中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2009年
3期
320-323
,共4页
陈丹燕%邓华聪%南静%冯正平%刘强%秦登优%周恒宇
陳丹燕%鄧華聰%南靜%馮正平%劉彊%秦登優%週恆宇
진단연%산화총%남정%풍정평%류강%진등우%주항우
Fox01%胰岛β细胞%半胱大冬蛋门酶3%细胞凋亡%糖尿病,实验性
Fox01%胰島β細胞%半胱大鼕蛋門酶3%細胞凋亡%糖尿病,實驗性
Fox01%이도β세포%반광대동단문매3%세포조망%당뇨병,실험성
Fox01%Pancreatic islet β-cell%Caspase-3%Apoptosis%Diabetes mellitus,experimental
以高糖高脂饲料及链脲佐菌素诱导SD大鼠建立2型糖尿病模型,结果发现叉头转录因子(Fox01)[细胞核内(15.00+1.15 vs 6.45±0.62)%,P<0.05]、半胱天冬蛋白酶3(caspase-3)[(23.73±1.48vs 6.30±2.20)%,P<0.01]在糖尿病大鼠胰岛β细胞的表达和β细胞凋亡率[(22.29±1.84 vs 6.25±2.42)%,P<0.01]均高于正常大鼠;并且Fox01(核内)与caspase-3高表达的胰岛细胞正是发生凋亡的胰岛细胞.因此,Fox01可能参与2型糖尿病胰岛β细胞凋亡的调控.
以高糖高脂飼料及鏈脲佐菌素誘導SD大鼠建立2型糖尿病模型,結果髮現扠頭轉錄因子(Fox01)[細胞覈內(15.00+1.15 vs 6.45±0.62)%,P<0.05]、半胱天鼕蛋白酶3(caspase-3)[(23.73±1.48vs 6.30±2.20)%,P<0.01]在糖尿病大鼠胰島β細胞的錶達和β細胞凋亡率[(22.29±1.84 vs 6.25±2.42)%,P<0.01]均高于正常大鼠;併且Fox01(覈內)與caspase-3高錶達的胰島細胞正是髮生凋亡的胰島細胞.因此,Fox01可能參與2型糖尿病胰島β細胞凋亡的調控.
이고당고지사료급련뇨좌균소유도SD대서건립2형당뇨병모형,결과발현차두전록인자(Fox01)[세포핵내(15.00+1.15 vs 6.45±0.62)%,P<0.05]、반광천동단백매3(caspase-3)[(23.73±1.48vs 6.30±2.20)%,P<0.01]재당뇨병대서이도β세포적표체화β세포조망솔[(22.29±1.84 vs 6.25±2.42)%,P<0.01]균고우정상대서;병차Fox01(핵내)여caspase-3고표체적이도세포정시발생조망적이도세포.인차,Fox01가능삼여2형당뇨병이도β세포조망적조공.
SD rats were injected with streptozotocin and fed with high fat diet,used as type 2 diabetic model. Transcription factor Fox01 [in nucleus (15.00±1. 15 vs 6.45±0. 62) %, P<0.05], Caspase-3 [(23.73 ±1.48 vs 6.30±2.20)% ,P<0.01] expressions in pancreatic istet β cells and the positive rate of islet β cells apoptosis[(22.29±1.84 vs 6.25±2.42) %, P<0.01] in diabetic rats were higher than those of control rats. The islet cells which highly expresed Fox01 (in nucleus)and caspase-3 were just the apoptotic islet cells. Therefore,Fox01 may be involved in regulating apoptosis of islet β cells in type 2 diabetes.
