中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2012年
3期
279-282
,共4页
赵丽丽%陈洁%闫丽佳%裴俊瑞%高琳%李兴洲%刘明法%李少臣%周令望%刘增超%王铜
趙麗麗%陳潔%閆麗佳%裴俊瑞%高琳%李興洲%劉明法%李少臣%週令望%劉增超%王銅
조려려%진길%염려가%배준서%고림%리흥주%류명법%리소신%주령망%류증초%왕동
硒%蛋白质类%维生素E%硒蛋白质类%心脏损伤
硒%蛋白質類%維生素E%硒蛋白質類%心髒損傷
서%단백질류%유생소E%서단백질류%심장손상
Selenium%Proteins%Vitamin E%Selenoproteins%Heart injuries
目的 观察硒、蛋白质和维生素E(VE)对大鼠心肌硒蛋白mRNA表达的影响,探讨其与心肌损伤的关系.方法 将40只雄性Wistar大鼠分为4组:低硒低蛋白低VE组,低硒低蛋白常VE组,常硒常蛋白低VE组,常硒常蛋白常VE组,每组10只.6个月时,采用二硫双硝基苯甲酸(DTNB)法检测全血谷胱甘肽过氧化物酶(GSH-Px)活力；用实时荧光定量(Real -time,RT)PCR法检测心肌细胞内谷胱甘肽过氧化物酶1( Gpx1)、磷脂氢谷胱甘肽过氧化物酶4(Gpx4)、硫氧还蛋白还原酶(TrxR)、硒蛋白P(Se-P)和硒蛋白W(Se-W)mRNA表达；光镜下观察心肌组织病理学变化.结果 6个月时,低硒低蛋白低VE组、低硒低蛋白常VE组、常硒常蛋白低VE组、常硒常蛋白常VE组全血GSH-Px活力分别为(44.6±3.1)、(45.5±1.6)、(86.6±2.2)、(85.6±1.2)U/L,组间比较差异有统计学意义(F=100.7,P＜ 0.01).其中常硒常蛋白低VE组、常硒常蛋白常VE组的全血GSH-Px活力高于低硒低蛋白低VE组、低硒低蛋白常VE组(P均＜0.05).上述4组大鼠心肌组织Gpx1(0.099±0.312、0.054±0.007、0.386±0.067、0.340±0.085)、Gpx4( 1.005±0.089、0.810±0.229、0.895±0.084、0.922±0.399)、Se-W(0.188±0.080、0.119±0.069、0.574±0.167、0.570±0.383 )mRNA表达组间比较,差异有统计学意义(F值分别为112.1、3.76、22.8,P均＜0.05).其中常硒常蛋白低VE组、常硒常蛋白常VE组Gpx1、Se-W mRNA表达高于低硒低蛋白低VE组、低硒低蛋白常VE组(P均＜0.05),低硒低蛋白低VE组Gpx4 mRNA表达高于常硒常蛋白低VE组(P＜0.05).而上述4组大鼠心肌组织TrxR、Se-P mRNA表达水平(0.130±0.037、0.127±0.038、0.134±0.021、0.120±0.014,0.446±0.155、0.413±0.152、0.385±0.041、0.408±0.208)组间比较,差异无统计学意义(F值分别为0.91、1.75,P均＞0.05).光镜下上述4组大鼠心肌组织病理学改变主要表现为灶状凝固性坏死,坏死检出率分别为80％(8/10)、44％(4/9)、20％(2/10)、10％(1/10),组间比较差异有统计学意义(Fisher精确检验,P=0.0067).结论 长期低硒、低蛋白质和低VE摄入使机体抗氧化能力降低,导致心肌损伤的发生；Gpx1、Se-W mRNA表达与硒水平密切相关,Gpx4、TrxR、Se-P mRNA表达则未见明显相关性.
目的 觀察硒、蛋白質和維生素E(VE)對大鼠心肌硒蛋白mRNA錶達的影響,探討其與心肌損傷的關繫.方法 將40隻雄性Wistar大鼠分為4組:低硒低蛋白低VE組,低硒低蛋白常VE組,常硒常蛋白低VE組,常硒常蛋白常VE組,每組10隻.6箇月時,採用二硫雙硝基苯甲痠(DTNB)法檢測全血穀胱甘肽過氧化物酶(GSH-Px)活力；用實時熒光定量(Real -time,RT)PCR法檢測心肌細胞內穀胱甘肽過氧化物酶1( Gpx1)、燐脂氫穀胱甘肽過氧化物酶4(Gpx4)、硫氧還蛋白還原酶(TrxR)、硒蛋白P(Se-P)和硒蛋白W(Se-W)mRNA錶達；光鏡下觀察心肌組織病理學變化.結果 6箇月時,低硒低蛋白低VE組、低硒低蛋白常VE組、常硒常蛋白低VE組、常硒常蛋白常VE組全血GSH-Px活力分彆為(44.6±3.1)、(45.5±1.6)、(86.6±2.2)、(85.6±1.2)U/L,組間比較差異有統計學意義(F=100.7,P＜ 0.01).其中常硒常蛋白低VE組、常硒常蛋白常VE組的全血GSH-Px活力高于低硒低蛋白低VE組、低硒低蛋白常VE組(P均＜0.05).上述4組大鼠心肌組織Gpx1(0.099±0.312、0.054±0.007、0.386±0.067、0.340±0.085)、Gpx4( 1.005±0.089、0.810±0.229、0.895±0.084、0.922±0.399)、Se-W(0.188±0.080、0.119±0.069、0.574±0.167、0.570±0.383 )mRNA錶達組間比較,差異有統計學意義(F值分彆為112.1、3.76、22.8,P均＜0.05).其中常硒常蛋白低VE組、常硒常蛋白常VE組Gpx1、Se-W mRNA錶達高于低硒低蛋白低VE組、低硒低蛋白常VE組(P均＜0.05),低硒低蛋白低VE組Gpx4 mRNA錶達高于常硒常蛋白低VE組(P＜0.05).而上述4組大鼠心肌組織TrxR、Se-P mRNA錶達水平(0.130±0.037、0.127±0.038、0.134±0.021、0.120±0.014,0.446±0.155、0.413±0.152、0.385±0.041、0.408±0.208)組間比較,差異無統計學意義(F值分彆為0.91、1.75,P均＞0.05).光鏡下上述4組大鼠心肌組織病理學改變主要錶現為竈狀凝固性壞死,壞死檢齣率分彆為80％(8/10)、44％(4/9)、20％(2/10)、10％(1/10),組間比較差異有統計學意義(Fisher精確檢驗,P=0.0067).結論 長期低硒、低蛋白質和低VE攝入使機體抗氧化能力降低,導緻心肌損傷的髮生；Gpx1、Se-W mRNA錶達與硒水平密切相關,Gpx4、TrxR、Se-P mRNA錶達則未見明顯相關性.
목적 관찰서、단백질화유생소E(VE)대대서심기서단백mRNA표체적영향,탐토기여심기손상적관계.방법 장40지웅성Wistar대서분위4조:저서저단백저VE조,저서저단백상VE조,상서상단백저VE조,상서상단백상VE조,매조10지.6개월시,채용이류쌍초기분갑산(DTNB)법검측전혈곡광감태과양화물매(GSH-Px)활력；용실시형광정량(Real -time,RT)PCR법검측심기세포내곡광감태과양화물매1( Gpx1)、린지경곡광감태과양화물매4(Gpx4)、류양환단백환원매(TrxR)、서단백P(Se-P)화서단백W(Se-W)mRNA표체；광경하관찰심기조직병이학변화.결과 6개월시,저서저단백저VE조、저서저단백상VE조、상서상단백저VE조、상서상단백상VE조전혈GSH-Px활력분별위(44.6±3.1)、(45.5±1.6)、(86.6±2.2)、(85.6±1.2)U/L,조간비교차이유통계학의의(F=100.7,P＜ 0.01).기중상서상단백저VE조、상서상단백상VE조적전혈GSH-Px활력고우저서저단백저VE조、저서저단백상VE조(P균＜0.05).상술4조대서심기조직Gpx1(0.099±0.312、0.054±0.007、0.386±0.067、0.340±0.085)、Gpx4( 1.005±0.089、0.810±0.229、0.895±0.084、0.922±0.399)、Se-W(0.188±0.080、0.119±0.069、0.574±0.167、0.570±0.383 )mRNA표체조간비교,차이유통계학의의(F치분별위112.1、3.76、22.8,P균＜0.05).기중상서상단백저VE조、상서상단백상VE조Gpx1、Se-W mRNA표체고우저서저단백저VE조、저서저단백상VE조(P균＜0.05),저서저단백저VE조Gpx4 mRNA표체고우상서상단백저VE조(P＜0.05).이상술4조대서심기조직TrxR、Se-P mRNA표체수평(0.130±0.037、0.127±0.038、0.134±0.021、0.120±0.014,0.446±0.155、0.413±0.152、0.385±0.041、0.408±0.208)조간비교,차이무통계학의의(F치분별위0.91、1.75,P균＞0.05).광경하상술4조대서심기조직병이학개변주요표현위조상응고성배사,배사검출솔분별위80％(8/10)、44％(4/9)、20％(2/10)、10％(1/10),조간비교차이유통계학의의(Fisher정학검험,P=0.0067).결론 장기저서、저단백질화저VE섭입사궤체항양화능력강저,도치심기손상적발생；Gpx1、Se-W mRNA표체여서수평밀절상관,Gpx4、TrxR、Se-P mRNA표체칙미견명현상관성.
Objective To explore the effect of selenium,protein and vitamin E deficiency on mRNA expression of rat cardiac selenoprotein,and their relation with myocardial injury.Methods Forty male Wistar rats were randomly divided into 4 groups:low selenium low protein low vitamin E group(group A),low selenium low protein adequate vitamin E group(group B),adequate selenium adequate protein low vitamin E group(group C),and adequate selenium adequate protein adequate vitamin E group (group D),10 rats in each group.The activity of whole blood glùtathione peroxidase(GSH-Px ) was measured using dithiobis nitrobenzoic acid (DTNB) at the end of sixth month experiment.The levels of mRNA expression of glutathione peroxidase 1(Gpx1),phospholipid hydroperoxide glutathione peroxidase 4(Gpx4),thioredoxin reductase(TrxR),selenoprotein P(Se-P) and selenoprotein W(Se-W) were determined by real-time fluorescence quantitative PCR at the end of sixth month.Histopathological changes of myocardial injury were observed with light microscope.Results The activity of GSH-Px was (44.6 ± 3.1 ),(45.5 ± 1.6),(86.6 ± 2.2),(85.6 ± 1.2)U/L,respectively,in the above four groups at the end of sixth month,and the difference was statistically significant(F =100.7,P ＜ 0.01 ) ; the activity of GSH-Px of groups C and D was higher than that of groups A,B(all P ＜ 0.05).mRNA expression of myocardial tissue of the four groups was as follows,Gpx1(0.099 ± 0.312,0.054 ± 0.007,0.386 ± 0.067,0.340 ± 0.085),Gpx4(1.005 ± 0.089,0.810 ± 0.229,0.895 ± 0.084,0.922 ± 0.399),and Se-W(0.188 ± 0.080,0.119 ± 0.069,0.574 ± 0.167,0.570 ± 0.383),and the difference was statistically significant(F =112.1,3.76,22.8,all P ＜ 0.05) ; the mRNA levels of Gpx1,Se-W of groups C,D were significantly higher than that of groups A,B(all P ＜ 0.05).The mRNA expression of Gpx4 of group A was higher than that of group C(P ＜ 0.05).The mRNA expression of TrxR(0.130 ± 0.037,0.127 ± 0.038,0.134 ± 0.021,0.120 ± 0.014) and Se-P(0.446 ± 0.155,0.413 ± 0.152,0.385 ± 0.041,0.408 ±0.208 ) was not statistically different among the four groups (F =0.91,1.75,all P ＞ 0.05).Pathological changes of myocardial tissue were mainly as foci of coagulative necrosis.The necrosis detection rate of the four groups was 8/10,4/9,2/10,and 1/10,respectively,and the difference was significant statistically(Fisher exact test,P =0.0067).Conclusions Long-term selenium,protein and vitamin E deficiency will reduce body antioxidant capacity and lead to myocardial injury.The mRNA levels of Gpx1 and Se-W and selenium level are closely related.The mRNA levels of Gpx4,TrxR and Se-P remain relatively stable.
