CAJ | 학술논문

目的探讨直肠癌组织中碳酸酐酶Ⅸ(CA-Ⅸ)和血管内皮生长因子(VEGF)的表达及与临床病理的关系.方法应用蛋白免疫印迹方法 (Western blot)检测104例直肠癌、30例正常直肠组织、30例直肠腺瘤中CA-Ⅸ和VEGF的表达.结果直肠癌组织中CA-Ⅸ和VEGF阳性表达率分别为72.1%和65.4%,高于正常直肠组织和腺瘤组织(P<0.05).CA-Ⅸ的表达与Dukes'分期及淋巴结转移相关,Dukes'分期A至C期CA-Ⅸ阳性率下降(P<0.05),淋巴结转移组CA-Ⅸ阳性率低于无淋巴结转移组(P<0.05).直肠癌组织和系膜组织VEGF的表达与淋巴结转移关系密切,N2组VEGF阳性表达率分别为81.3%和75.0%,高于NO组的53.7%和25.9%(P<0.05).CA-Ⅸ和癌组织和系膜组织的VEGF的表达无明显相关(r=0.092和r=-0.292).结论 CA-Ⅸ和VEGF表达与直肠癌发生、发展和浸润转移有关,但两者作用机制不同.
목적 탐토직장암조직중탄산항매Ⅸ(CA-Ⅸ)화혈관내피생장인자(VEGF)적표체급여림상병리적관계.방법 응용단백면역인적방법 (Western blot)검측104례직장암、30례정상직장조직、30례직장선류중CA-Ⅸ화VEGF적표체.결과 직장암조직중CA-Ⅸ화VEGF양성표체솔분별위72.1%화65.4%,고우정상직장조직화선류조직(P<0.05).CA-Ⅸ적표체여Dukes'분기급림파결전이상관,Dukes'분기A지C기CA-Ⅸ양성솔하강(P<0.05),림파결전이조CA-Ⅸ양성솔저우무림파결전이조(P<0.05).직장암조직화계막조직VEGF적표체여림파결전이관계밀절,N2조VEGF양성표체솔분별위81.3%화75.0%,고우NO조적53.7%화25.9%(P<0.05).CA-Ⅸ화암조직화계막조직적VEGF적표체무명현상관(r=0.092화r=-0.292).결론 CA-Ⅸ화VEGF표체여직장암발생、발전화침윤전이유관,단량자작용궤제불동.
Objective To explore the expressions and role of carbonic anhydrases-Ⅸ (CA-Ⅸ) and vascular endothelial growth factor (VEGF) in rectal cancer. Methods The expression levels of CA-Ⅸ and VEGF in 104 pathologically diagnosed rectal cancer tissues, 30 normal tissues, and 30 cases of non-neoplastic rectal mucosa were detected by Western blotting. Results The overall positive expression rate of CA-Ⅸ and VEGF in rectal cancer was 72.1% and 65.4%, repeetively, which was higher than that in non-neoplastic tissue(P<0.05). The expression of CA-Ⅸ was related to Dukes' stage and status of lymph node metastasis. CA-Ⅸ expression positive rate was decreased with the progression of the Dukes' stage from A to C. CA-Ⅸ expression in patients with lymph node negative involvement was higher than that in lymph node involvement, 85.2% vs 58.8%, 56.3%, respectly, with the difference being statistically significant,P<0.05. The expression of VEGF was related to status of lymph node metastasis. VEGF expression in carcinoma tissues of patients with lymph node involvement (81.3%) was significandy higher than that in lymph node negative involvement (53.7%) ,X~2=6.88, P<0.05, and VEGF expression in mesorectal tissues with lymph node involvement (75.0%) was significantly higher than that in lymph node negative involvement (25.9%),X~2=14.52, P<0.05. There was no positive association between CA-Ⅸ and VEGF. Conclusion The hypoxia and angiogenesis are helpful to the judgment of prognosis.