CAJ | 학술논문

目的观察特异性N型胆碱能激动剂烟碱对内脏动脉阻断性(SAO)休克大鼠的保护效应.方法采用阻断肠系膜上动脉和腹腔干动脉45 min,然后开放夹闭动脉恢复再灌注的方法复制SAO休克模型.将40只健康成年雄性SD大鼠随机分为5组:假手术组、休克组、烟碱组、六烃季胺组和α银环蛇毒素(α-BGT)组.恢复再灌注60 min,经左颈总动脉采血用于血浆丙二醛(MDA)含量测定,并无菌留取距回盲瓣10 cm处回肠组织,用于其髓过氧化物酶(MPO)、一氧化氮(NO)、诱导型一氧化氮合酶(iNOS)活性测定和病理学检查.结果与休克组大鼠比较,烟碱组大鼠血浆MDA含量、回肠组织MPO和NO的含量明显减少(P＜0.05),iNOS免疫组织化学吸光度值明显降低(P＜0.01),回肠组织的病理学损伤也明显减轻.经六烃季胺或α-BGT拮抗后,烟碱处理的上述保护效应则被抵消.六烃季胺和α-BGT组比较,大鼠血浆MDA及回肠组织MPO、NO含量和iNOS的表达差异均无统计学意义(P＞0.05).结论烟碱具有减轻SAO休克所致大鼠回肠组织损伤的效应.其作用机制可能与激活胆碱能抗炎通路有关.
목적 관찰특이성N형담감능격동제연감대내장동맥조단성(SAO)휴극대서적보호효응.방법 채용조단장계막상동맥화복강간동맥45 min,연후개방협폐동맥회복재관주적방법복제SAO휴극모형.장40지건강성년웅성SD대서수궤분위5조:가수술조、휴극조、연감조、륙경계알조화α은배사독소(α-BGT)조.회복재관주60 min,경좌경총동맥채혈용우혈장병이철(MDA)함량측정,병무균류취거회맹판10 cm처회장조직,용우기수과양화물매(MPO)、일양화담(NO)、유도형일양화담합매(iNOS)활성측정화병이학검사.결과 여휴극조대서비교,연감조대서혈장MDA함량、회장조직MPO화NO적함량명현감소(P＜0.05),iNOS면역조직화학흡광도치명현강저(P＜0.01),회장조직적병이학손상야명현감경.경륙경계알혹α-BGT길항후,연감처리적상술보호효응칙피저소.륙경계알화α-BGT조비교,대서혈장MDA급회장조직MPO、NO함량화iNOS적표체차이균무통계학의의(P＞0.05).결론 연감구유감경SAO휴극소치대서회장조직손상적효응.기작용궤제가능여격활담감능항염통로유관.
Objective To investigate the beneficial roles of nicotine,a selective cholinergic agonist,on rats with splanchinc artery occlusion (SAO) shock.Methods Forty male anaesthetized SpragueDawley rats,which were randomly divided into 5 groups,were subjected to clamping of the splanchnic arteries for 45 min,followed by release of the clamp to recover reperfusion.This surgical procedure resulted in an irreversible state of shock (SAO shock).Sham operated animals were used as controls.All the animals were implanted with catheter into left common artery to connect pressure transducer for continuously monitoring mean aortic pressure (MAP).At 60 min after reperfusion,all animals were sacrificed for biochemical studies and histological examination.Serum level of malondialdehyde (MDA) and content of myeloperoxidase (MPO) and nitric oxide ( NO),expression of inducible nitric oxide synthase (iNOS) in ileum,and pathologic changes of ileum were examined.Results Compared with SAO shock group,the serum level of MDA,the tissue levels of MPO,NO and iNOS in the ileum were significantly decreased ( P ＜0.05 or P＜0.01 ) and the ileum injury was attenuated in the nicotine group.Administration of specific nAeh receptor antagonist worsened SAO shock-induced tissue injury,and there was no difference in concentration of serum MDA,and in contents of MPO,NO,and iNOS of ileum between two antagonist groups (P ＞ 0.05 ).Conclusion The results suggest that nicotine may play beneficial roles on SAO shock rats through activation of cholinergic anti-inflammatory pathway.