CAJ | 학술논문

目的探讨皮肤鳞状细胞癌和食道鳞状细胞癌患者癌组织中人类疱疹病毒8型(HHV-8)K15等位基因型与肿瘤的发病关系。方法序列特异性引物-巢式PCR技术检测40例皮肤鳞状细胞癌、40例食道鳞状细胞癌石蜡包埋组织中HHV-8 K15基因，并确定HHV-8 K15等位基因型，运用x2检验对结果进行统计学分析。结果 9例皮肤鳞状细胞癌(22.5％)石蜡包埋组织中检测到HHV-8 K15P型，8例食道鳞状细胞癌(20％)石蜡包埋组织中检测到HHV- 8K15P型，两种肿瘤均未检测出K15M基因型。HHV-8在皮肤鳞状细胞癌与食道鳞状细胞癌感染率组间比较差异无统计学意义(P＞0.05)。结论鳞状细胞癌中感染HHV-8K15P基因为K15P等位基因型，其发生发展可能与HHV-8的感染有一定的关系。
목적 탐토피부린상세포암화식도린상세포암환자암조직중인류포진병독8형(HHV-8)K15등위기인형여종류적발병관계。방법 서렬특이성인물-소식PCR기술검측40례피부린상세포암、40례식도린상세포암석사포매조직중HHV-8 K15기인，병학정HHV-8 K15등위기인형，운용x2검험대결과진행통계학분석。결과 9례피부린상세포암(22.5％)석사포매조직중검측도HHV-8 K15P형，8례식도린상세포암(20％)석사포매조직중검측도HHV- 8K15P형，량충종류균미검측출K15M기인형。HHV-8재피부린상세포암여식도린상세포암감염솔조간비교차이무통계학의의(P＞0.05)。결론 린상세포암중감염HHV-8K15P기인위K15P등위기인형，기발생발전가능여HHV-8적감염유일정적관계。
Objective To study the relationship of HHV-8 K15 allelotypes in cutaneous and esophageal squamous cell carcinoma (SCC) tissue with tumorigenesis. Methods Sequence specific primernested PCR was performed to detect HHV-8 K15 gene and to determine its allelotype in paraffin-embedded tissue specimens from 40 patients with cutaneous SCC and 40 patients with esophageal SCC. Chi-square test was used for statistical analysis. Results HHV-8 K15 P allele was detected in 9 (22.5％) of the cutaneous SCC specimens and 8 (20％) of the esophageal SCC specimens. There was no significant difference in the detection rate of HHV-8 between cutaneous SCC and esophageal SCC specimens (P ＞ 0.05). HHV-8 K15 M allele was undetected in this study. Conclusions SCC tissues appear to harbor only HHV-8 K15 P allele, and HHV-8 may play a part in the initiation and progression of SCC.