98 patients with NSCLC was detected by immunohistochemistry. p53 protein expression in EBC and plasma and positive expression rate of p53 protein in cancer tissue were compared among patients with different lung cancer type,stage,histologic type,tumor size,and lymph node metastasis,smoking history. The specificity and sensitivity of diagnosis of p53 protein in patients with NSCLC were analyzed by ROC curve. Results ① The level of p53 protein in EBC of patients with NSCLC was significantly higher than that in healthy control group [(233.99±7.91) ng/L vs ( 130. 26 ± 4. 73) ng/L,P ＜0. 01]. The level of p53 protein in serum of patients with NSCI.C was significantly higher than that in healthy control group [(292. 58 ± 8. 79) ng/L vs (141. 66±3. 33) ng/L,P ＜0. 01]. ② The level of p53 protein in EBC of patients with central lung cancer was higher than that in patients with peripheral lung cancer [(248. 22 ± 8. 58) ng/L vs (215. 78 ± 6.61) ng/L,P＜0. 01]. ③The level of p53 protein in EBC of patients with positive immunostaining group was higher than that in negative group [(249.77 ± 8.07) ng/L vs (216.86 ± 7.44) ng/L,P ＜ 0. 05]. ④The level of p53 protein in serum of smokers was significantly higher than that in non-smokers [(310.18 ± 9.04) ng/L vs (254. 55 ± 6. 91) ng/L,P ＜0. 01]. ⑤The positive expression rate of p53 protein in cancer tissue was 47. 96% (47/98). ⑥The sensitivity and specificity of diagnosis of p53 protein were 95. 90% and 90. 04% in plasma,and those were 92. 90% and 79. 59% in EBC. The cut off values of p53 protein were respectively 175. 68 ng/L and 166. 26 ng/L in EBC and serum. Conclusions The detection of p53 protein in EBC of patients with NSCLC is helpful for the diagnosis of lung cancer.
